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Q43127

- GLNA2_ARATH

UniProt

Q43127 - GLNA2_ARATH

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Protein
Glutamine synthetase, chloroplastic/mitochondrial
Gene
GLN2, GSLI, At5g35630, MJE4.9
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The light-modulated chloroplast/mitochondrial enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.1 Publication

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: TAIR

GO - Biological processi

  1. aging Source: TAIR
  2. ammonia assimilation cycle Source: TAIR
  3. glutamine biosynthetic process Source: InterPro
  4. response to cadmium ion Source: TAIR
  5. response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G35630-MONOMER.
ARA:GQT-2612-MONOMER.
ARA:GQT-2613-MONOMER.
MetaCyc:AT5G35630-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase, chloroplastic/mitochondrial (EC:6.3.1.2)
Alternative name(s):
GS2
Glutamate--ammonia ligase
Gene namesi
Name:GLN2
Synonyms:GSLI
Ordered Locus Names:At5g35630
ORF Names:MJE4.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G35630.

Subcellular locationi

Plastidchloroplast. Mitochondrion 1 Publication

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. chloroplast Source: TAIR
  3. chloroplast envelope Source: TAIR
  4. chloroplast stroma Source: TAIR
  5. chloroplast thylakoid membrane Source: TAIR
  6. cytosolic ribosome Source: TAIR
  7. membrane Source: TAIR
  8. mitochondrion Source: TAIR
  9. thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4545Chloroplast and mitochondrion Reviewed prediction
Add
BLAST
Chaini46 – 430385Glutamine synthetase, chloroplastic/mitochondrial
PRO_0000011174Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei106 – 1061Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ43127.
PRIDEiQ43127.

2D gel databases

SWISS-2DPAGEQ43127.

Expressioni

Tissue specificityi

Expressed in mesophyll and epidermal cells of leaves.1 Publication

Inductioni

By light, sucrose, glucose and fructose.1 Publication

Gene expression databases

ArrayExpressiQ43127.
GenevestigatoriQ43127.

Interactioni

Subunit structurei

Homooctamer By similarity.

Protein-protein interaction databases

BioGridi18788. 6 interactions.
DIPiDIP-32735N.
IntActiQ43127. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ43127.
SMRiQ43127. Positions 65-413.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiQ43127.
KOiK01915.
OMAiIIVETTC.
PhylomeDBiQ43127.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43127-1 [UniParc]FASTAAdd to Basket

« Hide

MAQILAASPT CQMRVPKHSS VIASSSKLWS SVVLKQKKQS NNKVRGFRVL    50
ALQSDNSTVN RVETLLNLDT KPYSDRIIAE YIWIGGSGID LRSKSRTIEK 100
PVEDPSELPK WNYDGSSTGQ APGEDSEVIL YPQAIFRDPF RGGNNILVIC 150
DTWTPAGEPI PTNKRAKAAE IFSNKKVSGE VPWFGIEQEY TLLQQNVKWP 200
LGWPVGAFPG PQGPYYCGVG ADKIWGRDIS DAHYKACLYA GINISGTNGE 250
VMPGQWEFQV GPSVGIDAGD HVWCARYLLE RITEQAGVVL TLDPKPIEGD 300
WNGAGCHTNY STKSMREEGG FEVIKKAILN LSLRHKEHIS AYGEGNERRL 350
TGKHETASID QFSWGVANRG CSIRVGRDTE AKGKGYLEDR RPASNMDPYI 400
VTSLLAETTL LWEPTLEAEA LAAQKLSLNV 430
Length:430
Mass (Da):47,411
Last modified:November 1, 1996 - v1
Checksum:i664029BC06572295
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S69727 mRNA. Translation: AAB20558.1.
AB015045 Genomic DNA. Translation: BAA88761.1.
AB013393 Genomic DNA. Translation: BAB09304.1.
CP002688 Genomic DNA. Translation: AED93993.1.
CP002688 Genomic DNA. Translation: AED93994.1.
CP002688 Genomic DNA. Translation: AED93995.1.
AF428319 mRNA. Translation: AAL16249.1.
AF428461 mRNA. Translation: AAL16230.1.
AY081252 mRNA. Translation: AAL91141.1.
AY091114 mRNA. Translation: AAM14064.1.
AY122977 mRNA. Translation: AAM67510.1.
AY088222 mRNA. Translation: AAM65763.1.
PIRiS18600.
RefSeqiNP_001031969.1. NM_001036892.1.
NP_001078639.1. NM_001085170.1.
NP_198413.1. NM_122954.3.
UniGeneiAt.24218.

