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Q43157

- RPE_SPIOL

UniProt

Q43157 - RPE_SPIOL

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Protein

Ribulose-phosphate 3-epimerase, chloroplastic

Gene

RPE

Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.3 Publications

Catalytic activityi

D-ribulose 5-phosphate = D-xylulose 5-phosphate.3 Publications

Cofactori

Binds 1 divalent metal cation per subunit. Active with Co2+, Fe2+, Mn2+ and Zn2+ By similarity.By similarity

Kineticsi

  1. KM=250 µM for ribulose 5-phosphate2 Publications

pH dependencei

Optimum pH is 8.5.2 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei67 – 671SubstrateBy similarity
Metal bindingi92 – 921Divalent metal cationBy similarity
Active sitei94 – 941Proton acceptorBy similarity
Metal bindingi94 – 941Divalent metal cationBy similarity
Metal bindingi125 – 1251Divalent metal cationBy similarity
Binding sitei125 – 1251SubstrateBy similarity
Active sitei236 – 2361Proton donor1 Publication
Metal bindingi236 – 2361Divalent metal cationBy similarity
Binding sitei238 – 2381Substrate; via amide nitrogenBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribulose-phosphate 3-epimerase activity Source: UniProtKB

GO - Biological processi

  1. pentose-phosphate shunt Source: UniProtKB
  2. reductive pentose-phosphate cycle Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Calvin cycle, Carbohydrate metabolism, Pentose shunt

Keywords - Ligandi

Cobalt, Iron, Manganese, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose-phosphate 3-epimerase, chloroplastic (EC:5.1.3.1)
Alternative name(s):
Pentose-5-phosphate 3-epimerase
Short name:
PPE
R5P3E
Short name:
RPE
Gene namesi
Name:RPE
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

Plastidchloroplast thylakoid membrane 1 Publication

GO - Cellular componenti

  1. chloroplast thylakoid membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi236 – 2361D → A, E or N: Almost abolished enzyme activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5050Chloroplast1 PublicationAdd
BLAST
Chaini51 – 285235Ribulose-phosphate 3-epimerase, chloroplasticPRO_0000025416Add
BLAST

Proteomic databases

PRIDEiQ43157.

Interactioni

Subunit structurei

Homooctamer.2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ43157.
SMRiQ43157. Positions 52-281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni203 – 2064Substrate bindingBy similarity
Regioni258 – 2592Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000056. Ribul_P_3_epim-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PANTHERiPTHR11749. PTHR11749. 1 hit.
PfamiPF00834. Ribul_P_3_epim. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01163. rpe. 1 hit.
PROSITEiPS01085. RIBUL_P_3_EPIMER_1. 1 hit.
PS01086. RIBUL_P_3_EPIMER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43157-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAASLCQST LQSQINGFCG GLNIRKLQPS TSSPNSLTFT RRKVQTLVKA
60 70 80 90 100
TSRVDKFSKS DIIVSPSILS ANFAKLGEQV KAVELAGCDW IHVDVMDGRF
110 120 130 140 150
VPNITIGPLV VDALRPVTDL PLDVHLMIVE PEQRVPDFIK AGADIVSVHC
160 170 180 190 200
ELASTIHLHR TVNQIKSLGA KAGVVLNPGT PLSTIEYVLD VVDLVLIMSV
210 220 230 240 250
NPGFGGQSFI ESQVKKISDL RKMCVEKGVN PWIEVDGGVT PANAYKVIEA
260 270 280
GANALVAGSA VFGAKDYAEA IKGIKASKRP EPVAV
Length:285
Mass (Da):30,366
Last modified:November 1, 1996 - v1
Checksum:iED0236FB7B629512
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42328 mRNA. Translation: AAC41677.1.
AF070941 mRNA. Translation: AAC24708.1.
PIRiS62724.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42328 mRNA. Translation: AAC41677.1 .
AF070941 mRNA. Translation: AAC24708.1 .
PIRi S62724.

3D structure databases

ProteinModelPortali Q43157.
SMRi Q43157. Positions 52-281.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q43157.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00116 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR000056. Ribul_P_3_epim-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view ]
PANTHERi PTHR11749. PTHR11749. 1 hit.
Pfami PF00834. Ribul_P_3_epim. 1 hit.
[Graphical view ]
SUPFAMi SSF51366. SSF51366. 1 hit.
TIGRFAMsi TIGR01163. rpe. 1 hit.
PROSITEi PS01085. RIBUL_P_3_EPIMER_1. 1 hit.
PS01086. RIBUL_P_3_EPIMER_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of the amphibolic Calvin cycle/OPPP enzyme D-ribulose-5-phosphate 3-epimerase (EC 5.1.3.1) from spinach chloroplasts: functional and evolutionary aspects."
    Nowitzki U., Wyrich R., Westhoff P., Henze K., Schnarrenberger C., Martin W.F.
    Plant Mol. Biol. 29:1279-1291(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
  2. "D-ribulose-5-phosphate 3-epimerase: cloning and heterologous expression of the spinach gene, and purification and characterization of the recombinant enzyme."
    Chen Y.-R., Hartman F.C., Lu T.-Y.S., Larimer F.W.
    Plant Physiol. 118:199-207(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
    Strain: cv. Melody.
  3. "Purification, properties and in situ localization of the amphibolic enzymes D-ribulose 5-phosphate 3-epimerase and transketolase from spinach chloroplasts."
    Teige M., Melzer M., Suess K.-H.
    Eur. J. Biochem. 252:237-244(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 51-66, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
    Strain: cv. Matador.
  4. "Identification of a catalytic aspartyl residue of D-ribulose 5-phosphate 3-epimerase by site-directed mutagenesis."
    Chen Y.-R., Larimer F.W., Serpersu E.H., Hartman F.C.
    J. Biol. Chem. 274:2132-2136(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, ACTIVE SITE, MUTAGENESIS OF ASP-236.

Entry informationi

Entry nameiRPE_SPIOL
AccessioniPrimary (citable) accession number: Q43157
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 1, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3