Q43135 (C79A1_SORBI) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 83.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Tyrosine N-monooxygenase EC=1.14.13.41 Alternative name(s): Cytochrome P450 79A1 Cytochrome P450Tyr | ||||
| Gene names |
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| Organism | Sorghum bicolor (Sorghum) (Sorghum vulgare) | ||||
| Taxonomic identifier | 4558 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Sorghum |
Protein attributes
| Sequence length | 558 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | N-hydroxylase that converts L-tyrosine to p-hydroxyphenylacetaldehyde oxime. |
| Catalytic activity | L-tyrosine + O2 + NADPH = N-hydroxy-L-tyrosine + NADP+ + H2O. N-hydroxy-L-tyrosine + O2 + NADPH = N,N-dihydroxy-L-tyrosine + NADP+ + H2O. N,N-dihydroxy-L-tyrosine = (Z)-[4-hydroxyphenylacetaldehyde oxime] + CO2 + H2O. |
| Cofactor | Heme group By similarity. |
| Pathway | Secondary metabolite biosynthesis; dhurrin biosynthesis; dhurrin from L-tyrosine: step 1/3. |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass membrane protein Potential. |
| Sequence similarities | Belongs to the cytochrome P450 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane Transmembrane helix |
| Ligand | Heme Iron Metal-binding NADP |
| Molecular function | Monooxygenase Oxidoreductase |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | electron carrier activity Inferred from electronic annotation. Source: InterPro heme bindingInferred from electronic annotation. Source: InterPro tyrosine N-monooxygenase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| [1] | "The primary sequence of cytochrome P450tyr, the multifunctional N-hydroxylase catalyzing the conversion of L-tyrosine to p-hydroxyphenylacetaldehyde oxime in the biosynthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor (L.) Moench." Koch B.M., Sibbesen O., Halkier B.A., Svendsen I., Moeller B.L. Arch. Biochem. Biophys. 323:177-186(1995) [PubMed: 7487064] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 197-204; 277-282; 330-361; 363-376; 390-399; 454-460; 473-480; 484-490 AND 532-558. Strain: cv. SS1000. Tissue: Etiolated seedling. |
| [2] | "Isolation of the heme-thiolate enzyme cytochrome P-450TYR, which catalyzes the committed step in the biosynthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor (L.) Moench." Sibbesen O., Koch B., Halkier B.A., Moller B.L. Proc. Natl. Acad. Sci. U.S.A. 91:9740-9744(1994) [PubMed: 7937883] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-17. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U32624 mRNA. Translation: AAA85440.1. |
| PIR | S68203. |
| RefSeq | XP_002466099.1. XM_002466054.1. |
| UniGene | Sbi.13772. |
3D structure databases | |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | Sb01g001200.1; Sb01g001200.1; Sb01g001200. |
| GeneID | 8061413. |
| KEGG | sbi:SORBI_01g001200. |
Organism-specific databases | |
| Gramene | Q43135. |
Phylogenomic databases | |
| GeneTree | EPGT00070000027894. |
| OMA | WKLAGSE. |
| PhylomeDB | Q43135. |
| ProtClustDB | CLSN2723637. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MONOMER-521. |
Family and domain databases | |
| InterPro | IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view] |
| Gene3D | G3DSA:1.10.630.10. Cyt_P450. 1 hit. |
| KO | K13027. |
| Pfam | PF00067. p450. 1 hit. [Graphical view] |
| PRINTS | PR00463. EP450I. PR00385. P450. |
| SUPFAM | SSF48264. Cytochrome_P450. 1 hit. |
| PROSITE | PS00086. CYTOCHROME_P450. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | C79A1_SORBI | ||||||||
| Accession | Primary (citable) accession number: Q43135 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with