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Protein

Cryptochrome-1

Gene

CRY1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Photoreceptor that mediates primarily blue light inhibition of hypocotyl elongation and photoperiodic control of floral initiation, and regulates other light responses, including circadian rhythms, tropic growth, stomata opening, guard cell development, root development, bacterial and viral pathogen responses, abiotic stress responses, cell cycles, programmed cell death, apical dominance, fruit and ovule development, seed dormancy, and magnetoreception. Photoexcited cryptochromes interact with signaling partner proteins to alter gene expression at both transcriptional and post-translational levels and, consequently, regulate the corresponding metabolic and developmental programs (PubMed:21841916). Blue-light absorbing flavoprotein that activates reversible flavin photoreduction via an electron transport chain comprising a tryptophan triad (W-324, W-377 and W-400), accompanied by a large conformational change upon photoexcitation, or via an alternative electron transport that involves small metabolites, including NADPH, NADH, and ATP. The half-life of the activated signaling state is about 5 minutes (PubMed:26313597, PubMed:25157750, PubMed:23398192, PubMed:21875594, PubMed:21467031). Also involved in the detection of blue/green ratio in light (shade under leaf canopies) and subsequent adaptations on plant growth and development (PubMed:20668058). In darkness, the dark reoxidation of flavin occurs and lead to inactivated state (PubMed:21467031, PubMed:23398192). Perceives low blue light (LBL) and responds by directly contacting two bHLH transcription factors, PIF4 and PIF5, at chromatin on E-box variant 5'-CA[CT]GTG-3' to promote their activity and stimulate specific gene expression to adapt global physiology (e.g. hypocotyl elongation and hyponastic growth in low blue light) (PubMed:26724867, PubMed:19558423). When activated by high-intensity blue light, catalyzes direct enzymatic conversion of molecular oxygen O2 to reactive oxygen species (ROS) and hydrogen peroxide H2O2 in vitro. ROS accumulation upon activation by blue light leads to cell death in protoplasts (PubMed:25728686). Seems essential for blue-light-triggered and singlet oxygen-mediated programmed cell death (PCD) (PubMed:17075038). Required for the induction of nuclear genes encoding photoprotective components by GATA24 and GATA28 in extreme light intensities that exceed the electron utilization capacity of the chloroplast (PubMed:22786870). Involved in shortening the circadian clock period, especially at 27 degrees Celsius, in blue light (BL) and required to maintain clock genes expression rhythm (PubMed:23511208). Mediates blue light-induced gene expression and hypocotyl elongation through the inhibition of COP1-mediated degradation of the transcription factors BIT1 and HY5 and via the activation of anion channels at the plasma membrane, probably via auxin signaling (PubMed:21511872, PubMed:21511871, PubMed:16093319, PubMed:18397371, PubMed:12324610, PubMed:8528277, PubMed:9765547, PubMed:25721730). Required for the hypocotyl hook formation in darkness (PubMed:22855128). Involved in blue light-dependent stomatal opening, CHS gene expression, transpiration, inhibition of stem growth and increase of root growth, probably by regulating abscisic acid (ABA) (PubMed:22147516, PubMed:16093319, PubMed:16703358, PubMed:7756321, PubMed:9565033). Prevents lateral roots growth by inhibiting auxin transport (PubMed:20133010). Necessary for shade avoidance syndrome (SAS), characterized by leaf hyponasty and reduced lamina/petiole ratio, when exposed to blue light attenuation (PubMed:21457375). Together with phototropins, involved in phototropism regulation by various blue light fluence; blue light attenuates phototropism in high fluence rates (100 µmol.m-2.s-1) but enhances phototropism in low fluence rates (<1.0 µmol.m-2.s-1) (PubMed:12857830). Required for blue/UV-A wavelengths-mediated inhibition of explants shoot regeneration in vitro (e.g. new shoot apical meristems regeneration from excised cotyledons) (PubMed:22681544). Modulates anthocyanin accumulation in a PHYA-dependent manner in far-red-light. Acts as a PHYA/PHYB-dependent modulator of chlorophyll accumulation in red light. Contributes to most blue light deetiolation responses (PubMed:9733523, PubMed:8528277). May act as a chemical magnetoreceptor, via magnetically sensitive kinetics and quantum yields of photo-induced flavin / tryptophan radical pairs (PubMed:22421133). The effect of near-null magnetic field on flowering is altered by changes of blue light cycle and intensity in a CRY1/CRY2-dependent manner (PubMed:26095447). Involved in the strigolactone signaling that regulates hypocotyl growth in response to blue light (PubMed:24126495). Modulates temperature-dependent growth and physiology maintenance, especially at warm ambient temperatures, via HFR1-dependent activity (PubMed:21265897).1 Publication34 Publications
Implicated in promoting R protein-mediated resistance to Pseudomonas syringae pv. tomato (Pst.) DC3000 under continuous light conditions. Promotes systemic acquired resistance (SAR) and PR gene expression triggered by P. syringae.1 Publication

