ID   DHE3_MAIZE              Reviewed;         411 AA.
AC   Q43260; O04871; O04872;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 56.
DE   RecName: Full=Glutamate dehydrogenase;
DE            Short=GDH;
DE            EC=1.4.1.3;
GN   Name=GDH1;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae;
OC   PACCAD clade; Panicoideae; Andropogoneae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Golden cross Bantam T51; TISSUE=Root;
RX   MEDLINE=96012931; PubMed=7551585;
RA   Sakakibara H., Fujii K., Sugiyama T.;
RT   "Isolation and characterization of a cDNA that encodes maize glutamate
RT   dehydrogenase.";
RL   Plant Cell Physiol. 36:789-797(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND MUTANT GDH1.
RC   STRAIN=cv. P-10 Inbred; TISSUE=Shoot;
RA   Ju G.C.;
RT   "Characterization of a GDH1-mutant in maize.";
RL   Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: L-glutamate + H(2)O + NAD(P)(+) = 2-
CC       oxoglutarate + NH(3) + NAD(P)H.
CC   -!- SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
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DR   EMBL; D49475; BAA08445.1; -; mRNA.
DR   EMBL; U93560; AAB51595.1; -; mRNA.
DR   EMBL; U93561; AAB51596.1; -; mRNA.
DR   PIR; T03294; T03294.
DR   PIR; T04342; T04342.
DR   RefSeq; NP_001105301.1; -.
DR   UniGene; Zm.44; -.
DR   HSSP; P96110; 1B26.
DR   GeneID; 542220; -.
DR   Gramene; Q43260; -.
DR   MaizeGDB; 12238; -.
DR   BRENDA; 1.4.1.3; 289.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004353; F:glutamate dehydrogenase [NAD(P)+] activity; IEA:EC.
DR   GO; GO:0006520; P:amino acid metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR006095; Glu/Leu/Phe/Val_DH.
DR   InterPro; IPR006096; Glu/Leu/Phe/Val_DH_C.
DR   InterPro; IPR006097; Glu/Leu/Phe/Val_DH_dimer.
DR   InterPro; IPR014362; Glu_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11606:SF2; GLFV_DH; 1.
DR   Pfam; PF00208; ELFV_dehydrog; 1.
DR   Pfam; PF02812; ELFV_dehydrog_N; 1.
DR   PIRSF; PIRSF000185; Glu_DH; 1.
DR   PRINTS; PR00082; GLFDHDRGNASE.
DR   PROSITE; PS00074; GLFV_DEHYDROGENASE; 1.
PE   1: Evidence at protein level;
KW   NAD; Oxidoreductase.
FT   CHAIN         1    411       Glutamate dehydrogenase.
FT                                /FTId=PRO_0000182749.
FT   ACT_SITE    102    102       By similarity.
FT   VARIANT      18     18       L -> V (in strain: cv. P-10 Inbred).
FT   VARIANT     370    370       I -> M (in strain: cv. P-10 Inbred).
FT   VARIANT     376    376       N -> D (in strain: cv. P-10 Inbred).
FT   MUTAGEN     188    188       G->R: In GDH1.
FT   MUTAGEN     246    246       D->G: In GDH1.
FT   MUTAGEN     252    253       QL->EV: In GDH1.
FT   MUTAGEN     367    367       R->Q: In GDH1.
SQ   SEQUENCE   411 AA;  44022 MW;  648C67A9338922CF CRC64;
     MNALAATSRN FKQAAKLLGL DSKLEKSLLI PFREIKVECT IPKDDGTLAS YVGFRVQHDN
     ARGPMKGGIR YHHEVDPDEV NALAQLMTWK TAVANIPYGG AKGGIGCSPG DLSISELERL
     TRVFTQKIHD LIGIHTDVPA PDMGTNSQTM AWILDEYSKF HGYSPAVVTG KPVDLGGSLG
     RDAATGRGVL FATEALLAEH GKGIAGQRFV IQGFGNVGSW AAQLISEAGG KVIAISDVTG
     AVKNVDGLDI AQLVKHSAEN KGIKGFKGGD AIAPDSLLTE ECDVLIPAAL GGVINKDNAN
     DIKAKYIIEA ANHPTDPEAD EILSKKGVLI LPDILANSGG VTVSYFEWVQ NIQGFMWDEE
     KVNAELRTYI TRAFGNVKQM CRSHSCDLRM GAFTLGVNRV ARATVLRGWE A
//