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Protein

Adenylyl-sulfate kinase 1, chloroplastic

Gene

APK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of activated sulfate. Essential for plant reproduction and viability. Required for the production of glucosinolates.5 Publications

Catalytic activityi

ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.

Kineticsi

  1. KM=0.14 µM for adenylyl sulfate2 Publications
  2. KM=5.1 µM for adenylyl sulfate2 Publications
  3. KM=147 µM for ATP2 Publications
  1. Vmax=7.35 mmol/min/mg enzyme2 Publications
  2. Vmax=20.1 mmol/min/mg enzyme2 Publications

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. ATP sulfurylase 4, chloroplastic (APS4), ATP-sulfurylase 3, chloroplastic (APS3), ATP sulfurylase 2 (APS2), ATP sulfurylase 1, chloroplastic (APS1)
  2. Adenylyl-sulfate kinase 4, chloroplastic (APK4), Adenylyl-sulfate kinase 3 (APK3), Adenylyl-sulfate kinase (APK4), Adenylyl-sulfate kinase (AXX17_At3g03310), Adenylyl-sulfate kinase (AXX17_At2g09910), Adenylyl-sulfate kinase 1, chloroplastic (APK1), Adenylyl-sulfate kinase (APK3), Adenylyl-sulfate kinase (AXX17_At4g45360), Adenylyl-sulfate kinase 2, chloroplastic (APK2), Adenylyl-sulfate kinase (AXX17_At5g67630)
  3. no protein annotated in this organism
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei138Substrate2 Publications1
Binding sitei141Substrate2 Publications1
Sitei150Participates in a stacking interaction with the adenine ring of adenylyl-sulfate1
Binding sitei155Substrate2 Publications1
Binding sitei158Substrate2 Publications1
Active sitei182Phosphoserine intermediateBy similarity1
Binding sitei231Substrate; via carbonyl oxygen2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi108 – 116ATP1 Publication9

GO - Molecular functioni

  • adenylylsulfate kinase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G14750-MONOMER
MetaCyc:AT2G14750-MONOMER
BRENDAi2.7.1.25 399
UniPathwayiUPA00140; UER00205

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylyl-sulfate kinase 1, chloroplastic (EC:2.7.1.25)
Alternative name(s):
ATP adenosine-5'-phosphosulfate 3'-phosphotransferase 1
Adenosine-5'-phosphosulfate kinase 1
Short name:
APS kinase 1
Gene namesi
Name:APK1
Synonyms:AKN1
Ordered Locus Names:At2g14750
ORF Names:F26C24.11, T6B13.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G14750
TAIRilocus:2046901 AT2G14750

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions. Apk1 and apk2 double mutant exhibits a semi-dwarf phenotype.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi86C → A: Increased catalytic efficiency in oxidative conditions; when associated with Ala-119. 1 Publication1
Mutagenesisi119C → A: Increased catalytic efficiency in oxidative conditions; when associated with Ala-86. 1 Publication1
Mutagenesisi136D → A or N: Decreases affinity for substrate and catalytic efficiency. 1 Publication1
Mutagenesisi182S → C or F: No effect on catalytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 38ChloroplastSequence analysisAdd BLAST38
ChainiPRO_000000663539 – 276Adenylyl-sulfate kinase 1, chloroplasticAdd BLAST238

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi86Interchain (with C-119)1 Publication
Disulfide bondi119Interchain (with C-86)1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ43295

PTM databases

iPTMnetiQ43295

Expressioni

Tissue specificityi

Expressed in root vasculature, root tips, leaf epidermal and guard cells, pollen grains and funiculus of developing seeds.1 Publication

Gene expression databases

ExpressionAtlasiQ43295 baseline and differential
GenevisibleiQ43295 AT

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with APK2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-4438040,EBI-4438040

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-60003N
IntActiQ43295, 2 interactors
STRINGi3702.AT2G14750.1

Structurei

Secondary structure

1276
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi90 – 97Combined sources8
Beta strandi102 – 107Combined sources6
Helixi114 – 127Combined sources14
Beta strandi132 – 136Combined sources5
Helixi137 – 140Combined sources4
Turni141 – 147Combined sources7
Helixi152 – 171Combined sources20
Beta strandi175 – 179Combined sources5
Helixi185 – 193Combined sources9
Beta strandi199 – 205Combined sources7
Helixi209 – 215Combined sources7
Helixi220 – 225Combined sources6
Turni233 – 235Combined sources3
Beta strandi246 – 249Combined sources4
Helixi257 – 271Combined sources15
Turni272 – 274Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UIEX-ray1.79A/B/C77-276[»]
4FXPX-ray1.95A/B/C77-276[»]
ProteinModelPortaliQ43295
SMRiQ43295
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni181 – 182Substrate binding2

Sequence similaritiesi

Belongs to the APS kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0635 Eukaryota
COG0529 LUCA
HOGENOMiHOG000228204
KOiK00860
OMAiCVSMDGS
OrthoDBiEOG09360JIA
PhylomeDBiQ43295

Family and domain databases

CDDicd02027 APSK, 1 hit
HAMAPiMF_00065 Adenylyl_sulf_kinase, 1 hit
InterProiView protein in InterPro
IPR002891 APS_kinase
IPR027417 P-loop_NTPase
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00455 apsK, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAAGAKSLL GLSMASPKGI FDSNSMSNSR SVVVVRACVS MDGSQTLSHN
60 70 80 90 100
KNGSIPEVKS INGHTGQKQG PLSTVGNSTN IKWHECSVEK VDRQRLLDQK
110 120 130 140 150
GCVIWVTGLS GSGKSTLACA LNQMLYQKGK LCYILDGDNV RHGLNRDLSF
160 170 180 190 200
KAEDRAENIR RVGEVAKLFA DAGIICIASL ISPYRTDRDA CRSLLPEGDF
210 220 230 240 250
VEVFMDVPLS VCEARDPKGL YKLARAGKIK GFTGIDDPYE PPLNCEISLG
260 270
REGGTSPIEM AEKVVGYLDN KGYLQA
Length:276
Mass (Da):29,787
Last modified:November 1, 1996 - v1
Checksum:iCB698643AA09D811
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41M → I in AAM62496 (Ref. 7) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75782 mRNA Translation: CAA53426.1
U05238 mRNA Translation: AAC50035.1
U59759 Genomic DNA Translation: AAC50034.1
AC004705 Genomic DNA Translation: AAC24182.1
CP002685 Genomic DNA Translation: AEC06330.1
AY054287 mRNA Translation: AAL06946.1
AY132010 mRNA Translation: AAM91043.1
AY085264 mRNA Translation: AAM62496.1
PIRiS47640
RefSeqiNP_179082.1, NM_127039.4
UniGeneiAt.10821

Genome annotation databases

EnsemblPlantsiAT2G14750.1; AT2G14750.1; AT2G14750
GeneIDi815963
GrameneiAT2G14750.1; AT2G14750.1; AT2G14750
KEGGiath:AT2G14750

Similar proteinsi

Entry informationi

Entry nameiAPK1_ARATH
AccessioniPrimary (citable) accession number: Q43295
Secondary accession number(s): Q8LES2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health