Reviewed, UniProtKB/Swiss-Prot Q43270 (PLDA1_MAIZE)

Names and origin

Protein names

Recommended name:
    Phospholipase D alpha 1
      Short name=PLD alpha 1
    EC=3.1.4.4
Alternative name(s):
    Choline phosphatase 1
    Phosphatidylcholine-hydrolyzing phospholipase D 1

Gene names

Name:

PLD1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	812 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Cofactor	Calcium By similarity.
Subunit structure	Monomer By similarity.
Domain	C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.
Sequence similarities	Belongs to the phospholipase D family. C2-PLD subfamily. Contains 1 C2 domain. Contains 2 PLD phosphodiesterase domains.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Repeat
Ligand	Calcium
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylcholine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 812	812	Phospholipase D alpha 1		PRO_0000218820
Regions
	Domain	1 – 114	114	C2
	Domain	330 – 368	39	PLD phosphodiesterase 1
	Domain	658 – 685	28	PLD phosphodiesterase 2
Sites
	Active site	335	1	Potential
	Active site	337	1	Potential
	Active site	342	1	Potential
	Active site	663	1	Potential
	Active site	665	1	Potential
	Active site	670	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q43270-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: D05CB351655BCC61	FASTA	812	92,242
	10 20 30 40 50 60 MAQILLHGTL HATIFEAESL SNPHRATGGA PKFIRKLVEG IEDTVGVGKG ATKIYATVDL 70 80 90 100 110 120 EKARVGRTRM ISNEPVNPRW YESFHIYCAH MAADVIFTVK IDNSIGASLI GRAYLAVQDL 130 140 150 160 170 180 LGGEEIDKWL EISDENREPV GDSKIHVKLQ YFDVGKDRNW ARGVRSTKYP GVPYTFFSQR 190 200 210 220 230 240 QGCKVTLYQD AHVPDNFVPR IQLADGKNYE PHRCWEDIFD AISKAQHLIY ITGWSVYTEI 250 260 270 280 290 300 TLVRDTNRPK PGGDVTLGEL LKRKASEGVR VLMLVWDDRT SVGLLKKDGL MATHDEETAN 310 320 330 340 350 360 YFHGTDVNCV LCPRNPDDSG SFVQDLQIST MFTHHQKIVV VDHEMPNQGS QQRRIVSFIG 370 380 390 400 410 420 GIDLCDGRYD TQYHSLFRTL DTVHHDDFHQ PNFEGGSIKK GGPREPWHDI HSRLEGPIAW 430 440 450 460 470 480 DVLYNFEQRW RKQGGKDLLV RLRDLPDIII PPSPVMFPED RETWNVQLFR SIDGGAAFGF 490 500 510 520 530 540 PETPEEAARA GLVSGKDQII DRSIQDAYVN AIRRAKNFIY IENQYFLGSS YGWKPEGIKP 550 560 570 580 590 600 EEIGALHLIP KELSLKIVSK IEAGERFTVY VVVPMWPEGV PESASVQAIL DWQRRTMEMM 610 620 630 640 650 660 YTDIAQALEA NGIEANPKDY LTFFCLGNRE VKQEGEYEPE EHPEPDTDYI RAQEARRFMI 670 680 690 700 710 720 YVHTKMMIVD DEYIIIGSAN INQRSMDGAR DSEIAMGAYQ PYHLATRQPA RGQIHGFRMS 730 740 750 760 770 780 LWYEHLGMLE DVFQRPESVE CVQKVNEVAE KYWDLYSSDD LEQDLPGHLL SYPIGVTADG 790 800 810 SVTELPGMEN FPDTRARVLG NKSDYLPPIL TT

References

[1]

"Purification and characterization of phospholipase D (PLD) from rice (Oryza sativa L.) and cloning of cDNA for PLD from rice and maize (Zea mays L.)." Ueki J., Morioka S., Komari T., Kumashiro T. Plant Cell Physiol. 36:903-914(1995) [PubMed: 7551587] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Missouri 17.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D73410 mRNA. Translation: BAA11135.1.
PIR	T03659.
RefSeq	NP_001105686.1.
UniGene	Zm.95980
3D structure databases
SMR	Q43270. Positions 5-151, 661-690.
ModBase	Search...
Proteomic databases
PRIDE	Q43270.
Genome annotation databases
GeneID	542702.
KEGG	zma:542702.
Organism-specific databases
Gramene	Q43270.
MaizeGDB	113853.
Enzyme and pathway databases
BRENDA	3.1.4.4. 289.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR015679. Phospholipase_D. IPR011402. PLD_pln. IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00168. C2. 1 hit. PF00614. PLDc. 2 hits. [Graphical view]
PIRSF	PIRSF036470. PLD_plant. 1 hit.
SMART	SM00239. C2. 1 hit. SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50004. C2. False negative. PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLDA1_MAIZE

Accession

Primary (citable) accession number: Q43270

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 19, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)