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Light exposure induces a conformational change in the C-terminal domain CCT1 required for activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei235FADCombined sources1 Publication1
Metal bindingi238Magnesium 1; via carbonyl oxygenCombined sources1 Publication1
Binding sitei239ATPCombined sources1 Publication1
Metal bindingi241Magnesium 2; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi244Magnesium 2Combined sources1 Publication1
Metal bindingi246Magnesium 1Combined sources1 Publication1
Metal bindingi246Magnesium 2Combined sources1 Publication1
Binding sitei293FAD; via carbonyl oxygenCombined sources1 Publication1
Sitei324Involved in electron transfer from the protein surface to the FAD cofactor1 Publication1 Publication1
Metal bindingi358Magnesium 1; via tele nitrogenCombined sources1 Publication1
Binding sitei359FADCombined sources1 Publication1
Sitei377Involved in electron transfer from the protein surface to the FAD cofactor1 Publication1
Sitei400Involved in electron transfer from the protein surface to the FAD cofactor1 Publication1 Publication1
Binding sitei409ATPCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi247 – 251FADCombined sources1 Publication5
Nucleotide bindingi359 – 360ATPCombined sources1 Publication2
Nucleotide bindingi390 – 392FADCombined sources1 Publication3

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • blue light photoreceptor activity Source: TAIR
  • FAD binding Source: UniProtKB
  • identical protein binding Source: IntAct
  • kinase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • protein kinase activity Source: TAIR

GO - Biological processi

  • anthocyanin-containing compound metabolic process Source: UniProtKB
  • auxin transport Source: UniProtKB
  • blue light signaling pathway Source: TAIR
  • circadian regulation of calcium ion oscillation Source: TAIR
  • circadian rhythm Source: UniProtKB
  • defense response Source: UniProtKB-KW
  • detection of light stimulus Source: TAIR
  • flavin adenine dinucleotide metabolic process Source: UniProtKB
  • negative regulation of lateral root development Source: UniProtKB
  • oxidation-reduction process Source: TAIR
  • photomorphogenesis Source: TAIR
  • photoprotection Source: UniProtKB
  • phototropism Source: UniProtKB
  • plant organ development Source: UniProtKB
  • positive regulation of anion channel activity Source: UniProtKB
  • positive regulation of defense response to bacterium Source: UniProtKB
  • positive regulation of shade avoidance Source: UniProtKB
  • positive regulation of systemic acquired resistance Source: UniProtKB
  • protein autophosphorylation Source: UniProtKB
  • protein-chromophore linkage Source: UniProtKB-KW
  • regulation of circadian rhythm Source: UniProtKB
  • regulation of hydrogen peroxide metabolic process Source: UniProtKB
  • regulation of leaf morphogenesis Source: UniProtKB
  • regulation of meristem growth Source: TAIR
  • regulation of reactive oxygen species metabolic process Source: UniProtKB
  • regulation of unidimensional cell growth Source: TAIR
  • response to absence of light Source: UniProtKB
  • response to blue light Source: UniProtKB
  • response to far red light Source: UniProtKB
  • response to high light intensity Source: UniProtKB
  • response to light stimulus Source: UniProtKB
  • response to low fluence blue light stimulus by blue low-fluence system Source: UniProtKB
  • response to magnetism Source: UniProtKB
  • response to red light Source: UniProtKB
  • response to strigolactone Source: UniProtKB
  • response to water deprivation Source: TAIR
  • singlet oxygen-mediated programmed cell death Source: TAIR
  • stomatal movement Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Photoreceptor protein, Receptor

