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Protein

Elongation factor TuB, chloroplastic

Gene

TUFB

Organism
Nicotiana sylvestris (Wood tobacco) (South American tobacco)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi95 – 1028GTPBy similarity
Nucleotide bindingi157 – 1615GTPBy similarity
Nucleotide bindingi212 – 2154GTPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuB, chloroplastic
Short name:
EF-TuB
Gene namesi
Name:TUFB
OrganismiNicotiana sylvestris (Wood tobacco) (South American tobacco)
Taxonomic identifieri4096 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7676ChloroplastSequence analysisAdd
BLAST
Chaini77 – 485409Elongation factor TuB, chloroplasticPRO_0000007456Add
BLAST

Proteomic databases

PRIDEiQ43364.

Structurei

3D structure databases

ProteinModelPortaliQ43364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini86 – 290205tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni95 – 1028G1By similarity
Regioni136 – 1405G2By similarity
Regioni157 – 1604G3By similarity
Regioni212 – 2154G4By similarity
Regioni250 – 2523G5By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

CDDicd03697. EFTU_II. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR033720. EFTU_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43364-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASISAASAT ATASTKLAYP YSPSSSSSSS NTAAVFPSNS SKLILSSSFT
60 70 80 90 100
PTPSTLFLHS PTTTPSTTHP RRFTVRAARG KFERKKPHVN IGTIGHVDHG
110 120 130 140 150
KTTLTAALTM ALASMGNSAP KKYDEIDAAP EERARGITIN TATVEYETEN
160 170 180 190 200
RHYAHVDCPG HADYVKNMIT GAAQMDGAIL VVSGADGPMP QTKEHILLAK
210 220 230 240 250
QVGVPNMVVF LNKQDQVDDE ELLELVELEV RELLSSYEFP GDEIPIISGS
260 270 280 290 300
ALLALEALMA NPSIKRGENQ WVDKIYQLMD NVDEYIPIPQ RQTELPFLMA
310 320 330 340 350
IEDVFSITGR GTVATGRVER GTVKVGEIVD IVGLKDTRNT TVTGVEMFQK
360 370 380 390 400
ILDEAMAGDN VGLLLRGIQK IDIQRGMVLA KPGTITPHTK FEALVYVLKK
410 420 430 440 450
EEGGRHSPFF AGYRPQFYMR TTDVTGKVTV IMSDKGEESK MVMPGDRVNM
460 470 480
VVELIMPVAC EQGMRFAIRE GGKTVGAGVI QKILE
Length:485
Mass (Da):52,688
Last modified:November 1, 1996 - v1
Checksum:iFE88EF5D48B92D29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11470 Genomic DNA. Translation: BAA02028.1.
D11376 mRNA. Translation: BAA01975.1.
PIRiS36184.
RefSeqiXP_009772722.1. XM_009774420.1.

Genome annotation databases

GeneIDi104223071.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11470 Genomic DNA. Translation: BAA02028.1.
D11376 mRNA. Translation: BAA01975.1.
PIRiS36184.
RefSeqiXP_009772722.1. XM_009774420.1.

3D structure databases

ProteinModelPortaliQ43364.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ43364.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi104223071.

Family and domain databases

CDDicd03697. EFTU_II. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR033720. EFTU_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEFTUB_NICSY
AccessioniPrimary (citable) accession number: Q43364
Secondary accession number(s): Q7DNA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.