Genome annotation databases

EnsemblPlantsiAT5G35630.1; AT5G35630.1; AT5G35630.
AT5G35630.2; AT5G35630.2; AT5G35630.
AT5G35630.3; AT5G35630.3; AT5G35630.
GeneIDi833535.
KEGGiath:AT5G35630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S69727 mRNA. Translation: AAB20558.1 .
AB015045 Genomic DNA. Translation: BAA88761.1 .
AB013393 Genomic DNA. Translation: BAB09304.1 .
CP002688 Genomic DNA. Translation: AED93993.1 .
CP002688 Genomic DNA. Translation: AED93994.1 .
CP002688 Genomic DNA. Translation: AED93995.1 .
AF428319 mRNA. Translation: AAL16249.1 .
AF428461 mRNA. Translation: AAL16230.1 .
AY081252 mRNA. Translation: AAL91141.1 .
AY091114 mRNA. Translation: AAM14064.1 .
AY122977 mRNA. Translation: AAM67510.1 .
AY088222 mRNA. Translation: AAM65763.1 .
PIRi S18600.
RefSeqi NP_001031969.1. NM_001036892.1.
NP_001078639.1. NM_001085170.1.
NP_198413.1. NM_122954.3.
UniGenei At.24218.

3D structure databases

ProteinModelPortali Q43127.
SMRi Q43127. Positions 65-413.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 18788. 6 interactions.
DIPi DIP-32735N.
IntActi Q43127. 2 interactions.

2D gel databases

SWISS-2DPAGE Q43127.

Proteomic databases

PaxDbi Q43127.
PRIDEi Q43127.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G35630.1 ; AT5G35630.1 ; AT5G35630 .
AT5G35630.2 ; AT5G35630.2 ; AT5G35630 .
AT5G35630.3 ; AT5G35630.3 ; AT5G35630 .
GeneIDi 833535.
KEGGi ath:AT5G35630.

Organism-specific databases

TAIRi AT5G35630.

Phylogenomic databases

eggNOGi COG0174.
HOGENOMi HOG000061500.
InParanoidi Q43127.
KOi K01915.
OMAi IIVETTC.
PhylomeDBi Q43127.

Enzyme and pathway databases

BioCyci ARA:AT5G35630-MONOMER.
ARA:GQT-2612-MONOMER.
ARA:GQT-2613-MONOMER.
MetaCyc:AT5G35630-MONOMER.

Gene expression databases

ArrayExpressi Q43127.
Genevestigatori Q43127.

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The glutamine synthetase gene family of Arabidopsis thaliana: light-regulation and differential expression in leaves, roots and seeds."
    Peterman T.K., Goodman H.M.
    Mol. Gen. Genet. 230:145-154(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Nucleotide sequences of genes for cytosolic and chloroplastic glutamine synthetase from Arabidopsis thaliana."
    Arimura G., Fujii M., Takahashi M., Goshima N., Morikawa H.
    Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Carbon and amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis."
    Oliveira I.C., Coruzzi G.M.
    Plant Physiol. 121:301-310(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "Arabidopsis thaliana GLN2-encoded glutamine synthetase is dual targeted to leaf mitochondria and chloroplasts."
    Taira M., Valtersson U., Burkhardt B., Ludwig R.A.
    Plant Cell 16:2048-2058(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  9. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGLNA2_ARATH
AccessioniPrimary (citable) accession number: Q43127
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi