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Q43127

- GLNA2_ARATH

UniProt

Q43127 - GLNA2_ARATH

Protein

Glutamine synthetase, chloroplastic/mitochondrial

Gene

GLN2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    The light-modulated chloroplast/mitochondrial enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.1 Publication

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: TAIR

    GO - Biological processi

    1. aging Source: TAIR
    2. ammonia assimilation cycle Source: TAIR
    3. glutamine biosynthetic process Source: InterPro
    4. response to cadmium ion Source: TAIR
    5. response to salt stress Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G35630-MONOMER.
    ARA:GQT-2612-MONOMER.
    ARA:GQT-2613-MONOMER.
    MetaCyc:AT5G35630-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase, chloroplastic/mitochondrial (EC:6.3.1.2)
    Alternative name(s):
    GS2
    Glutamate--ammonia ligase
    Gene namesi
    Name:GLN2
    Synonyms:GSLI
    Ordered Locus Names:At5g35630
    ORF Names:MJE4.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G35630.

    Subcellular locationi

    Plastidchloroplast 1 Publication. Mitochondrion 1 Publication

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. chloroplast Source: TAIR
    3. chloroplast envelope Source: TAIR
    4. chloroplast stroma Source: TAIR
    5. chloroplast thylakoid membrane Source: TAIR
    6. cytosolic ribosome Source: TAIR
    7. membrane Source: TAIR
    8. mitochondrion Source: TAIR
    9. thylakoid Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Mitochondrion, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4545Chloroplast and mitochondrionSequence AnalysisAdd
    BLAST
    Chaini46 – 430385Glutamine synthetase, chloroplastic/mitochondrialPRO_0000011174Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei106 – 1061Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ43127.
    PRIDEiQ43127.

    2D gel databases

    SWISS-2DPAGEQ43127.

    Expressioni

    Tissue specificityi

    Expressed in mesophyll and epidermal cells of leaves.1 Publication

    Inductioni

    By light, sucrose, glucose and fructose.1 Publication

    Gene expression databases

    ArrayExpressiQ43127.
    GenevestigatoriQ43127.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    BioGridi18788. 6 interactions.
    DIPiDIP-32735N.
    IntActiQ43127. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ43127.
    SMRiQ43127. Positions 65-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000061500.
    InParanoidiQ43127.
    KOiK01915.
    OMAiIIVETTC.
    PhylomeDBiQ43127.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q43127-1 [UniParc]FASTAAdd to Basket

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    MAQILAASPT CQMRVPKHSS VIASSSKLWS SVVLKQKKQS NNKVRGFRVL    50
    ALQSDNSTVN RVETLLNLDT KPYSDRIIAE YIWIGGSGID LRSKSRTIEK 100
    PVEDPSELPK WNYDGSSTGQ APGEDSEVIL YPQAIFRDPF RGGNNILVIC 150
    DTWTPAGEPI PTNKRAKAAE IFSNKKVSGE VPWFGIEQEY TLLQQNVKWP 200
    LGWPVGAFPG PQGPYYCGVG ADKIWGRDIS DAHYKACLYA GINISGTNGE 250
    VMPGQWEFQV GPSVGIDAGD HVWCARYLLE RITEQAGVVL TLDPKPIEGD 300
    WNGAGCHTNY STKSMREEGG FEVIKKAILN LSLRHKEHIS AYGEGNERRL 350
    TGKHETASID QFSWGVANRG CSIRVGRDTE AKGKGYLEDR RPASNMDPYI 400
    VTSLLAETTL LWEPTLEAEA LAAQKLSLNV 430
    Length:430
    Mass (Da):47,411
    Last modified:November 1, 1996 - v1
    Checksum:i664029BC06572295
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S69727 mRNA. Translation: AAB20558.1.
    AB015045 Genomic DNA. Translation: BAA88761.1.
    AB013393 Genomic DNA. Translation: BAB09304.1.
    CP002688 Genomic DNA. Translation: AED93993.1.
    CP002688 Genomic DNA. Translation: AED93994.1.
    CP002688 Genomic DNA. Translation: AED93995.1.
    AF428319 mRNA. Translation: AAL16249.1.
    AF428461 mRNA. Translation: AAL16230.1.
    AY081252 mRNA. Translation: AAL91141.1.
    AY091114 mRNA. Translation: AAM14064.1.
    AY122977 mRNA. Translation: AAM67510.1.
    AY088222 mRNA. Translation: AAM65763.1.
    PIRiS18600.
    RefSeqiNP_001031969.1. NM_001036892.1.
    NP_001078639.1. NM_001085170.1.
    NP_198413.1. NM_122954.3.
    UniGeneiAt.24218.