Keywords - Biological processi

Apoptosis, Plant defense, Sensory transduction

Keywords - Ligandi

ATP-binding, Chromophore, FAD, Flavoprotein, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-2162-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cryptochrome-11 Publication
Short name:
AtCry1 Publication
Short name:
Atcry11 Publication
Alternative name(s):
Blue light photoreceptor1 Publication
Protein BLUE LIGHT UNINHIBITED 11 Publication
Protein ELONGATED HYPOCOTYL 41 Publication
Protein OUT OF PHASE 2
Short name:
OOP2
Gene namesi
Name:CRY11 Publication
Synonyms:BLU11 Publication, HY41 Publication
Ordered Locus Names:At4g08920Imported
ORF Names:T3H13.14Imported, T3H13.5Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G08920.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nuclear body Source: UniProtKB
  • nucleus Source: UniProtKB
  • PML body Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Prevents the shortening of period at 27 degrees Celsius, resulting in a long period phenotype. The double mutant cry1 cry2 is impaired in blue light signaling, resulting in long-period, lower-amplitude oscillations at 12 and 17 degrees Celsius and completely abolishing rhythms at 27 degrees Celsius (PubMed:23511208). Plants show reduced root and hypocotyl elongation in an anion channels activation-dependent manner at the plasma membrane, as well a reduced anthocyanin accumulation in blue light (PubMed:8528277, PubMed:12324610, PubMed:16703358, PubMed:21511871, PubMed:21511872, PubMed:9765547). Impaired blue/UV-A wavelengths-mediated inhibition of shoot regeneration (PubMed:22681544). Impaired detection of blue/green ratio in light leading to abnormal inhibition of hypocotyl growth (PubMed:20668058). Reduced attenuating effect of high fluence rates of blue light. This phenotype is stronger in the cry1 cry2 double mutant. Slow rate of curvature at low fluence rates of blue light in cry1 cry2 (PubMed:12857830). Lower anthocyanin accumulation in the phyB cry1 double mutant exposed to far-red light. Reduced chlorophyll levels in the phyB cry1 double mutant exposed to red light. In blue light, impaired cotyledon unfolding and smaller cotyledons, longer hypocotyls and less chlorophyll (PubMed:9733523). Impaired accumulation of reactive oxygen species (ROS) in double mutant cry1 cry2 exposed to high-intensity blue light (PubMed:25728686). Altered blue-light-triggered and singlet oxygen-mediated programmed cell death (PCD) (PubMed:17075038). The double mutant cry1 cry2 exhibits a reduced impact of near-null magnetic field on flowering in lower blue light intensity and short days (PubMed:26095447). Reduced hyponastic growth (differential growth-driven upward leaf movement) in low blue light fluence (PubMed:19558423). The double mutant cry1 cry2 is hyposensitive to the strigolactone analog GR24 (PubMed:24126495). The mutant cry1 exposed to a background of red light show severely impaired stomatal opening responses to blue light. The double mutant cry1 cry2 has reduced stomatal conductance, transpiration, and photosynthesis, particularly under the high irradiance of full sunlight at midday, associated with elevated abscisic acid levels (PubMed:22147516). The cry1 mutants grown in complete darkness have premature opening of the hypocotyl hook (PubMed:22855128). Reduced expression of nuclear genes encoding photoprotective components in response to extreme high light (PubMed:22786870). Reduced shade avoidance syndrome (SAS) when exposed to blue light attenuation (PubMed:21457375). Reduced growth at warm ambient temperatures (PubMed:21265897). Down-regulated local resistance and systemic acquired resistance (SAR) to Pseudomonas syringae pv. tomato (Pst.) DC3000 under continuous light conditions, leading to pathogen proliferation (PubMed:20053798). When grown in blue light, increased growth of lateral roots and reduced sensitivity to auxin (IAA) on this phenotype (PubMed:20133010).23 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi21D → N in cry1-401; genomes uncoupled mutant (gun) with defects in plastid-to-nucleus signaling. 1 Publication1
