ID   F16P2_SACHY             Reviewed;         343 AA.
AC   Q43139;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 2.
DT   16-JUN-2009, entry version 52.
DE   RecName: Full=Fructose-1,6-bisphosphatase, cytosolic;
DE            Short=FBPase;
DE            EC=3.1.3.11;
DE   AltName: Full=D-fructose-1,6-bisphosphate 1-phosphohydrolase;
GN   Name=CFBP;
OS   Saccharum hybrid (Sugarcane).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae;
OC   PACCAD clade; Panicoideae; Andropogoneae; Saccharum.
OX   NCBI_TaxID=15819;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. H65-7052;
RA   Grof C.P.L., Glassop D., Bugos R.C., Moore P.H.;
RT   "Sequence of a cDNA encoding cytosolic fructose-1,6-bisphosphatase
RT   from Sugarcane (Saccharum L.).";
RL   (er) Plant Gene Register PGR95-057.
CC   -!- CATALYTIC ACTIVITY: D-fructose 1,6-bisphosphate + H(2)O = D-
CC       fructose 6-phosphate + phosphate.
CC   -!- COFACTOR: Binds 3 magnesium ions per subunit (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: In plants there are two FBPase isozymes: one in the
CC       cytosol and the other in the chloroplast.
CC   -!- SIMILARITY: Belongs to the FBPase class 1 family.
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DR   EMBL; X89006; CAA61409.1; ALT_INIT; mRNA.
DR   PIR; S57717; S57717.
DR   HSSP; P00637; 1BK4.
DR   Gramene; Q43139; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042132; F:fructose 1,6-bisphosphate 1-phosphatase act...; IEA:EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR000146; Fructose_bisphosphatase.
DR   InterPro; IPR017955; IMPase/FBPase.
DR   PANTHER; PTHR11556; In_FB_phphtase; 1.
DR   Pfam; PF00316; FBPase; 1.
DR   PRINTS; PR00115; FBPHPHTASE.
DR   PRINTS; PR00377; INFBPHPHTASE.
DR   ProDom; PD001491; In_FB_phphtase; 1.
DR   PROSITE; PS00124; FBPASE; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cytoplasm; Hydrolase; Magnesium;
KW   Metal-binding.
FT   CHAIN         1    343       Fructose-1,6-bisphosphatase, cytosolic.
FT                                /FTId=PRO_0000200519.
FT   REGION      124    127       Substrate binding (By similarity).
FT   METAL        71     71       Magnesium 1 (By similarity).
FT   METAL       100    100       Magnesium 1 (By similarity).
FT   METAL       100    100       Magnesium 2 (By similarity).
FT   METAL       121    121       Magnesium 2 (By similarity).
FT   METAL       121    121       Magnesium 3 (By similarity).
FT   METAL       123    123       Magnesium 2; via carbonyl oxygen (By
FT                                similarity).
FT   METAL       124    124       Magnesium 3 (By similarity).
FT   METAL       283    283       Magnesium 3 (By similarity).
FT   BINDING     215    215       Substrate (By similarity).
FT   BINDING     247    247       Substrate (By similarity).
FT   BINDING     267    267       Substrate (By similarity).
FT   BINDING     277    277       Substrate (By similarity).
SQ   SEQUENCE   343 AA;  37409 MW;  9C15B43B6935CDE9 CRC64;
     MDHAADAHRT DLMTITRHVL NEQSRNPESR GDFTILLSHI VLGCKFVASA VNKAGLAQLI
     GLAGETNVQG EEQKKLDVLS NEVFVKALVS SGRTCVLVSE EDEETTFVDP KLRGKYCVCF
     DPLDGSSNID CGVSIGTIFG IYMIKDKDNV TLSDVLQPGK DMLAAGYCMY GSSCTLVLST
     GTGVNGFTLD PSLGEFILTH PDIKIPKKGK IYSVNEGNAK NWDVPVAKFV EKCKYPKDGS
     PPKSLRYIGS MVADVHRTLL YGGVFLYPAD QKSPNGKLRV LYEVFPMSFL MEQAGGQSFT
     GKERALDLVP TKIHERSPIF LGSYDDVEEI KALYAEQAKS SSA
//