Reviewed, UniProtKB/Swiss-Prot Q43240 (TCMO_ZINEL)

Names and origin

Protein names

Recommended name:
    Trans-cinnamate 4-monooxygenase
    EC=1.14.13.11
Alternative name(s):
    Cinnamic acid 4-hydroxylase
      Short name=CA4H
      Short name=C4H
    P450C4H
    Cytochrome P450 73

Gene names

Name:

CYP73A12

Organism

Zinnia elegans (Zinnia)

Taxonomic identifier

34245 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Heliantheae › Zinnia

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.
Catalytic activity	Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Phenylpropanoid metabolism; trans-4-coumarate biosynthesis; trans-4-coumarate from trans-cinnamate: step 1/1.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro trans-cinnamate 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Trans-cinnamate 4-monooxygenase		PRO_0000052255
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43240-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C51AAAEBD36C55C8	FASTA	505	57,914
	10 20 30 40 50 60 MDLLLVEKTL LALFAAIIAS IFISKLRGKR FKLPPGPVPV PIFGNWLQVG DDLNHRNLTD 70 80 90 100 110 120 LAKKFGEIFL LRMGQRNLVV VSSPNLAKEV LHTQGVEFGS RTRNVVFDIF TGKGQDMVFT 130 140 150 160 170 180 VYGEHWRKMR RIMTVPFFTN KVVQQYRTGW EAEAAAVVDD VKKNPKAATE GVVIRKRLQL 190 200 210 220 230 240 MMYNNMFRIM FDRRFESEDD PLFVKLKMLN GERSRLAQSF EYNYGDFIPI LRPFLKGYLK 250 260 270 280 290 300 LCKEVKEKRF QLFKDYFVDE RKKLGSTKSM DNNQLKCAID HILDAKDKGE INEDNVLYIV 310 320 330 340 350 360 ENINVAAIET TLWSIEWAIA ELVNHPEIQA KLRHELVSQL GPGVQVTEPD LHKLPYLQAV 370 380 390 400 410 420 IKETLRLRMA IPLLVPHMNL HDAKLGGYDI PAESKILVNA WWLANNPDQW KKPEEFRPER 430 440 450 460 470 480 FLEEESKVEA NGNDFRYLPF GVGRRSCPGI ILALPILGIT IGRLVQNFEL LPPPGQDKVD 490 500 TTEKGGQFSL HILKHSTIVA KPRVL

References

[1]	"Expression patterns of cinnamic acid 4-hydroxylase gene during lignification in Zinnia elegans." Ye Z.-H., Varner J.E. Plant Sci. 121:133-141(1996) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Peter Pan.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U19922 mRNA. Translation: AAB42024.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.11. 228834.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCMO_ZINEL

Accession

Primary (citable) accession number: Q43240

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	September 22, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)