Q43250 (C71C1_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxyindolin-2-one monooxygenase
EC=1.14.13.139

Alternative name(s):
Cytochrome P450 71C1
Protein benzoxazineless 4

Gene names

Name:	CYP71C1
Synonyms:	BX4

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of 3-hydroxyindolin-2-one to 2-hydroxy-1,4-benzoxazin-3-one (HBOA). Ref.2
Catalytic activity	3-hydroxyindolin-2-one + NAD(P)H + O2 = 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + NAD(P)+ + H2O. Ref.2 Ref.3
Cofactor	Heme group By similarity.
Pathway	Secondary metabolite biosynthesis; 2,4-dihydroxy-1,4-benzoxazin-3-one biosynthesis; 2,4-dihydroxy-1,4-benzoxazin-3-one from indoleglycerol phosphate: step 4/5.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding NAD NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	3-hydroxyindolin-2-one monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 535	535	3-hydroxyindolin-2-one monooxygenase		PRO_0000052113
Regions
	Transmembrane	14 – 34	21	Helical; Potential
	Transmembrane	469 – 489	21	Helical; Potential
Sites
	Metal binding	470	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	6	1	G → A in CAA57421. Ref.1
	Sequence conflict	138	1	S → C in CAA57421. Ref.1
	Sequence conflict	148	1	V → E in CAA57421. Ref.1
	Sequence conflict	268	1	S → G in CAA57421. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q43250 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 77ADF6889FD6FCF5	FASTA	535	59,716
	10 20 30 40 50 60 MALEAGYDYL HVAVVQCTPT QAAAVLGVLL LLAIRLAAAA RSSSATSPKW KQHRLPPTPP 70 80 90 100 110 120 GKLPIIGHLH LIGSHPHVSF RDLHAKYGHN GLMLVQVGAV PTIVVSTPQA AEAVLRTHDH 130 140 150 160 170 180 VLASRPRNPV ADIIRYNSTD VAFAPYGVYW RTARKVVNTH LLSAKMVFSK RREREEEVRL 190 200 210 220 230 240 VVARIRDAAE ASPGTALDMT ELLGGYASDF VCRAVLGESH RKQGRNKLFR ELTETSAALL 250 260 270 280 290 300 GGFNVEDYFP KLADVDLFLR IICAKAKSVS KRWDSLFNEL LSEYALSGGK QGDHNSEDFV 310 320 330 340 350 360 HLLLSLQKDY GLTTDNIKGI LVNMFEAAIE TSFLVLEYSM SELMNNRHVL AKLQKEVRTA 370 380 390 400 410 420 TPDGRMVMEE DLSRMPYLKA TIKESMRIHP PAPFLLPHFS THDCEINGYT IPAGTRVIVN 430 440 450 460 470 480 AWALARDPTC WDKAEEFFPE RFLEQGRDAE VDMYGKDIRF VPFGAGRRIC AGATFAIATV 490 500 510 520 530 EIMLANLIYH FDWEMPAEME RTGAKVDMSD QFGMTLRRTQ KLYLVPRIPK CVSSS

References

[1]	"Expression of a cytochrome P450 gene family in maize." Frey M., Kliem R., Saedler H., Gierl A. Mol. Gen. Genet. 246:100-109(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. CI31A.
[2]	"Analysis of a chemical plant defense mechanism in grasses." Frey M., Chomet P., Glawischnig E., Stettner C., Grun S., Winklmair A., Eisenreich W., Bacher A., Meeley R.B., Briggs S.P., Simcox K., Gierl A. Science 277:696-699(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.
[3]	"Studies on the biosynthesis of 2-hydroxy-1,4-benzoxazin-3-one (HBOA) from 3-hydroxyindolin-2-one in Zea mays." Spiteller P., Glawischnig E., Gierl A., Steglich W. Phytochemistry 57:373-376(2001) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, REACTION MECHANISM.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X81828 Genomic DNA. Translation: CAA57422.1. X81827 mRNA. Translation: CAA57421.1.
PIR	T03258.
UniGene	Zm.67282.
3D structure databases
ProteinModelPortal	Q43250.
ModBase	Search...
Proteomic databases
PRIDE	Q43250.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q43250.
MaizeGDB	114044.
Phylogenomic databases
HOGENOM	HOG000218629.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-10171.
UniPathway	UPA00872; UER00850.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C71C1_MAIZE

Accession

Primary (citable) accession number: Q43250
Secondary accession number(s): Q43254

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program