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Reviewed, UniProtKB/Swiss-Prot Q43272 (GAPN_MAIZE)

Last modified June 16, 2009. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    NADP-dependent glyceraldehyde-3-phosphate dehydrogenase
    EC=1.2.1.9
Alternative name(s):
    Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase
    Glyceraldehyde-3-phosphate dehydrogenase [NADP+]
    Triosephosphate dehydrogenase
Gene names
Name: GPN1
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

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Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Important as a means of generating NADPH for biosynthetic reactions.

Catalytic activity

D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

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Ontologies

Keywords
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionglyceraldehyde-3-phosphate dehydrogenase (NADP+) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 498498NADP-dependent glyceraldehyde-3-phosphate dehydrogenase
PRO_0000056576

Regions

Nucleotide binding247 – 2515NAD By similarity
Region171 – 1722Substrate binding By similarity
Region299 – 3013Substrate binding By similarity

Sites

Active site2661Proton acceptor By similarity
Active site3001Nucleophile By similarity
Binding site1181Substrate By similarity
Binding site1941NADP By similarity
Binding site1971NADP By similarity
Binding site2321NADP By similarity
Binding site3931NADP By similarity
Binding site4531Substrate By similarity
Site1711Transition state stabilizer By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q43272-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7AF1C0DACAB4EE39

FASTA49853,146
        10         20         30         40         50         60 
MALAGTGVFA EILDGEVYRY YADGEWRTSA SGKSVAIVNP TTRKTQYRVQ ACTQEEVNKA 

        70         80         90        100        110        120 
MDAAKVAQKA WARTPLWKRA DVLHKAAAIL KEHKAPIAEC LVKEIAKPAK DAVSEVVRSG 

       130        140        150        160        170        180 
DLVSYTAEEG VRILGEGKLV VSDSFPGNER NKYCLSSKIP LGVVLAIPPF NYPANLAGSK 

       190        200        210        220        230        240 
IGPALIAGNA LVLKPPTQGA VAALHMVHCF HLAGFPKGLI SCVTGKGSEI GDFLTMHPGV 

       250        260        270        280        290        300 
NCISFTGGDT GIAISKKAGM VPLQMELGGK DACIVLEDAD LDLVSANIVK GGFSYSGQRC 

       310        320        330        340        350        360 
TAVKVVLIME SIADAVVQKV NAKLAKLKVG PPEDDSDITP VVTESSANFI EGLVMDAKEK 

       370        380        390        400        410        420 
GATFCQEYRR EGNLIWPLLL DHVRPDMRIA WEEPFGPVLP VIRINSVEEG IHHCNASNFG 

       430        440        450        460        470        480 
LQGCIFTRDI NKAILISDAM ETGTVQINSA PARGPDHFSF QGLKDSGIGS QGITNSINMM 

       490 
TKVKSTVINL PSPSYTMG

« Hide

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References

[1]"Non-phosphorylating GAPDH of higher plants is a member of the aldehyde dehydrogenase superfamily with no sequence homology to phosphorylating GAPDH."
Habenicht A., Hellman U., Cerff R.
J. Mol. Biol. 237:165-171(1994) [PubMed: 7545914] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. KW5330.
Tissue: Shoot.

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Cross-references

Sequence databases

X75326 mRNA. Translation: CAA53075.1.
PIRS43833.
RefSeqNP_001105589.1.
UniGeneZm.175

3D structure databases

HSSPHSSP built from PDB template 1EUH based on UniProtKB Q59931.
ModBaseSearch...

Genome annotation databases

GeneID542583.

Organism-specific databases

GrameneQ43272.
MaizeGDB78926.

Enzyme and pathway databases

BRENDA1.2.1.9. 289.

Family and domain databases

InterProIPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH.
[Graphical view]
Gene3DG3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHERPTHR11699. Aldehyde_dehyd. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameGAPN_MAIZE
AccessionPrimary (citable) accession number: Q43272
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents