Reviewed, UniProtKB/Swiss-Prot Q43133 (GGPPS_SINAL)

Names and origin

Protein names

Recommended name:
    Geranylgeranyl pyrophosphate synthetase, chloroplastic/chromoplastic
      Short name=GGPP synthetase
      Short name=GGPS
Including the following 3 domains:
    1- Recommended name:
            Dimethylallyltranstransferase
              EC=2.5.1.1
    2- Recommended name:
            Geranyltranstransferase
              EC=2.5.1.10
    3- Recommended name:
            Farnesyltranstransferase
              EC=2.5.1.29

Gene names

Name:	GGPS1
Synonyms:	GGPS

Organism

Sinapis alba (White mustard) (Brassica hirta)

Taxonomic identifier

3728 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Sinapis

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate.
Catalytic activity	Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate. Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate. Trans,trans-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.
Pathway	Isoprenoid biosynthesis; farnesyl-PP biosynthesis; farnesyl-PP from geranyl-PP and isopentenyl-PP: step 1/1. Isoprenoid biosynthesis; geranyl-PP biosynthesis; geranyl-PP from dimethylallyl-PP and isopentenyl-PP: step 1/1. Isoprenoid biosynthesis; geranylgeranyl-PP biosynthesis; geranylgeranyl-PP from farnesyl-PP and isopentenyl-PP: step 1/1.
Subunit structure	Monomer.
Subcellular location	Plastid › chloroplast stromaProbable. Plastid › chromoplastProbable.
Sequence similarities	Belongs to the FPP/GGPP synthetase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis Isoprene biosynthesis
Cellular component	Chloroplast Chromoplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Technical term	3D-structure Multifunctional enzyme
Gene Ontology (GO)
Biological process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW chromoplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dimethylallyltranstransferase activity Inferred from electronic annotation. Source: EC farnesyltranstransferase activity Inferred from electronic annotation. Source: EC geranyltranstransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Chloroplast and chromoplast

Chain

? – 366

Geranylgeranyl pyrophosphate synthetase, chloroplastic/chromoplastic

PRO_0000016474

Secondary structure

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366

Helix Strand Turn

Details...

Helix

74 – 90

17

Helix

97 – 108

12

Helix

114 – 125

12

Helix

130 – 151

22

Helix

174 – 195

22

Helix

202 – 216

15

Turn

217 – 219

3

Helix

221 – 229

9

Helix

240 – 251

12

Helix

253 – 266

14

Helix

271 – 297

27

Turn

314 – 316

3

Helix

319 – 322

4

Helix

325 – 342

18

Turn

343 – 345

3

Helix

348 – 362

15

Sequences

	Sequence		Length	Mass (Da)
	Q43133-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1EADBFD9C4CF4F77	FASTA	366	39,432
	10 20 30 40 50 60 MASSVTPLGS WVLLHHHPST ILTQSRSRSP PSLITLKPIS LTPKRTVSSS SSSSLITKED 70 80 90 100 110 120 NNLKSSSSSF DFMSYIIRKA DSVNKALDSA VPLREPLKIH EAMRYSLLAG GKRVRPVLCI 130 140 150 160 170 180 AACELVGGEE SLAMPARCAV EMIHTMSLIH DDLPCMDNDD LRRGKPTNHK VYGEDVAVLA 190 200 210 220 230 240 GDALLSFAFE HLASATSSEV SPARVVRAVG ELAKAIGTEG LVAGQVVDIS SEGLDLNNVG 250 260 270 280 290 300 LEHLKFIHLH KTAALLEASA VLGGIIGGGS DEEIERLRKF ARCIGLLFQV VDDILDVTKS 310 320 330 340 350 360 SQELGKTAGK DLIADKLTYP KLMGLEKSRE FAEKLNTEAR DQLLGFDSDK VAPLLALANY IANRQN

References

[1]	"Chloroplast import of four carotenoid biosynthetic enzymes in vitro reveals differential fates prior to membrane binding and oligomeric assembly." Bonk M., Hoffmann B., von Lintig J., Schledz M., Al-Babili S., Hobeika E., Kleinig H., Beyer P. Eur. J. Biochem. 247:942-950(1997) [PubMed: 9288918] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X98795 mRNA. Translation: CAA67330.1.

PIR

T10452.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2J1O	X-ray	2.00	A	74-366	[»]
2J1P	X-ray	1.80	A/B	71-366	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
IPR008949. Terpenoid_synth.
[Graphical view]

Gene3D

G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.

PANTHER

PTHR12001. Polyprenyl_synt. 1 hit.

Pfam

PF00348. polyprenyl_synt. 1 hit.
[Graphical view]

PROSITE

PS00723. POLYPRENYL_SYNTHET_1. 1 hit.
PS00444. POLYPRENYL_SYNTHET_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GGPPS_SINAL

Accession

Primary (citable) accession number: Q43133

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1996
Last modified:	November 4, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)