ID   TCMO_ZINEL              Reviewed;         505 AA.
AC   Q43240;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   24-MAR-2009, entry version 51.
DE   RecName: Full=Trans-cinnamate 4-monooxygenase;
DE            EC=1.14.13.11;
DE   AltName: Full=Cinnamic acid 4-hydroxylase;
DE            Short=CA4H;
DE            Short=C4H;
DE   AltName: Full=P450C4H;
DE   AltName: Full=Cytochrome P450 73;
GN   Name=CYP73A12;
OS   Zinnia elegans (Zinnia).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC   Heliantheae; Zinnia.
OX   NCBI_TaxID=34245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Peter Pan;
RA   Ye Z.-H., Varner J.E.;
RT   "Expression patterns of cinnamic acid 4-hydroxylase gene during
RT   lignification in Zinnia elegans.";
RL   Plant Sci. 121:133-141(1996).
CC   -!- FUNCTION: Controls carbon flux to pigments essential for
CC       pollination or UV protection, to numerous pytoalexins synthesized
CC       by plants when challenged by pathogens, and to lignins.
CC   -!- CATALYTIC ACTIVITY: Trans-cinnamate + NADPH + O(2) = 4-
CC       hydroxycinnamate + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Phenylpropanoid metabolism; 4-coumaric acid biosynthesis;
CC       4-coumaric acid from trans-cinnamic acid: step 1/1.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; U19922; AAB42024.1; -; mRNA.
DR   BRENDA; 1.14.13.11; 228834.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0016710; F:trans-cinnamate 4-monooxygenase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Metal-binding; Monooxygenase; NADP; Oxidoreductase.
FT   CHAIN         1    505       Trans-cinnamate 4-monooxygenase.
FT                                /FTId=PRO_0000052255.
FT   METAL       447    447       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   505 AA;  57914 MW;  C51AAAEBD36C55C8 CRC64;
     MDLLLVEKTL LALFAAIIAS IFISKLRGKR FKLPPGPVPV PIFGNWLQVG DDLNHRNLTD
     LAKKFGEIFL LRMGQRNLVV VSSPNLAKEV LHTQGVEFGS RTRNVVFDIF TGKGQDMVFT
     VYGEHWRKMR RIMTVPFFTN KVVQQYRTGW EAEAAAVVDD VKKNPKAATE GVVIRKRLQL
     MMYNNMFRIM FDRRFESEDD PLFVKLKMLN GERSRLAQSF EYNYGDFIPI LRPFLKGYLK
     LCKEVKEKRF QLFKDYFVDE RKKLGSTKSM DNNQLKCAID HILDAKDKGE INEDNVLYIV
     ENINVAAIET TLWSIEWAIA ELVNHPEIQA KLRHELVSQL GPGVQVTEPD LHKLPYLQAV
     IKETLRLRMA IPLLVPHMNL HDAKLGGYDI PAESKILVNA WWLANNPDQW KKPEEFRPER
     FLEEESKVEA NGNDFRYLPF GVGRRSCPGI ILALPILGIT IGRLVQNFEL LPPPGQDKVD
     TTEKGGQFSL HILKHSTIVA KPRVL
//