Reviewed, UniProtKB/Swiss-Prot Q43295 (KAP1_ARATH)

Names and origin

Protein names

Recommended name:
    Adenylyl-sulfate kinase 1, chloroplastic
    EC=2.7.1.25
Alternative name(s):
    Adenosine-5'-phosphosulfate kinase
      Short name=APS kinase
    ATP adenosine-5'-phosphosulfate 3'-phosphotransferase

Gene names

Name:	AKN1
Ordered Locus Names:	At2g14750
ORF Names:	F26C24.11, T6B13.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the synthesis of activated sulfate.
Catalytic activity	ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the APS kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW phosphorylation Inferred from direct assay. Source: TAIR regulation of glucosinolate biosynthetic process Inferred from genetic interaction. Source: TAIR sulfate assimilation Inferred from genetic interaction. Source: TAIR
Cellular component	chloroplast Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylylsulfate kinase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 37	37	Chloroplast Potential
	Chain	38 – 276	239	Adenylyl-sulfate kinase 1, chloroplastic		PRO_0000006635
Regions
	Nucleotide binding	108 – 115	8	ATP Potential
Sites
	Active site	182	1	Phosphoserine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43295-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CB698643AA09D811	FASTA	276	29,787
	10 20 30 40 50 60 MIAAGAKSLL GLSMASPKGI FDSNSMSNSR SVVVVRACVS MDGSQTLSHN KNGSIPEVKS 70 80 90 100 110 120 INGHTGQKQG PLSTVGNSTN IKWHECSVEK VDRQRLLDQK GCVIWVTGLS GSGKSTLACA 130 140 150 160 170 180 LNQMLYQKGK LCYILDGDNV RHGLNRDLSF KAEDRAENIR RVGEVAKLFA DAGIICIASL 190 200 210 220 230 240 ISPYRTDRDA CRSLLPEGDF VEVFMDVPLS VCEARDPKGL YKLARAGKIK GFTGIDDPYE 250 260 270 PPLNCEISLG REGGTSPIEM AEKVVGYLDN KGYLQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cDNA for adenylyl sulphate (APS)-kinase from Arabidopsis thaliana." Arz H.E., Gisselmann G., Schiffmann S., Schwenn J.-D. Biochim. Biophys. Acta 1218:447-452(1994) [PubMed: 8049272] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[2]	"A cDNA clone for 5'-adenylylphosphosulfate kinase from Arabidopsis thaliana." Jain A., Leustek T. Plant Physiol. 105:771-772(1994) [PubMed: 8066145] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"APS kinase from Arabidopsis thaliana: genomic organization, expression, and kinetic analysis of the recombinant enzyme." Lee S., Leustek T. Biochem. Biophys. Res. Commun. 247:171-175(1998) [PubMed: 9636674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta.
[4]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75782 mRNA. Translation: CAA53426.1. U05238 mRNA. Translation: AAC50035.1. U59759 Genomic DNA. Translation: AAC50034.1. AC004705 Genomic DNA. Translation: AAC24182.1. AY054287 mRNA. Translation: AAL06946.1. AY132010 mRNA. Translation: AAM91043.1.
IPI	IPI00516504.
PIR	S47640.
RefSeq	NP_179082.1.
UniGene	At.10821
3D structure databases
HSSP	HSSP built from PDB template 1M7G based on UniProtKB Q12657.
ModBase	Search...
Proteomic databases
PRIDE	Q43295.
Genome annotation databases
GeneID	815963.
GenomeReviews	Gene locus AT2G14750 in contig CT485783_GR.
KEGG	ath:AT2G14750.
NMPDR	fig|3702.1.peg.8486.
Organism-specific databases
TAIR	At2g14750.
Phylogenomic databases
OMA	Q43295. DAGIICI.
Enzyme and pathway databases
BRENDA	2.7.1.25. 302.
Gene expression databases
ArrayExpress	Q43295.
GermOnline	AT2G14750. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002891. APS_kinase_C. [Graphical view]
Pfam	PF01583. APS_kinase. 1 hit. [Graphical view]
ProDom	PD002350. APS_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00455. apsK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KAP1_ARATH

Accession

Primary (citable) accession number: Q43295

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)