ID   PALY_WHEAT              Reviewed;         700 AA.
AC   Q43210;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 61.
DE   RecName: Full=Phenylalanine ammonia-lyase;
DE            EC=4.3.1.24;
GN   Name=PAL;
OS   Triticum aestivum (Wheat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade;
OC   Pooideae; Triticeae; Triticum.
OX   NCBI_TaxID=4565;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Chinese Spring;
RA   Liao Y.-C., Li H.-P., Kreuzaler F., Fischer R.;
RT   "Nucleotide sequence of one of two tandem genes encoding phenylalanine
RT   ammonia-lyase in Triticum aestivum.";
RL   (er) Plant Gene Register PGR96-102.
CC   -!- FUNCTION: This is a key enzyme of plant metabolism catalyzing the
CC       first reaction in the biosynthesis from L-phenylalanine of a wide
CC       variety of natural products based on the phenylpropane skeleton.
CC   -!- CATALYTIC ACTIVITY: L-phenylalanine = trans-cinnamate + ammonia.
CC   -!- PATHWAY: Phenylpropanoid metabolism; cinnamic acid biosynthesis;
CC       trans-cinnamic acid from L-phenylalanine: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- PTM: Contains an active site 4-methylidene-imidazol-5-one (MIO),
CC       which is formed autocatalytically by cyclization and dehydration
CC       of residues Ala-Ser-Gly (By similarity).
CC   -!- SIMILARITY: Belongs to the PAL/histidase family.
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DR   EMBL; X99705; CAA68036.1; -; Genomic_DNA.
DR   PIR; T06545; T06545.
DR   HSSP; P21310; 1GKM.
DR   SMR; Q43210; 12-700.
DR   Gramene; Q43210; -.
DR   BRENDA; 4.3.1.5; 253.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016211; F:ammonia ligase activity; IEA:InterPro.
DR   GO; GO:0016841; F:ammonia-lyase activity; IEA:InterPro.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   GO; GO:0006559; P:L-phenylalanine catabolic process; IEA:InterPro.
DR   GO; GO:0009698; P:phenylpropanoid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001106; Phe/His_NH3-lyase.
DR   InterPro; IPR005922; Phe_NH3-lyase.
DR   Pfam; PF00221; PAL; 1.
DR   TIGRFAMs; TIGR01226; phe_am_lyase; 1.
DR   PROSITE; PS00488; PAL_HISTIDASE; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Lyase; Phenylpropanoid metabolism.
FT   CHAIN         1    700       Phenylalanine ammonia-lyase.
FT                                /FTId=PRO_0000215428.
FT   MOD_RES     187    187       2,3-didehydroalanine (Ser) (By
FT                                similarity).
FT   CROSSLNK    186    188       5-imidazolinone (Ala-Gly) (By
FT                                similarity).
SQ   SEQUENCE   700 AA;  75938 MW;  56EE37B4E74E20B5 CRC64;
     MACAWRSRSR ADPLNWGKAA EELSGSHLEA VKRMVEEYRK PVVTMEGATT IAMVAAVAAG
     SDTRVELDES ARGRVKESSD WVMNSMMNGT DSYGVTTGFG ATSHRRTKEG GALQRELIRF
     LNAGAFGTGT DGHVLPAAAT RAAMLVRVNT LLQGYSGIRF EILETIATLL NANVTPCLPL
     RGTITASGDL VPLSYIAGLV TGRPNSMATA PDGSKVNAAE AFKIAGIQHG FFELQPKEGL
     AMVNGTAVGS GLASMVLFEA NVLSLLAEVL SGVFCEVMNG KPEFTDHLTH KLKHHPGQIE
     AAAIMEHILE GSSYMMLAKK LGELDPLMKP KQDRYALRTS PQWLGPQIEV IRAATKSIER
     EINSVNDNPL IDVSRGKAIH GGNFQGTPIG VSMDNTRLAI AAIGKLMFAQ FSELVNDFYN
     NGLPSNLSGG RNPSLDYGFK GAEIAMASYC SELQFLGNPV TNHVQSAEQH NQDVNSLGLI
     SSRKTAEAID ILKLMSSTFL VALCQAIDLR HLEENVKNAV KSCVKTVARK TLSTDNNGHL
     HNARFCEKDL LLTIDREAVF AYADDPCSAN YPLMQKMRAV LVEHALANGE AEAHVETSVF
     AKLAMFEQEL RAVLPKEVEA ARSAVENGTA AQQNRIAECR SYPLYRFVRK ELGTEYLTGE
     KTRSPGEEVD KVFVAMNQGK HIDALLECLK EWNGEPLPLC
//