Reviewed, UniProtKB/Swiss-Prot Q43299 (CAPP_AMAHP)

Names and origin

Protein names	Recommended name:     Phosphoenolpyruvate carboxylase       Short name=PEPCase       Short name=PEPC     EC=4.1.1.31
Organism	Amaranthus hypochondriacus (Prince-of-Wales feather)
Taxonomic identifier	28502 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Amaranthus

Protein attributes

Sequence length	964 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + CO2.
Enzyme regulation	By light-reversible phosphorylation By similarity.
Pathway	Photosynthesis; C4 acid pathway.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the PEPCase type 1 family.

Ontologies

Keywords
Biological process	Carbon dioxide fixation Photosynthesis Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
PTM	Phosphoprotein
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	carbon utilization by fixation of carbon dioxide Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 964	964	Phosphoenolpyruvate carboxylase		PRO_0000166656
Sites
	Active site	172	1	By similarity
	Active site	600	1	By similarity
Amino acid modifications
	Modified residue	11	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43299-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 3D49C2DE8BDE11B3	FASTA	964	109,480
	10 20 30 40 50 60 MASGKVEKMA SIDAQLRLLA PKKVSEDDKL VEYDALLLDR FLDILESLHG SGIRETVQEL 70 80 90 100 110 120 YEHAAEYERT HDTKKLEELG NLITSLDAGD SIVIAKSFSQ MLNLANLAEE VQLAYRRRIK 130 140 150 160 170 180 KTKKGDFADE SSAITESDFE ETLRRLVDLK KSPEEIFATL KNQTVDLVLT AHPTQSVRRS 190 200 210 220 230 240 LLQKHGRIRD CLSQLYAKDI SPDDKQELDE ALQRAIQAAF RTDEIRRVQP TPQDEMRMGM 250 260 270 280 290 300 SYFHETIWKG VPKFLRRVDT ALKNIGINER VPYNVPLIQF SSWMGGDRDG NPRVTPEVTR 310 320 330 340 350 360 DVVLLARMMA ANMYFTQITD LMFELSMWRC NDEVRARAQE LHSQSKSDAK HYIEFWKQIP 370 380 390 400 410 420 LSEPYRVILG DVRDKLYNTR EHAHKLLANG SSDVPEESTF THIDQFLEPL ELCYKSLCAS 430 440 450 460 470 480 GDQPIADGSL LDFMRQVSTF GLSLVKLDIR QESDRHTEVM DAITTHLGIG SYRSWSEEKR 490 500 510 520 530 540 QEWLLSELRG KRPLFGSDLP MSYEVADAIG TFRVLAELPN DSFGAYIISM ATAPSDVLAV 550 560 570 580 590 600 ELLQRECGIK KPLRVVPLFE KLADLQSAAA SMTRLFSIDW YKNRINGTQE VMIGYSDSGK 610 620 630 640 650 660 DAGRLSAAWQ LYKVQEQLIQ VAKEYGVKLT MFHGRGGTVG RGGGPTHLAL LSQPPDTIHG 670 680 690 700 710 720 SLRVTIQGEV IEQSFGEEHL CFRTLERYTA ATLEHGIDPP TSPKPEWRAL MDEMAVITTK 730 740 750 760 770 780 EYRSVVLQEP RFVEYFRSAT PELEYGRMNI GSRPAKRKPG GGIETLRAIP WIFSWTQTRF 790 800 810 820 830 840 HLPVWLGCGA AFKHVIEKDI KNLAMLKDMY NQWSFFRVTI DLLEMVFAKG DPGIAALYDK 850 860 870 880 890 900 LLVKDELKPF GENLRKSYLE AQKFLLEIAG HKDPLDADPY LKQILRLRDP YTTTLNVFQV 910 920 930 940 950 960 YTLKRIRDPS FHVTVRPHLS KEMDANSLAA DLVKLNPTSE YPPGLEDTLI LTMKGIAAGM QNTG

References

[1]	"The C4 phosphoenolpyruvate carboxylase (PEPCase) from grain Amaranth." Rydzik E., Berry J. Plant Gene Register PGR95-135 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z68125 mRNA. Translation: CAA92209.1. L49175 mRNA. Translation: AAB18633.1.
3D structure databases
HSSP	HSSP built from PDB template 1JQO based on UniProtKB P04711.
SMR	Q43299. Positions 31-964.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.1.1.31. 2722.
Family and domain databases
InterPro	IPR001449. PEP_COase. IPR018129. PEP_COase_AS. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
Pfam	PF00311. PEPcase. 1 hit. [Graphical view]
PRINTS	PR00150. PEPCARBXLASE.
PROSITE	PS00781. PEPCASE_1. 1 hit. PS00393. PEPCASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAPP_AMAHP

Accession

Primary (citable) accession number: Q43299

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)