Reviewed, UniProtKB/Swiss-Prot Q43314 (DHE1_ARATH)

Names and origin

Protein names

Recommended name:
    Glutamate dehydrogenase 1
      Short name=GDH 1
    EC=1.4.1.3

Gene names

Name:	GDH1
Ordered Locus Names:	At5g18170
ORF Names:	MRG7.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to absence of light Inferred from expression pattern. Source: TAIR response to cadmium ion Inferred from expression pattern. Source: TAIR response to salt stress Inferred from expression pattern. Source: TAIR
Cellular component	mitochondrion Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from direct assay. Source: TAIR glutamate dehydrogenase [NAD(P)+] activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Glutamate dehydrogenase 1		PRO_0000182745
Sites
	Active site	102	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43314-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4F14E31CEC52935E	FASTA	411	44,524
	10 20 30 40 50 60 MNALAATNRN FKLAARLLGL DSKLEKSLLI PFREIKVECT IPKDDGTLAS FVGFRVQHDN 70 80 90 100 110 120 ARGPMKGGIR YHPEVDPDEV NALAQLMTWK TAVAKIPYGG AKGGIGCDPS KLSISELERL 130 140 150 160 170 180 TRVFTQKIHD LIGIHTDVPA PDMGTGPQTM AWILDEYSKF HGYSPAVVTG KPIDLGGSLG 190 200 210 220 230 240 RDAATGRGVM FGTEALLNEH GKTISGQRFV IQGFGNVGSW AAKLISEKGG KIVAVSDITG 250 260 270 280 290 300 AIKNKDGIDI PALLKHTKEH RGVKGFDGAD PIDPNSILVE DCDILVPAAL GGVINRENAN 310 320 330 340 350 360 EIKAKFIIEA ANHPTDPDAD EILSKKGVVI LPDIYANSGG VTVSYFEWVQ NIQGFMWEEE 370 380 390 400 410 KVNDELKTYM TRSFKDLKEM CKTHSCDLRM GAFTLGVNRV AQATILRGWG A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis glutamate dehydrogenase 1." Thakkar S., Weisemann J.M., Turano F.J. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Arabidopsis mutant analysis and gene regulation define a nonredundant role for glutamate dehydrogenase in nitrogen assimilation." Melo-Oliveira R., Oliveira I., Coruzzi G. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U37771 mRNA. Translation: AAA82615.1. U53527 mRNA. Translation: AAB08057.1. AB012246 Genomic DNA. Translation: BAB09475.1.
IPI	IPI00531762.
PIR	S71217.
RefSeq	NP_197318.1.
UniGene	At.23609
3D structure databases
HSSP	HSSP built from PDB template 1B26 based on UniProtKB P96110.
ModBase	Search...
Proteomic databases
PRIDE	Q43314.
ProMEX	Q43314.
Genome annotation databases
GeneID	831935.
GenomeReviews	Gene locus AT5G18170 in contig BA000015_GR.
KEGG	ath:AT5G18170.
NMPDR	fig|3702.1.peg.23989.
Organism-specific databases
GeneFarm	2257. 178.
TAIR	At5g18170.
Phylogenomic databases
OMA	Q43314. GIRIDPR.
Enzyme and pathway databases
BRENDA	1.4.1.3. 302.
Gene expression databases
ArrayExpress	Q43314.
GermOnline	AT5G18170. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE1_ARATH

Accession

Primary (citable) accession number: Q43314

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)