Reviewed, UniProtKB/Swiss-Prot Q43260 (DHE3_MAIZE)

Names and origin

Protein names

Recommended name:
    Glutamate dehydrogenase
      Short name=GDH
    EC=1.4.1.3

Gene names

Name:

GDH1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Glutamate dehydrogenase		PRO_0000182749
Sites
	Active site	102	1	By similarity
Natural variations
	Natural variant	18	1	L → V in strain: cv. P-10 Inbred.
	Natural variant	370	1	I → M in strain: cv. P-10 Inbred.
	Natural variant	376	1	N → D in strain: cv. P-10 Inbred.
Experimental info
	Mutagenesis	188	1	G → R in GDH1.
	Mutagenesis	246	1	D → G in GDH1.
	Mutagenesis	252 – 253	2	QL → EV in GDH1.
	Mutagenesis	367	1	R → Q in GDH1.

Sequences

	Sequence		Length	Mass (Da)
	Q43260-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 648C67A9338922CF	FASTA	411	44,022
	10 20 30 40 50 60 MNALAATSRN FKQAAKLLGL DSKLEKSLLI PFREIKVECT IPKDDGTLAS YVGFRVQHDN 70 80 90 100 110 120 ARGPMKGGIR YHHEVDPDEV NALAQLMTWK TAVANIPYGG AKGGIGCSPG DLSISELERL 130 140 150 160 170 180 TRVFTQKIHD LIGIHTDVPA PDMGTNSQTM AWILDEYSKF HGYSPAVVTG KPVDLGGSLG 190 200 210 220 230 240 RDAATGRGVL FATEALLAEH GKGIAGQRFV IQGFGNVGSW AAQLISEAGG KVIAISDVTG 250 260 270 280 290 300 AVKNVDGLDI AQLVKHSAEN KGIKGFKGGD AIAPDSLLTE ECDVLIPAAL GGVINKDNAN 310 320 330 340 350 360 DIKAKYIIEA ANHPTDPEAD EILSKKGVLI LPDILANSGG VTVSYFEWVQ NIQGFMWDEE 370 380 390 400 410 KVNAELRTYI TRAFGNVKQM CRSHSCDLRM GAFTLGVNRV ARATVLRGWE A

References

[1]	"Isolation and characterization of a cDNA that encodes maize glutamate dehydrogenase." Sakakibara H., Fujii K., Sugiyama T. Plant Cell Physiol. 36:789-797(1995) [PubMed: 7551585] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Golden cross Bantam T51. Tissue: Root.
[2]	"Characterization of a GDH1-mutant in maize." Ju G.C. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTANT GDH1. Strain: cv. P-10 Inbred. Tissue: Shoot.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D49475 mRNA. Translation: BAA08445.1. U93560 mRNA. Translation: AAB51595.1. U93561 mRNA. Translation: AAB51596.1.
PIR	T03294. T04342.
RefSeq	NP_001105301.1.
UniGene	Zm.44
3D structure databases
HSSP	HSSP built from PDB template 1B26 based on UniProtKB P96110.
ModBase	Search...
Genome annotation databases
GeneID	542220.
Organism-specific databases
Gramene	Q43260.
MaizeGDB	12238.
Enzyme and pathway databases
BRENDA	1.4.1.3. 289.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE3_MAIZE

Accession

Primary (citable) accession number: Q43260
Secondary accession number(s): O04871, O04872

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	September 22, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)