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Protein

Elongation factor TuB, chloroplastic

Gene

TUFB

Organism
Nicotiana sylvestris (Wood tobacco) (South American tobacco)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi95 – 102GTPBy similarity8
Nucleotide bindingi157 – 161GTPBy similarity5
Nucleotide bindingi212 – 215GTPBy similarity4

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuB, chloroplastic
Short name:
EF-TuB
Gene namesi
Name:TUFB
OrganismiNicotiana sylvestris (Wood tobacco) (South American tobacco)
Taxonomic identifieri4096 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 76ChloroplastSequence analysisAdd BLAST76
ChainiPRO_000000745677 – 485Elongation factor TuB, chloroplasticAdd BLAST409

Proteomic databases

PRIDEiQ43364.

Structurei

3D structure databases

ProteinModelPortaliQ43364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 290tr-type GAdd BLAST205

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni95 – 102G1By similarity8
Regioni136 – 140G2By similarity5
Regioni157 – 160G3By similarity4
Regioni212 – 215G4By similarity4
Regioni250 – 252G5By similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

CDDicd03697. EFTU_II. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR033720. EFTU_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43364-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASISAASAT ATASTKLAYP YSPSSSSSSS NTAAVFPSNS SKLILSSSFT
60 70 80 90 100
PTPSTLFLHS PTTTPSTTHP RRFTVRAARG KFERKKPHVN IGTIGHVDHG
110 120 130 140 150
KTTLTAALTM ALASMGNSAP KKYDEIDAAP EERARGITIN TATVEYETEN
160 170 180 190 200
RHYAHVDCPG HADYVKNMIT GAAQMDGAIL VVSGADGPMP QTKEHILLAK
210 220 230 240 250
QVGVPNMVVF LNKQDQVDDE ELLELVELEV RELLSSYEFP GDEIPIISGS
260 270 280 290 300
ALLALEALMA NPSIKRGENQ WVDKIYQLMD NVDEYIPIPQ RQTELPFLMA
310 320 330 340 350
IEDVFSITGR GTVATGRVER GTVKVGEIVD IVGLKDTRNT TVTGVEMFQK
360 370 380 390 400
ILDEAMAGDN VGLLLRGIQK IDIQRGMVLA KPGTITPHTK FEALVYVLKK
410 420 430 440 450
EEGGRHSPFF AGYRPQFYMR TTDVTGKVTV IMSDKGEESK MVMPGDRVNM
460 470 480
VVELIMPVAC EQGMRFAIRE GGKTVGAGVI QKILE
Length:485
Mass (Da):52,688
Last modified:November 1, 1996 - v1
Checksum:iFE88EF5D48B92D29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11470 Genomic DNA. Translation: BAA02028.1.
D11376 mRNA. Translation: BAA01975.1.
PIRiS36184.
RefSeqiXP_009772722.1. XM_009774420.1.

Genome annotation databases

GeneIDi104223071.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11470 Genomic DNA. Translation: BAA02028.1.
D11376 mRNA. Translation: BAA01975.1.
PIRiS36184.
RefSeqiXP_009772722.1. XM_009774420.1.

3D structure databases

ProteinModelPortaliQ43364.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ43364.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi104223071.

Family and domain databases

CDDicd03697. EFTU_II. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR033720. EFTU_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEFTUB_NICSY
AccessioniPrimary (citable) accession number: Q43364
Secondary accession number(s): Q7DNA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.