Q43143 (PMEU1_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pectinesterase/pectinesterase inhibitor U1

Including the following 2 domains:

1. Pectinesterase inhibitor U1
   Alternative name(s):
   Pectin methylesterase inhibitor U1
2. Pectinesterase U1
   Short name=PE U1
   EC=3.1.1.11
   Alternative name(s):
   Pectin methylesterase U1

Gene names

Name:

PMEU1

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted › cell wall Probable.
Miscellaneous	The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.
Sequence similarities	In the N-terminal section; belongs to the PMEI family. In the C-terminal section; belongs to the pectinesterase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW enzyme inhibitor activity Inferred from electronic annotation. Source: InterPro pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 40	40	Potential
	Chain	41 – 583	543	Pectinesterase/pectinesterase inhibitor U1		PRO_0000023493
Regions
	Region	60 – 221	162	Pectinesterase inhibitor U1
	Region	272 – 570	299	Pectinesterase U1
Sites
	Active site	400	1	Proton donor; for pectinesterase activity By similarity
	Active site	421	1	Nucleophile; for pectinesterase activity By similarity
	Binding site	347	1	Substrate; for pectinesterase activity By similarity
	Binding site	377	1	Substrate; for pectinesterase activity By similarity
	Binding site	489	1	Substrate; for pectinesterase activity By similarity
	Binding site	491	1	Substrate; for pectinesterase activity By similarity
	Site	399	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	105	1	N-linked (GlcNAc...) Potential
	Glycosylation	224	1	N-linked (GlcNAc...) Potential
	Disulfide bond	414 ↔ 434		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43143 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FA7434833CDA9EF2	FASTA	583	64,103
	10 20 30 40 50 60 MTRVEDFFSK QIDFCKRKKK IYLAIVASVL LVAAVIGVVA GVKSHSKNSD DHADIMAISS 70 80 90 100 110 120 SAHAIVKSAC SNTLHPELCY SAIVNVSDFS KKVTSQKDVI ELSLNITVKA VRRNYYAVKE 130 140 150 160 170 180 LIKTRKGLTP REKVALHDCL ETMDETLDEL HTAVEDLELY PNKKSLKEHV EDLKTLISSA 190 200 210 220 230 240 ITNQETCLDG FSHDEADKKV RKVLLKGQKH VEKMCSNALA MICNMTDTDI ANEMKLSAPA 250 260 270 280 290 300 NNRKLVEDNG EWPEWLSAGD RRLLQSSTVT PDVVVAADGS GDYKTVSEAV RKAPEKSSKR 310 320 330 340 350 360 YVIRIKAGVY RENVDVPKKK TNIMFMGDGK SNTIITASRN VQDGSTTFHS ATVVRVAGKV 370 380 390 400 410 420 LARDITFQNT AGASKHQAVA LCVGSDLSAF YRCDMLAYQD TLYVHSNRQF FVQCLVAGTV 430 440 450 460 470 480 DFIFGNGAAV FQDCDIHARR PGSGQKNMVT AQGRTDPNQN TGIVIQKCRI GATSDLRPVQ 490 500 510 520 530 540 KSFPTYLGRP WKEYSRTVIM QSSITDVIQP AGWHEWNGNF ALDTLFYGEY ANTGAGAPTS 550 560 570 580 GRVKWKGHKV ITSSTEAQAY TPGRFIAGGS WLSSTGFPFS LGL

References

[1]	"Cloning and nucleotide sequence of a pectin methylesterase cDNA homologue from tomato leaves." Gaffe J., Tiznado M.E., Handa A.K. Plant Gene Register PGR96-017 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Rio Grande. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U49330 mRNA. Translation: AAD09283.1.
PIR	T07848.
RefSeq	NP_001233857.1. NM_001246928.1.
UniGene	Les.67.
3D structure databases
ProteinModelPortal	Q43143.
SMR	Q43143. Positions 265-583.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	544016.
Enzyme and pathway databases
BRENDA	3.1.1.11. 3101.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. IPR006501. Pectinesterase_inhib. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit. G3DSA:1.20.140.40. Pectinesterase_inhib. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. PF04043. PMEI. 1 hit. [Graphical view]
SMART	SM00856. PMEI. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit. SSF101148. Pectinesterase_inhib. 1 hit.
TIGRFAMs	TIGR01614. PME_inhib. 1 hit.
PROSITE	PS00800. PECTINESTERASE_1. 1 hit. PS00503. PECTINESTERASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMEU1_SOLLC

Accession

Primary (citable) accession number: Q43143

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program