Mutagenesisi66S → N: Loss of dimerization and activity. Abnormal hypocotyl elongation in blue light. 2 Publications1
Mutagenesisi220G → D in hy4-6; reduced anthocyanin accumulation and abnormal hypocotyl elongation in blue light. 2 Publications1
Mutagenesisi283G → E in hy4-5; reduced anthocyanin accumulation and abnormal hypocotyl elongation in blue light. 2 Publications1
Mutagenesisi286S → N in cry1-402; genomes uncoupled mutant (gun) with defects in plastid-to-nucleus signaling. 1 Publication1
Mutagenesisi324W → F: Impaired photoreduction in vitro, but not in vivo or in whole cell extracts, due to an alternative electron transport that involves small metabolites. Abolished intra-protein electron transfer cascade and impaired conformational change upon photoexcitation. 2 Publications1
Mutagenesisi337G → D: Abnormal hypocotyl elongation in blue light. 1 Publication1
Mutagenesisi340G → E in cry1-404 and hy4-1; reduced anthocyanin accumulation and abnormal hypocotyl elongation in blue light. Loss of activity. Genomes uncoupled mutant (gun) with defects in plastid-to-nucleus signaling. 4 Publications1
Mutagenesisi347G → E in hy4-16; reduced anthocyanin accumulation and abnormal hypocotyl elongation in blue light. 2 Publications1
Mutagenesisi347G → R in hy4-15; reduced anthocyanin accumulation and abnormal hypocotyl elongation in blue light. Loss of dimerization and activity. 3 Publications1
Mutagenesisi380G → R: Constitutive light response. 1 Publication1
Mutagenesisi396D → N: Upon illumination, formation of the reduced anionic flavin (RED) flavin, useful for DNA repair, rather than the semi-reduced radical form (SR) flavin, which is correlated with cryptochrome activity. 1 Publication1
Mutagenesisi400W → F: Impaired photoreduction in vitro, but not in vivo or whole cell extracts, due to an alternative electron transport that involves small metabolites. 1 Publication1
Mutagenesisi407L → F: Gain of function mutant. Hypersensitive toward blue, red, and far-red light in hypocotyl growth inhibition. Very early flowering in short-day conditions, associated with enhanced expression of CO and FT. Impaired interaction with PHYB. 2 Publications1
Mutagenesisi462A → V: Loss of dimerization and activity. Abnormal hypocotyl elongation in blue light. 2 Publications1
Mutagenesisi515E → K in hy4-19; reduced anthocyanin accumulation and abnormal hypocotyl elongation in blue light. 1 Publication1
Mutagenesisi531E → K in hy4-20; reduced anthocyanin accumulation and abnormal hypocotyl elongation in blue light. 1 Publication1
Mutagenesisi549P → L in hy4-9; reduced anthocyanin accumulation and abnormal hypocotyl elongation in blue light. 1 Publication1
Mutagenesisi559E → K in hy4-22; reduced anthocyanin accumulation and abnormal hypocotyl elongation in blue light. 1 Publication1
Mutagenesisi576R → K in hy4-10; reduced anthocyanin accumulation and abnormal hypocotyl elongation in blue light. 1 Publication1
Mutagenesisi581R → K in hy4-23; reduced anthocyanin accumulation and abnormal hypocotyl elongation in blue light. 1 Publication1
Mutagenesisi611R → K in hy4-24; reduced anthocyanin accumulation and abnormal hypocotyl elongation in blue light. 1 Publication1
Mutagenesisi623E → K in cry1-403; genomes uncoupled mutant (gun) with defects in plastid-to-nucleus signaling. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000851211 – 681Cryptochrome-1Add BLAST681

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi80 ↔ 190Combined sources1 Publication
Modified residuei616PhosphoserineBy similarity1
Modified residuei621PhosphothreonineBy similarity1

Post-translational modificationi

Autophosphorylated; in response to blue light and when in complex with FAD cofactor (PubMed:12846824, PubMed:14523249, PubMed:9651577, PubMed:17073458). Kinase activity is optimal in the presence of magnesium ions, about 30 percent of the optimal activity in the presence of manganese ions, but inactive with calcium ions (PubMed:17073458). Adopts an open conformation when phosphorylated upon photoexcitation and thus interacts with signaling partner proteins (PubMed:21841916).1 Publication4 Publications

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ43125.

