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Protein

Geranylgeranyl pyrophosphate synthase, chloroplastic/chromoplastic

Gene

GGPS1

Organism
Sinapis alba (White mustard) (Brassica hirta)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate.

Catalytic activityi

Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate.
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Pathwayi: farnesyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Geranylgeranyl pyrophosphate synthase, chloroplastic/chromoplastic (GGPS1)
This subpathway is part of the pathway farnesyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate, the pathway farnesyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: geranyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Geranylgeranyl pyrophosphate synthase, chloroplastic/chromoplastic (GGPS1)
This subpathway is part of the pathway geranyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate, the pathway geranyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: geranylgeranyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Geranylgeranyl pyrophosphate synthase, chloroplastic/chromoplastic (GGPS1)
This subpathway is part of the pathway geranylgeranyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate, the pathway geranylgeranyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112Isopentenyl diphosphate1
Binding sitei115Isopentenyl diphosphate1
Binding sitei144Isopentenyl diphosphate1
Metal bindingi151Magnesium 1By similarity1
Metal bindingi151Magnesium 2By similarity1
Metal bindingi157Magnesium 1By similarity1
Metal bindingi157Magnesium 2By similarity1
Binding sitei162Dimethylallyl diphosphateBy similarity1
Binding sitei163Isopentenyl diphosphate1
Binding sitei251Dimethylallyl diphosphateBy similarity1
Binding sitei252Dimethylallyl diphosphateBy similarity1
Binding sitei289Dimethylallyl diphosphateBy similarity1
Metal bindingi292Magnesium 3By similarity1
Binding sitei306Dimethylallyl diphosphateBy similarity1
Binding sitei316Dimethylallyl diphosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carotenoid biosynthesis, Isoprene biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi2.5.1.29. 5734.
UniPathwayiUPA00259; UER00368.
UPA00260; UER00369.
UPA00389; UER00564.

Names & Taxonomyi

Protein namesi
Recommended name:
Geranylgeranyl pyrophosphate synthase, chloroplastic/chromoplastic (EC:2.5.1.-)
Short name:
GGPP synthase
Short name:
GGPS
Alternative name(s):
(2E,6E)-farnesyl diphosphate synthase
Dimethylallyltranstransferase (EC:2.5.1.1)
Farnesyl diphosphate synthase
Farnesyltranstransferase (EC:2.5.1.29)
Geranyltranstransferase (EC:2.5.1.10)
Gene namesi
Name:GGPS1
Synonyms:GGPS
OrganismiSinapis alba (White mustard) (Brassica hirta)
Taxonomic identifieri3728 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeSinapis

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Chromoplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000016474? – 366Geranylgeranyl pyrophosphate synthase, chloroplastic/chromoplastic
Transit peptidei1 – ?Chloroplast and chromoplast

Interactioni

Subunit structurei

Dimer.

Structurei

Secondary structure

1366
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi74 – 90Combined sources17
Helixi97 – 107Combined sources11
Helixi114 – 125Combined sources12
Helixi130 – 151Combined sources22
Turni154 – 157Combined sources4
Beta strandi160 – 162Combined sources3
Helixi168 – 172Combined sources5
Helixi174 – 195Combined sources22
Helixi202 – 216Combined sources15
Turni218 – 220Combined sources3
Helixi221 – 227Combined sources7
Helixi236 – 251Combined sources16
Helixi253 – 266Combined sources14
Helixi271 – 298Combined sources28
Turni314 – 316Combined sources3
Helixi319 – 342Combined sources24
Turni343 – 345Combined sources3
Turni348 – 351Combined sources4
Helixi352 – 363Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J1OX-ray2.00A74-366[»]
2J1PX-ray1.80A/B74-366[»]
ProteinModelPortaliQ43133.
SMRiQ43133.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ43133.

Family & Domainsi

Sequence similaritiesi

Belongs to the FPP/GGPP synthase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
IPR033749. Polyprenyl_synt_CS.
[Graphical view]
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00723. POLYPRENYL_SYNTHASE_1. 1 hit.
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43133-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSVTPLGS WVLLHHHPST ILTQSRSRSP PSLITLKPIS LTPKRTVSSS
60 70 80 90 100
SSSSLITKED NNLKSSSSSF DFMSYIIRKA DSVNKALDSA VPLREPLKIH
110 120 130 140 150
EAMRYSLLAG GKRVRPVLCI AACELVGGEE SLAMPARCAV EMIHTMSLIH
160 170 180 190 200
DDLPCMDNDD LRRGKPTNHK VYGEDVAVLA GDALLSFAFE HLASATSSEV
210 220 230 240 250
SPARVVRAVG ELAKAIGTEG LVAGQVVDIS SEGLDLNNVG LEHLKFIHLH
260 270 280 290 300
KTAALLEASA VLGGIIGGGS DEEIERLRKF ARCIGLLFQV VDDILDVTKS
310 320 330 340 350
SQELGKTAGK DLIADKLTYP KLMGLEKSRE FAEKLNTEAR DQLLGFDSDK
360
VAPLLALANY IANRQN
Length:366
Mass (Da):39,432
Last modified:November 1, 1996 - v1
Checksum:i1EADBFD9C4CF4F77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98795 mRNA. Translation: CAA67330.1.
PIRiT10452.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98795 mRNA. Translation: CAA67330.1.
PIRiT10452.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J1OX-ray2.00A74-366[»]
2J1PX-ray1.80A/B74-366[»]
ProteinModelPortaliQ43133.
SMRiQ43133.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00259; UER00368.
UPA00260; UER00369.
UPA00389; UER00564.
BRENDAi2.5.1.29. 5734.

Miscellaneous databases

EvolutionaryTraceiQ43133.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR000092. Polyprenyl_synt.
IPR033749. Polyprenyl_synt_CS.
[Graphical view]
PfamiPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS00723. POLYPRENYL_SYNTHASE_1. 1 hit.
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGGPPS_SINAL
AccessioniPrimary (citable) accession number: Q43133
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.