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Q43154 (GSHRP_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione reductase, chloroplastic

Short name=GR
Short name=GRase
EC=1.8.1.7
OrganismSpinacia oleracea (Spinach)
Taxonomic identifier3562 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Protein attributes

Sequence length489 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Maintains high levels of reduced glutathione in the chloroplast.

Catalytic activity

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactor

Binds 1 FAD per subunit By similarity.

Subcellular location

Plastidchloroplast.

Miscellaneous

The active site is a redox-active disulfide bond.

Sequence similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide‹1 – 2›2Chloroplast Potential
Chain3 – 489487Glutathione reductase, chloroplastic
PRO_0000030283

Regions

Nucleotide binding54 – 6310FAD By similarity

Sites

Active site4621Proton acceptor By similarity
Binding site2241NADP By similarity
Binding site2301NADP By similarity

Amino acid modifications

Disulfide bond63 ↔ 68Redox-active By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Q43154 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 970FC5B079A72CCD

FASTA48952,611
        10         20         30         40         50         60 
EDNHSTVEDD AANYDFDLFV IGAGSGGVRA ARFSANLGAK VGICELPFHP ISSEVIGGVG 

        70         80         90        100        110        120 
GTCVIRGCVP KKILVYGASF GGELEDAKNY GWELNEKIDF NWKKLLQKKT DEIIRLNNIY 

       130        140        150        160        170        180 
KRLLSNAGVK LYEGEGKIVG PNEVQVTQLD GTKLSYSAKH ILIATGSRAQ RPNIPGQELA 

       190        200        210        220        230        240 
ITSDEALSLE EFPKRVVILG GGYISVEFAS IWRGMGADVN LCFRKELPLR GFDDEMRAAV 

       250        260        270        280        290        300 
ARNLEGRGVN VHPRTTLTEL VKTDGGVVAR TDHGEEIEAD VVLFATGRSP NTKRLNLEAL 

       310        320        330        340        350        360 
GVELDRTGAV KVDEYSRTSV PSIWAIGDVT NRMNLTPVAL MEGTCFAKTV FGGQNSKPDY 

       370        380        390        400        410        420 
SNIACAVFSI PPLAVVGLSE EQAIEQASGD ILVFTSSFNP MKNTISGRQE KTIMKLVVDA 

       430        440        450        460        470        480 
ETDKVLGASM CGPDAAEIMQ GIAIALKFGA TKAQFDSTVG IHPSAAEEFV TMREPSRRVP 


GAGKPKTNL 

« Hide

References

[1]Aono M., Fujiyama K., Sano T., Tanaka K.
Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. New Asia.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D37870 mRNA. Translation: BAA07108.1.
PIRT09151.

3D structure databases

ProteinModelPortalQ43154.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.390.30. 1 hit.
InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006324. Glut-diS_reduct.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. SSF55424. 1 hit.
TIGRFAMsTIGR01424. gluta_reduc_2. 1 hit.
PROSITEPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHRP_SPIOL
AccessionPrimary (citable) accession number: Q43154
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families