PTM databases

iPTMnetiQ43125.

Expressioni

Tissue specificityi

Widely expressed (PubMed:8953250). Expressed in the aerial tissues (e.g. cotyledons and leaf primordia), but not detected in the roots (PubMed:11743105).2 Publications

Inductioni

Expression levels display circadian oscillations under constant conditions, with a high amplitude and an early phase, with maximal expression around 4-6 hours of the light phase. Induced by light (PubMed:11743105). Transcripts levels oscillate weakly and proportionally to temperature, but protein levels are stable, with higher levels at low temperature (12 degrees Celsius) (PubMed:23511208). Accumulates in response to low blue light (LBL) (PubMed:26724867).3 Publications

Gene expression databases

GenevisibleiQ43125. AT.

Interactioni

Subunit structurei

Homodimer. Interacts with ADO1, COP1 and PHYA. Interacts specifically with the dark/far-red (Pr) state of PHYB, but not with the red light-activated (Pfr) (PubMed:22577138). Interacts with PIF4 and PIF5 in the nucleus in response to low blue light (LBL) (PubMed:26724867). Binds to SPA1 and SPA4 in response to blue light, this interaction prevents SPA1/COP1 complex formation and thus avoid COP1-dependent degradation of the transcription factor HY5 by the proteasome and promotes hypocotyl elongation (PubMed:21511872, PubMed:21511871). Interacts with TCP2 (PubMed:26596765). Binding to ATP mediates conformational changes which facilitate flavin binding (PubMed:19327354, PubMed:17073458).13 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-300703,EBI-300703
COP1P432544EBI-300703,EBI-301649
PHYA3P065932EBI-300703,EBI-630413From a different organism.

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi11769. 7 interactors.
IntActiQ43125. 3 interactors.
MINTiMINT-192438.
STRINGi3702.AT4G08920.1.

Structurei

Secondary structure

1681
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 20Combined sources7
Beta strandi24 – 26Combined sources3
Helixi28 – 36Combined sources9
Beta strandi39 – 45Combined sources7
Helixi47 – 50Combined sources4
Helixi57 – 76Combined sources20
Beta strandi81 – 85Combined sources5
Helixi89 – 100Combined sources12
Beta strandi104 – 108Combined sources5
Helixi113 – 127Combined sources15
Turni128 – 130Combined sources3
Beta strandi132 – 136Combined sources5
Helixi144 – 146Combined sources3
Beta strandi150 – 152Combined sources3
Helixi158 – 166Combined sources9
Helixi187 – 189Combined sources3
Helixi200 – 206Combined sources7
Helixi209 – 212Combined sources4
Helixi217 – 228Combined sources12
Helixi231 – 234Combined sources4
Turni235 – 240Combined sources6
Beta strandi242 – 244Combined sources3
Helixi251 – 255Combined sources5
Helixi261 – 278Combined sources18
Helixi281 – 305Combined sources25
Turni308 – 312Combined sources5
Turni318 – 321Combined sources4
Helixi328 – 336Combined sources9
Helixi342 – 354Combined sources13
Helixi359 – 371Combined sources13
Helixi377 – 387Combined sources11
Helixi393 – 404Combined sources12
Helixi419 – 426Combined sources8
Helixi431 – 436Combined sources6
Helixi438 – 440Combined sources3
Helixi445 – 448Combined sources4
Turni451 – 453Combined sources3
Helixi456 – 462Combined sources7
Turni467 – 469Combined sources3
Helixi477 – 495Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U3CX-ray2.60A1-509[»]
1U3DX-ray2.45A1-509[»]
DisProtiDP00474.
ProteinModelPortaliQ43125.
SMRiQ43125.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ43125.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 141Photolyase/cryptochrome alpha/betaSequence analysisAdd BLAST130

