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Protein

Enolase

Gene

PGH1

Organism
Mesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase, cytosolic (GAPC)
  2. no protein annotated in this organism
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (PGM1)
  4. Enolase (PGH1)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei163SubstrateBy similarity1
Binding sitei172SubstrateBy similarity1
Active sitei215Proton donorBy similarity1
Metal bindingi250MagnesiumBy similarity1
Metal bindingi300MagnesiumBy similarity1
Binding sitei300SubstrateBy similarity1
Metal bindingi327MagnesiumBy similarity1
Binding sitei327SubstrateBy similarity1
Active sitei352Proton acceptorBy similarity1
Binding sitei403SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:PGH1
Synonyms:PGH1B
OrganismiMesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum)
Taxonomic identifieri3544 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAizoaceaeMesembryanthemumCryophytum

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340751 – 444EnolaseAdd BLAST444

Proteomic databases

PRIDEiQ43130.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ43130.
SMRiQ43130.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni379 – 382Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q43130-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTIKCVKAR QIYDSRGNPT VEADIHLDDG TYARAAVPSG ASTGVYEALE
60 70 80 90 100
LRDGGKDYMG KGVYKAVKNV NEIIGPALVG KDPTQQTAID NFMVQQLDGT
110 120 130 140 150
VNEWGWCKQK LGANAILAVS LAVCKAGAQV KKIPLYQHIA EIAGNKNMVL
160 170 180 190 200
PVPAFNVING GSHAGNKLAM QEFMILPTGA SSFKEAMKMG SEVYHNLKSV
210 220 230 240 250
IKKKYGQDAT NVGDEGGFAP NIQENKEGLE LLKTAIEKAG YTGKVVIGMD
260 270 280 290 300
VAASEFYKED KSYDLNFKEE NNDGSQRISG EALKDLYKSF VAEYPIVSIE
310 320 330 340 350
DPFDQDDWEH YAKMTAECGE KVQIVGDDLL VTNPKRVKKA IDENPCNALL
360 370 380 390 400
LKVNQIGSVT ESIEAVKMSK KAGWGVMASH RSGETEDTFI ADLSVGLSTG
410 420 430 440
QIKTGAPCRS ERLAKYNQLL RIEEELGDKA VYAGANFRRP VEPY
Length:444
Mass (Da):48,349
Last modified:November 1, 1996 - v1
Checksum:i044E21DF1E5BA166
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti345P → S. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79242 mRNA. Translation: AAB34986.1.
U09194 mRNA. Translation: AAA21277.1.
PIRiT12341.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENO_MESCR
AccessioniPrimary (citable) accession number: Q43130
Secondary accession number(s): Q42907
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: April 12, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families