    Genome annotation databases

    EnsemblPlantsiAT5G35630.1; AT5G35630.1; AT5G35630.
    AT5G35630.2; AT5G35630.2; AT5G35630.
    AT5G35630.3; AT5G35630.3; AT5G35630.
    GeneIDi833535.
    KEGGiath:AT5G35630.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S69727 mRNA. Translation: AAB20558.1 .
    AB015045 Genomic DNA. Translation: BAA88761.1 .
    AB013393 Genomic DNA. Translation: BAB09304.1 .
    CP002688 Genomic DNA. Translation: AED93993.1 .
    CP002688 Genomic DNA. Translation: AED93994.1 .
    CP002688 Genomic DNA. Translation: AED93995.1 .
    AF428319 mRNA. Translation: AAL16249.1 .
    AF428461 mRNA. Translation: AAL16230.1 .
    AY081252 mRNA. Translation: AAL91141.1 .
    AY091114 mRNA. Translation: AAM14064.1 .
    AY122977 mRNA. Translation: AAM67510.1 .
    AY088222 mRNA. Translation: AAM65763.1 .
    PIRi S18600.
    RefSeqi NP_001031969.1. NM_001036892.1.
    NP_001078639.1. NM_001085170.1.
    NP_198413.1. NM_122954.3.
    UniGenei At.24218.

    3D structure databases

    ProteinModelPortali Q43127.
    SMRi Q43127. Positions 65-413.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 18788. 6 interactions.
    DIPi DIP-32735N.
    IntActi Q43127. 2 interactions.

    2D gel databases

    SWISS-2DPAGE Q43127.

    Proteomic databases

    PaxDbi Q43127.
    PRIDEi Q43127.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G35630.1 ; AT5G35630.1 ; AT5G35630 .
    AT5G35630.2 ; AT5G35630.2 ; AT5G35630 .
    AT5G35630.3 ; AT5G35630.3 ; AT5G35630 .
    GeneIDi 833535.
    KEGGi ath:AT5G35630.

    Organism-specific databases

    TAIRi AT5G35630.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000061500.
    InParanoidi Q43127.
    KOi K01915.
    OMAi IIVETTC.
    PhylomeDBi Q43127.

    Enzyme and pathway databases

    BioCyci ARA:AT5G35630-MONOMER.
    ARA:GQT-2612-MONOMER.
    ARA:GQT-2613-MONOMER.
    MetaCyc:AT5G35630-MONOMER.

    Gene expression databases

    ArrayExpressi Q43127.
    Genevestigatori Q43127.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The glutamine synthetase gene family of Arabidopsis thaliana: light-regulation and differential expression in leaves, roots and seeds."
      Peterman T.K., Goodman H.M.
      Mol. Gen. Genet. 230:145-154(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Nucleotide sequences of genes for cytosolic and chloroplastic glutamine synthetase from Arabidopsis thaliana."
      Arimura G., Fujii M., Takahashi M., Goshima N., Morikawa H.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
      Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
      DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. "Carbon and amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis."
      Oliveira I.C., Coruzzi G.M.
      Plant Physiol. 121:301-310(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    8. "Arabidopsis thaliana GLN2-encoded glutamine synthetase is dual targeted to leaf mitochondria and chloroplasts."
      Taira M., Valtersson U., Burkhardt B., Ludwig R.A.
      Plant Cell 16:2048-2058(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    9. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGLNA2_ARATH
    AccessioniPrimary (citable) accession number: Q43127
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3