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Protein

Geranylgeranyl pyrophosphate synthase, chloroplastic/chromoplastic

Gene

GGPS1

Organism
Sinapis alba (White mustard) (Brassica hirta)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate.

Catalytic activityi

Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate.
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Pathwayi: farnesyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Geranylgeranyl pyrophosphate synthase, chloroplastic/chromoplastic (GGPS1)
This subpathway is part of the pathway farnesyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate, the pathway farnesyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: geranyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Geranylgeranyl pyrophosphate synthase, chloroplastic/chromoplastic (GGPS1)
This subpathway is part of the pathway geranyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate, the pathway geranyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: geranylgeranyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Geranylgeranyl pyrophosphate synthase, chloroplastic/chromoplastic (GGPS1)
This subpathway is part of the pathway geranylgeranyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate, the pathway geranylgeranyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei112 – 1121Isopentenyl diphosphate
Binding sitei115 – 1151Isopentenyl diphosphate
Binding sitei144 – 1441Isopentenyl diphosphate
Metal bindingi151 – 1511Magnesium 1By similarity
Metal bindingi151 – 1511Magnesium 2By similarity
Metal bindingi157 – 1571Magnesium 1By similarity
Metal bindingi157 – 1571Magnesium 2By similarity
Binding sitei162 – 1621Dimethylallyl diphosphateBy similarity
Binding sitei163 – 1631Isopentenyl diphosphate
Binding sitei251 – 2511Dimethylallyl diphosphateBy similarity
Binding sitei252 – 2521Dimethylallyl diphosphateBy similarity
Binding sitei289 – 2891Dimethylallyl diphosphateBy similarity
Metal bindingi292 – 2921Magnesium 3By similarity
Binding sitei306 – 3061Dimethylallyl diphosphateBy similarity
Binding sitei316 – 3161Dimethylallyl diphosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carotenoid biosynthesis, Isoprene biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi2.5.1.29. 5734.
UniPathwayiUPA00259; UER00368.
UPA00260; UER00369.
UPA00389; UER00564.

Names & Taxonomyi

Protein namesi
Recommended name:
Geranylgeranyl pyrophosphate synthase, chloroplastic/chromoplastic (EC:2.5.1.-)
Short name:
GGPP synthase
Short name:
GGPS
Alternative name(s):
(2E,6E)-farnesyl diphosphate synthase
Dimethylallyltranstransferase (EC:2.5.1.1)
Farnesyl diphosphate synthase
Farnesyltranstransferase (EC:2.5.1.29)
Geranyltranstransferase (EC:2.5.1.10)
Gene namesi
Name:GGPS1
Synonyms:GGPS
OrganismiSinapis alba (White mustard) (Brassica hirta)
Taxonomic identifieri3728 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeSinapis

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Chromoplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 366Geranylgeranyl pyrophosphate synthase, chloroplastic/chromoplasticPRO_0000016474
Transit peptidei1 – ?Chloroplast and chromoplast

Interactioni

Subunit structurei

Dimer.

Structurei

Secondary structure

1
366
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi74 – 9017Combined sources
Helixi97 – 10711Combined sources
Helixi114 – 12512Combined sources
Helixi130 – 15122Combined sources
Turni154 – 1574Combined sources
Beta strandi160 – 1623Combined sources
Helixi168 – 1725Combined sources
Helixi174 – 19522Combined sources
Helixi202 – 21615Combined sources
Turni218 – 2203Combined sources
Helixi221 – 2277Combined sources
Helixi236 – 25116Combined sources
Helixi253 – 26614Combined sources
Helixi271 – 29828Combined sources
Turni314 – 3163Combined sources
Helixi319 – 34224Combined sources
Turni343 – 3453Combined sources
Turni348 – 3514Combined sources
Helixi352 – 36312Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2J1OX-ray2.00A74-366[»]
2J1PX-ray1.80A/B74-366[»]
ProteinModelPortaliQ43133.
SMRiQ43133. Positions 71-361.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ43133.

Family & Domainsi

Sequence similaritiesi

Belongs to the FPP/GGPP synthase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
[Graphical view]
PANTHERiPTHR12001. PTHR12001. 1 hit.
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00723. POLYPRENYL_SYNTHASE_1. 1 hit.
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43133-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSVTPLGS WVLLHHHPST ILTQSRSRSP PSLITLKPIS LTPKRTVSSS
60 70 80 90 100
SSSSLITKED NNLKSSSSSF DFMSYIIRKA DSVNKALDSA VPLREPLKIH
110 120 130 140 150
EAMRYSLLAG GKRVRPVLCI AACELVGGEE SLAMPARCAV EMIHTMSLIH
160 170 180 190 200
DDLPCMDNDD LRRGKPTNHK VYGEDVAVLA GDALLSFAFE HLASATSSEV
210 220 230 240 250
SPARVVRAVG ELAKAIGTEG LVAGQVVDIS SEGLDLNNVG LEHLKFIHLH
260 270 280 290 300
KTAALLEASA VLGGIIGGGS DEEIERLRKF ARCIGLLFQV VDDILDVTKS
310 320 330 340 350
SQELGKTAGK DLIADKLTYP KLMGLEKSRE FAEKLNTEAR DQLLGFDSDK
360
VAPLLALANY IANRQN
Length:366
Mass (Da):39,432
Last modified:November 1, 1996 - v1
Checksum:i1EADBFD9C4CF4F77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98795 mRNA. Translation: CAA67330.1.
PIRiT10452.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98795 mRNA. Translation: CAA67330.1.
PIRiT10452.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2J1OX-ray2.00A74-366[»]
2J1PX-ray1.80A/B74-366[»]
ProteinModelPortaliQ43133.
SMRiQ43133. Positions 71-361.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00259; UER00368.
UPA00260; UER00369.
UPA00389; UER00564.
BRENDAi2.5.1.29. 5734.

Miscellaneous databases

EvolutionaryTraceiQ43133.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
[Graphical view]
PANTHERiPTHR12001. PTHR12001. 1 hit.
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00723. POLYPRENYL_SYNTHASE_1. 1 hit.
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Chloroplast import of four carotenoid biosynthetic enzymes in vitro reveals differential fates prior to membrane binding and oligomeric assembly."
    Bonk M., Hoffmann B., von Lintig J., Schledz M., Al-Babili S., Hobeika E., Kleinig H., Beyer P.
    Eur. J. Biochem. 247:942-950(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGGPPS_SINAL
AccessioniPrimary (citable) accession number: Q43133
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1996
Last modified: October 14, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.