Reviewed, UniProtKB/Swiss-Prot Q43155 (GLTB_SPIOL)

Names and origin

Protein names	Recommended name:     Ferredoxin-dependent glutamate synthase, chloroplastic     EC=1.4.7.1 Alternative name(s):     Fd-GOGAT
Organism	Spinacia oleracea (Spinach)
Taxonomic identifier	3562 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	1517 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+.
Cofactor	Binds 1 3Fe-4S cluster. FAD. FMN.
Pathway	Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (ferredoxin route): step 1/1. Energy metabolism; nitrogen metabolism.
Subcellular location	Plastid › chloroplast stroma.
Tissue specificity	Leaves.
Sequence similarities	Belongs to the glutamate synthase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Glutamate biosynthesis
Cellular component	Chloroplast Plastid
Domain	Glutamine amidotransferase
Ligand	3Fe-4S FAD FMN Flavoprotein Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate synthase (ferredoxin) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1517	1517	Ferredoxin-dependent glutamate synthase, chloroplastic		PRO_0000170796
Regions
	Domain	1 – 400	400	Glutamine amidotransferase type-2
	Nucleotide binding	1079 – 1136	58	FMN By similarity
Sites
	Active site	1	1	For GATase activity By similarity
	Metal binding	1132	1	Iron-sulfur (3Fe-4S) By similarity
	Metal binding	1138	1	Iron-sulfur (3Fe-4S) By similarity
	Metal binding	1143	1	Iron-sulfur (3Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43155-1 [UniParc]. Last modified January 11, 2001. Version 3. Checksum: 2304650E1F9350A6	FASTA	1,517	165,402
	10 20 30 40 50 60 CGVGFIANLD NKGSFQIVKD ALTALGCMEH RGGCGSDNDS GDGSGVMTAI PWDLFNDWGK 70 80 90 100 110 120 DQGIGPFDRS HTGVGMVFLP KDDLLAEEAK KVVLDTFAQE GIEVIGWRSV PTNVSVVGRN 130 140 150 160 170 180 AKETMPNIQQ VFVRIIKEDS TDDIERELYI CRKLIERAAS SHTWASELYF CSLSNQTIIY 190 200 210 220 230 240 KGMLRSEVLG MFYYDLQNER YTSPFAIYHR RYSTNTSPRW PLAQPMRFLG HNGEINTIQG 250 260 270 280 290 300 NLNWMRSREP SIQSPVWRGR ENEIRPYGNP KASDSANLDS AAELLIRSGR TPEEALMILV 310 320 330 340 350 360 PEAYKNHPTL MIKYPEAVDF YDYYKGQMET WDGPALLLFS DGKTVGACLD RNGLAPARYW 370 380 390 400 410 420 RTVDNVVYVA SEVGVLPMDE SKVTMKGRLG PGMMISVDLS SGQVYENTEV KKRVASSNPY 430 440 450 460 470 480 GKWVKENLRS LKAVNFLSRA LLENDTILRN QQAFGYSSED VQMVIESMAS QGKEPTFCMG 490 500 510 520 530 540 DDIPLAVMSQ KPHMLYDYFK QRFAQVTNPA IDPLREGLVM SLEVNIGKRG NILEVGPENA 550 560 570 580 590 600 SQVILPSPVL NEGELEALVN DPLLKAQMLP IFFDIRKGVE GTLEKRLNRL CEAADEAVRN 610 620 630 640 650 660 GSQMLVLSDR SEELEPTRPA IPILLAVGAV HQHLIQNGLR MYTSIVVDTA QCFSTHQFAC 670 680 690 700 710 720 LIGYGASAIC PYLALETCRQ WRLSNKTVNL MRTGKIPTVT IEQAQNNFCK AVKSGLLKIL 730 740 750 760 770 780 SKMGISLLSS YCGAQIFEIY GLGKDVVDIA FQGSVSKMGG LTLDELARET LSFWVKAFSE 790 800 810 820 830 840 DTAKRLENFG FIQFRPGGEY HVNNPEMSKL LHKAVRNKSE SAYAVYQQHL ANRPVSVLRD 850 860 870 880 890 900 LLEFKSDRAP ISVGKVEPAT SIVERFCTGG MSLGAISRET HEAIAIAMNR LGGKSNSGEG 910 920 930 940 950 960 GEDPIRWRPL TDVVDGYSST LPHLKGLQNG DTATSAIKQV ASGRFGVTPT FLVNADQIEI 970 980 990 1000 1010 1020 KIAQGAKPGE GGQLPGKKVS AYIARLRNSK PGVPLISPPP HHDIYSIEDL AQLIYDLHQI 1030 1040 1050 1060 1070 1080 NPKAKVSVKL VAEAGIGTVA SGVAKGNADI IQVSGHDGGT GASPISSIKH AGGPWELGLS 1090 1100 1110 1120 1130 1140 ETHQTLISNG LRERVILRVD GGLKCGVDVM MAAAMGADEY GFGSLAMIAT GCVMARICHT 1150 1160 1170 1180 1190 1200 NNCPVGVASQ REELRARFPG VPGDLVNFFL YVAEEVRGIL AQLGFEKLDD IIGRTDILKP 1210 1220 1230 1240 1250 1260 RDISLMKTQH LDLSYILASA GLPTMSSTAI RKQEVHTNGP VLDDQILSDP EIIDAIENEK 1270 1280 1290 1300 1310 1320 IVNKTVKIFN VDRAVCGRIA GVIAKKYGDT GFAGQLNLTF EGSAGQSFAV FLTPGMNIRL 1330 1340 1350 1360 1370 1380 VGESNDYVGK GMAGGELIVT PAENPGFRPE DATIVGNTCL YGATGGQIFV RGKAGERFAV 1390 1400 1410 1420 1430 1440 RNSLAEAVVE GTGDHCCEYM TGGCVVILGK VGRNVAAGMT GGLAYILDED DTLIPKVNKE 1450 1460 1470 1480 1490 1500 IVKIQRVTAP VGQMQLKNLI EAHVEKTGSS KGASILKDWD KYLPLFWQLV PPSEEDTPEA 1510 SAMFEQMTSE GASLQSA

References

[1]	Dincturk H.B., Knaff D.B. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Melody. Tissue: Leaf.
[2]	"Cloning and sequencing of the gene encoding spinach ferredoxin-dependent glutamate synthase." Nalbantoglu B., Hirasawa M., Moomaw C., Nguyen H., Knaff D.B., Allen R. Biochim. Biophys. Acta 1183:557-561(1994) [PubMed: 8286406] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-1517, PROTEIN SEQUENCE OF 1-24. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF061515 mRNA. Translation: AAC26853.1. U03006 mRNA. Translation: AAA18948.1.
PIR	S67496.
3D structure databases
HSSP	HSSP built from PDB template 1OFD based on UniProtKB P55038.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.7.1. 286.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000583. GATase_2. IPR017932. GATase_II. IPR002932. Glu_synth_centr_C. IPR006982. Glu_synth_centr_N. IPR002489. Glu_synthase_C. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 2 hits. G3DSA:2.160.20.60. Glu_synthase_C. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF04898. Glu_syn_central. 1 hit. PF01645. Glu_synthase. 1 hit. PF01493. GXGXG. 1 hit. [Graphical view]
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLTB_SPIOL

Accession

Primary (citable) accession number: Q43155
Secondary accession number(s): O81234

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 11, 2001
Last modified:	June 16, 2009
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)