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Protein

Probable linoleate 9S-lipoxygenase 5

Gene

LOX1.5

Organism
Solanum tuberosum (Potato)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. May contribute to cell death during the hypersensitive response (HR) by the massive production of free fatty acid hydroperoxides. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Catalytic activityi

Linoleate + O2 = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.PROSITE-ProRule annotation

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi523 – 5231Iron; catalyticPROSITE-ProRule annotation
Metal bindingi528 – 5281Iron; catalyticPROSITE-ProRule annotation
Metal bindingi714 – 7141Iron; catalyticPROSITE-ProRule annotation
Metal bindingi718 – 7181Iron; catalyticPROSITE-ProRule annotation
Metal bindingi862 – 8621Iron; via carboxylate; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable linoleate 9S-lipoxygenase 5 (EC:1.13.11.58)
Alternative name(s):
Leaf lipoxygenase
Gene namesi
Name:LOX1.5
Synonyms:POTLX-3
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
Proteomesi
  • UP000011115 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2189149.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 862862Probable linoleate 9S-lipoxygenase 5PRO_0000412923Add
BLAST

Expressioni

Tissue specificityi

Not detected in leaves, stems, flowers, roots, tubers and stolons during normal growth and development.1 Publication

Inductioni

Up-regulated within 6 hours after pathogen infection, ethylene or jasmonate treatments. Not induced by wounding.1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi4113.PGSC0003DMT400028158.

Chemistry

BindingDBiQ43191.

Structurei

3D structure databases

ProteinModelPortaliQ43191.
SMRiQ43191. Positions 18-862.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 161126PLATPROSITE-ProRule annotationAdd
BLAST
Domaini164 – 862699LipoxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IH0D. Eukaryota.
ENOG410YN4N. LUCA.
InParanoidiQ43191.
KOiK15718.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 2 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q43191-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLEKIVEAI SGRSEDNGKK VKGTIVLMKK NVLDFNDVNA SLLDGVLEFL
60 70 80 90 100
GKRVSLQLIS VVHADPGNSL QGKRSNPAYL EKWLTTGTSL VAGESAFDVT
110 120 130 140 150
FDWDEDIGVP GAFIINNFHF NEFYLKSLTL EDVPNHGNVH FVCNSWVYPA
160 170 180 190 200
KKYKSERIFF ANQAYLPGET PEPLRNYREK ELVNLRGNGN GKLEEWDRVY
210 220 230 240 250
DYALYNDLGD PEKGKQYART ILGGSAEYPY PRRGRTGRKP TKADPKSESR
260 270 280 290 300
IPLLMSLDIY VPRDERFGHI KLSDFLTYAL KSIVQFLIPE FQALFDSTPD
310 320 330 340 350
EFDSFEDVLK LYEGGIKLPQ GPFLKALTDS IPLEILKEII RTDGEGKFKF
360 370 380 390 400
PTPQVIQEDK SSWRTDEEFA REMLAGVNPV IISRLQEFPP KSQLDSEVYG
410 420 430 440 450
NQNSTITKEH IENTLDGLTI DDAIKTNRLY ILNHHDILMP YVRRINTTNT
460 470 480 490 500
KLYASRTLLF LQDDGTMKPV AIELSLPHPD GDELGAVSKV YTPADQGVEG
510 520 530 540 550
SIWQLAKAYV AVNDSGVHQL ISHWLNTHAA IEPFVIATNR QLSVLHPIHK
560 570 580 590 600
LLHPHFRDTM NINALARQIL INAGGVLEMT VFPAKYAMEM SAVVYKSWVF
610 620 630 640 650
PEQALPADLI KRGVAVEDSS SPHGVRLLIQ DYPYAVDGLE IWSAIKSWVT
660 670 680 690 700
EYCNFYYKSD ELVLKDNELQ AWWKELREEG HGDKKDEPWW PKMQTRQELK
710 720 730 740 750
DSCTIIIWIA SALHAAVNFG QYPYAGYLPN RPTLSRRFMP EPGTPEYEEL
760 770 780 790 800
KTNPDKAYLK TITPQLQTLL GISLIEILSR HASDEIYLGQ RDSSEWTKDQ
810 820 830 840 850
EPIAAFERFG KKLSEIEDQI IQMNGDKKWK NRSGPVNVPY TLLFPTSEQG
860
LTGKGIPNSV SI
Length:862
Mass (Da):97,778
Last modified:November 1, 1996 - v1
Checksum:i94667A9F56041E89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60202 mRNA. Translation: AAB67865.1.
PIRiT07775.
RefSeqiNP_001274916.1. NM_001287987.1.
UniGeneiStu.18586.

Genome annotation databases

GeneIDi102577714.
KEGGisot:102577714.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60202 mRNA. Translation: AAB67865.1.
PIRiT07775.
RefSeqiNP_001274916.1. NM_001287987.1.
UniGeneiStu.18586.

3D structure databases

ProteinModelPortaliQ43191.
SMRiQ43191. Positions 18-862.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4113.PGSC0003DMT400028158.

Chemistry

BindingDBiQ43191.
ChEMBLiCHEMBL2189149.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi102577714.
KEGGisot:102577714.

Phylogenomic databases

eggNOGiENOG410IH0D. Eukaryota.
ENOG410YN4N. LUCA.
InParanoidiQ43191.
KOiK15718.

Enzyme and pathway databases

UniPathwayiUPA00382.

Miscellaneous databases

PROiQ43191.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 2 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of a cDNA clone for a lipoxygenase from abscisic acid-treated potato leaves."
    Kolomiets M.V., Hannapel D.J., Gladon R.J.
    Plant Gene Register PGR96-069
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Berolina.
  2. "A leaf lipoxygenase of potato induced specifically by pathogen infection."
    Kolomiets M.V., Chen H., Gladon R.J., Braun E.J., Hannapel D.J.
    Plant Physiol. 124:1121-1130(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY PATHOGEN; ETHYLENE AND JASMONATE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiLOX15_SOLTU
AccessioniPrimary (citable) accession number: Q43191
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.