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Protein

Glyceraldehyde-3-phosphate dehydrogenase 3, cytosolic

Gene

GAPC3

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Essential for the maintenance of cellular ATP levels and carbohydrate metabolism (By similarity).By similarity

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase 1, cytosolic (GAPC1), Glyceraldehyde-3-phosphate dehydrogenase 3, cytosolic (GAPC3)
  2. no protein annotated in this organism
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase
  4. Enolase 1 (ENO1), Enolase 2 (ENO2)
  5. Pyruvate kinase, Pyruvate kinase (ZEAMMB73_471047), Pyruvate kinase, Pyruvate kinase, Pyruvate kinase, Pyruvate kinase, Pyruvate kinase, Pyruvate kinase (ZEAMMB73_227064), Pyruvate kinase (ZEAMMB73_409085), Pyruvate kinase, Pyruvate kinase, Pyruvate kinase, Pyruvate kinase, Pyruvate kinase, Pyruvate kinase (ZEAMMB73_438497), Pyruvate kinase, Pyruvate kinase (ZEAMMB73_571140), Pyruvate kinase (ZEAMMB73_227064), Pyruvate kinase, Pyruvate kinase, Pyruvate kinase, Pyruvate kinase
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35NADBy similarity1
Binding sitei82NAD; via carbonyl oxygenBy similarity1
Active sitei154NucleophilePROSITE-ProRule annotation1
Sitei181Activates thiol group during catalysisBy similarity1
Binding sitei184Glyceraldehyde 3-phosphateBy similarity1
Binding sitei236Glyceraldehyde 3-phosphateBy similarity1
Binding sitei318NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 14NADBy similarity2

GO - Molecular functioni

  • glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Source: AgBase
  • NAD+ binding Source: AgBase
  • NADP binding Source: InterPro
  • phosphate ion binding Source: AgBase

GO - Biological processi

  • glucose metabolic process Source: InterPro
  • glycolytic process Source: UniProtKB-UniPathway
  • response to anoxia Source: AgBase
  • response to heat Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 3, cytosolic (EC:1.2.1.12)
Gene namesi
Name:GAPC3
Synonyms:GPC3
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • protein complex Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001456071 – 337Glyceraldehyde-3-phosphate dehydrogenase 3, cytosolicAdd BLAST337

Proteomic databases

PaxDbiQ43247.
PRIDEiQ43247.

Expressioni

Gene expression databases

ExpressionAtlasiQ43247. baseline and differential.
GenevisibleiQ43247. ZM.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi4577.GRMZM2G071630_P01.

Structurei

3D structure databases

ProteinModelPortaliQ43247.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 151Binding to NADAdd BLAST151
Regioni152 – 337CatalyticAdd BLAST186
Regioni153 – 155Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni213 – 214Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
HOGENOMiHOG000071678.
KOiK00134.
OMAiISVFSER.
OrthoDBiEOG09360F5Y.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q43247-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKIKIGING FGRIGRLVAR VALQSDDVEL VAVNDPFIST DYMTYMFKYD
60 70 80 90 100
TVHGQWKHHE VKVKDSKTLL FGEKEVAVFG CRNPEEIPWG SVGAEYVVES
110 120 130 140 150
TGVFTDQEKA AAHLKGGAKK VVISAPSKDA PMFVVGVNEK EYKSDINIVS
160 170 180 190 200
NASCTTNCLA PLAKVINDKF GIVEGLMTTV HAITATQKTV DGPSSKDWRG
210 220 230 240 250
GRAASFNIIP SSTGAAKAVG KVLPVLNGKL TGMSFRVPTV DVSVVDLTVR
260 270 280 290 300
LEKSATYDEI KAAVKAEAEG SLKGILGYVE EDLVSTDFQG DSRSSIFDAK
310 320 330
AGIALNGNFV KLVSWYDNEW GYSTRVVDLI RHMNSTK
Length:337
Mass (Da):36,449
Last modified:November 1, 1996 - v1
Checksum:iB2C73B1961CA45EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U45856 mRNA. Translation: AAA87579.1.
L13431 mRNA. Translation: AAA33465.1.
PIRiPQ0179.
T02722.
RefSeqiNP_001105385.1. NM_001111915.2.
UniGeneiZm.28965.

Genome annotation databases

EnsemblPlantsiZm00001d051001_T001; Zm00001d051001_T001; Zm00001d051001.
Zm00001d051001_T002; Zm00001d051001_T002; Zm00001d051001.
GeneIDi542333.
GrameneiZm00001d051001_T001; Zm00001d051001_T001; Zm00001d051001.
Zm00001d051001_T002; Zm00001d051001_T002; Zm00001d051001.
KEGGizma:542333.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U45856 mRNA. Translation: AAA87579.1.
L13431 mRNA. Translation: AAA33465.1.
PIRiPQ0179.
T02722.
RefSeqiNP_001105385.1. NM_001111915.2.
UniGeneiZm.28965.

3D structure databases

ProteinModelPortaliQ43247.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4577.GRMZM2G071630_P01.

Proteomic databases

PaxDbiQ43247.
PRIDEiQ43247.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiZm00001d051001_T001; Zm00001d051001_T001; Zm00001d051001.
Zm00001d051001_T002; Zm00001d051001_T002; Zm00001d051001.
GeneIDi542333.
GrameneiZm00001d051001_T001; Zm00001d051001_T001; Zm00001d051001.
Zm00001d051001_T002; Zm00001d051001_T002; Zm00001d051001.
KEGGizma:542333.

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
HOGENOMiHOG000071678.
KOiK00134.
OMAiISVFSER.
OrthoDBiEOG09360F5Y.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Gene expression databases

ExpressionAtlasiQ43247. baseline and differential.
GenevisibleiQ43247. ZM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3PC3_MAIZE
AccessioniPrimary (citable) accession number: Q43247
Secondary accession number(s): Q06476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants contain two types of GAPDH: cytosolic forms which participate in glycolysis and chloroplast forms which participate in photosynthesis. All the forms are encoded by distinct genes.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.