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Protein

1-aminocyclopropane-1-carboxylate synthase 4

Gene

ACS4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene.

Miscellaneous

The stability of ACS proteins, and the regulation of such stability, play a central role in ethylene biosynthesis.

Catalytic activityi

S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + methylthioadenosine.1 Publication

Cofactori

Kineticsi

  1. KM=15 µM for AdoMet
  1. Vmax=31.20 µM/h/mg enzyme

pH dependencei

Optimum pH is 7.8.

Pathwayi: ethylene biosynthesis via S-adenosyl-L-methionine

This protein is involved in step 1 of the subpathway that synthesizes ethylene from S-adenosyl-L-methionine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 1-aminocyclopropane-1-carboxylate synthase 4 (ACS4), 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8), 1-aminocyclopropane-1-carboxylate synthase 2 (ACS2), 1-aminocyclopropane-1-carboxylate synthase 7 (ACS7), 1-aminocyclopropane-1-carboxylate synthase 11 (ACS11), 1-aminocyclopropane-1-carboxylate synthase 9 (ACS9), 1-aminocyclopropane-1-carboxylate synthase 6 (ACS6), 1-aminocyclopropane-1-carboxylate synthase 5 (ACS5)
  2. 1-aminocyclopropane-1-carboxylate oxidase 2 (ACO2), 1-aminocyclopropane-1-carboxylate oxidase 3 (At1g12010), 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4), 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1), 1-aminocyclopropane-1-carboxylate oxidase 5 (At1g77330)
This subpathway is part of the pathway ethylene biosynthesis via S-adenosyl-L-methionine, which is itself part of Alkene biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ethylene from S-adenosyl-L-methionine, the pathway ethylene biosynthesis via S-adenosyl-L-methionine and in Alkene biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei47SubstrateBy similarity1
Binding sitei85SubstrateBy similarity1

GO - Molecular functioni

  • 1-aminocyclopropane-1-carboxylate synthase activity Source: TAIR
  • identical protein binding Source: IntAct
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • cellular response to iron ion Source: TAIR
  • ethylene biosynthetic process Source: UniProtKB-UniPathway
  • fruit ripening Source: UniProtKB-KW

Keywordsi

Molecular functionLyase
Biological processEthylene biosynthesis, Fruit ripening
LigandPyridoxal phosphate, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT2G22810-MONOMER
MetaCyc:AT2G22810-MONOMER
SABIO-RKQ43309
UniPathwayiUPA00384; UER00562

Names & Taxonomyi

Protein namesi
Recommended name:
1-aminocyclopropane-1-carboxylate synthase 4 (EC:4.4.1.14)
Short name:
ACC synthase 4
Alternative name(s):
S-adenosyl-L-methionine methylthioadenosine-lyase 4
Gene namesi
Name:ACS4
Synonyms:ACC4
Ordered Locus Names:At2g22810
ORF Names:T20K9.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G22810
TAIRilocus:2059170 AT2G22810

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001238981 – 4741-aminocyclopropane-1-carboxylate synthase 4Add BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei273N6-(pyridoxal phosphate)lysineBy similarity1

Post-translational modificationi

Ubiquitinated by XBAT32. Ubiquitination probably leads to its subsequent degradation, thus controlling ethylene production.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ43309

Expressioni

Tissue specificityi

Expressed in roots, leaves and flowers.1 Publication

Inductioni

By indole-3-acetic acid (IAA) and cycloheximide (CHX). By auxin.2 Publications

Gene expression databases

ExpressionAtlasiQ43309 baseline and differential
GenevisibleiQ43309 AT

Interactioni

Subunit structurei

Homodimer and heterodimer. In vivo, the relevance of heterodimerization with other ACS enzymes is however unsure (By similarity). Interacts with XBAT32. Interacts (via its C-terminal region) with ETO1 and EOL1.By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi2165, 2 interactors
IntActiQ43309, 5 interactors
STRINGi3702.AT2G22810.1

Structurei

3D structure databases

ProteinModelPortaliQ43309
SMRiQ43309
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0256 Eukaryota
COG0436 LUCA
HOGENOMiHOG000011234
InParanoidiQ43309
KOiK01762
OMAiQANEAMH
OrthoDBiEOG093608EZ
PhylomeDBiQ43309

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR004838 NHTrfase_class1_PyrdxlP-BS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00105 AA_TRANSFER_CLASS_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q43309-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQLSRKATC NSHGQVSSYF LGWEEYEKNP YDVTKNPQGI IQMGLAENQL
60 70 80 90 100
CFDLLESWLA QNTDAACFKR DGQSVFRELA LFQDYHGLSS FKNAFADFMS
110 120 130 140 150
ENRGNRVSFD SNNLVLTAGA TSANETLMFC LADPGDAFLL PTPYYPGFDR
160 170 180 190 200
DLKWRTGVEI VPIQSSSTNG FRITKLALEE AYEQAKKLDL NVKGILITNP
210 220 230 240 250
SNPLGTTTTQ TELNILFDFI TKNKNIHLVS DEIYSGTVFN SSEFISVMEI
260 270 280 290 300
LKNNQLENTD VLNRVHIVCS LSKDLGLPGF RVGAIYSNDK DVISAATKMS
310 320 330 340 350
SFGLVSSQTQ YLLSSLLSDK KFTKNYLREN QKRLKNRQRK LVLGLEAIGI
360 370 380 390 400
KCLKSNAGLF CWVDMRPLLR SKTFEAEMDL WKKIVYEVKL NISPGSSCHC
410 420 430 440 450
EEPGWFRVCF ANMIDETLKL ALKRLKMLVD DENSSRRCQK SKSERLNGSR
460 470
KKTMSNVSNW VFRLSFHDRE AEER
Length:474
Mass (Da):53,795
Last modified:November 1, 1996 - v1
Checksum:i98FD27622EAF3C21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23481 mRNA Translation: AAC49037.1
U23482 Genomic DNA Translation: AAA85039.1
AC004786 Genomic DNA Translation: AAC32428.1
AC005617 Genomic DNA Translation: AAM15065.1
CP002685 Genomic DNA Translation: AEC07360.1
AF332404 mRNA Translation: AAG48767.1
PIRiB84617
G46376
RefSeqiNP_179866.1, NM_127846.2
UniGeneiAt.1549

Genome annotation databases

EnsemblPlantsiAT2G22810.1; AT2G22810.1; AT2G22810
GeneIDi816812
GrameneiAT2G22810.1; AT2G22810.1; AT2G22810
KEGGiath:AT2G22810

Similar proteinsi

Entry informationi

Entry namei1A14_ARATH
AccessioniPrimary (citable) accession number: Q43309
Secondary accession number(s): Q9S9C4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health