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 489CNT1, binds chromophores to sense blue light and mediate CRY dimerization1 PublicationAdd BLAST489
Regioni490 – 681CCT1/CCE1, mediates blue light signaling1 Publication1 PublicationAdd BLAST192

Domaini

The N-terminal domain CNT1 (1-489) is sufficient for autophosphorylation and is required for dimerization. The C-terminal domain CCT1 (490-681) of the homodimer binds to COP1.

Sequence similaritiesi

Belongs to the DNA photolyase class-1 family.Curated
Contains 1 photolyase/cryptochrome alpha/beta domain.Sequence analysis

Phylogenomic databases

eggNOGiKOG0133. Eukaryota.
COG0415. LUCA.
HOGENOMiHOG000245621.
InParanoidiQ43125.
KOiK12118.
OMAiIFFNHLY.
OrthoDBiEOG0936047F.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR005101. Cryptochr/Photolyase_FAD-bd.
IPR002081. Cryptochrome/DNA_photolyase_1.
IPR020978. Cryptochrome_C.
IPR014134. Cryptochrome_pln.
IPR018394. DNA_photolyase_1_CS_C.
IPR006050. DNA_photolyase_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF12546. Cryptochrome_C. 1 hit.
PF00875. DNA_photolyase. 1 hit.
PF03441. FAD_binding_7. 1 hit.
[Graphical view]
PRINTSiPR00147. DNAPHOTLYASE.
SUPFAMiSSF48173. SSF48173. 1 hit.
SSF52425. SSF52425. 1 hit.
TIGRFAMsiTIGR02766. crypt_chrom_pln. 1 hit.
PROSITEiPS00394. DNA_PHOTOLYASES_1_1. 1 hit.
PS00691. DNA_PHOTOLYASES_1_2. 1 hit.
PS51645. PHR_CRY_ALPHA_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q43125-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGSVSGCGS GGCSIVWFRR DLRVEDNPAL AAAVRAGPVI ALFVWAPEEE
60 70 80 90 100
GHYHPGRVSR WWLKNSLAQL DSSLRSLGTC LITKRSTDSV ASLLDVVKST
110 120 130 140 150
GASQIFFNHL YDPLSLVRDH RAKDVLTAQG IAVRSFNADL LYEPWEVTDE
160 170 180 190 200
LGRPFSMFAA FWERCLSMPY DPESPLLPPK KIISGDVSKC VADPLVFEDD
210 220 230 240 250
SEKGSNALLA RAWSPGWSNG DKALTTFING PLLEYSKNRR KADSATTSFL
260 270 280 290 300
SPHLHFGEVS VRKVFHLVRI KQVAWANEGN EAGEESVNLF LKSIGLREYS
310 320 330 340 350
RYISFNHPYS HERPLLGHLK FFPWAVDENY FKAWRQGRTG YPLVDAGMRE
360 370 380 390 400
LWATGWLHDR IRVVVSSFFV KVLQLPWRWG MKYFWDTLLD ADLESDALGW
410 420 430 440 450
QYITGTLPDS REFDRIDNPQ FEGYKFDPNG EYVRRWLPEL SRLPTDWIHH
460 470 480 490 500
PWNAPESVLQ AAGIELGSNY PLPIVGLDEA KARLHEALSQ MWQLEAASRA
510 520 530 540 550
AIENGSEEGL GDSAEVEEAP IEFPRDITME ETEPTRLNPN RRYEDQMVPS
560 570 580 590 600
ITSSLIRPEE DEESSLNLRN SVGDSRAEVP RNMVNTNQAQ QRRAEPASNQ
610 620 630 640 650
VTAMIPEFNI RIVAESTEDS TAESSSSGRR ERSGGIVPEW SPGYSEQFPS
660 670 680
EENGIGGGST TSSYLQNHHE ILNWRRLSQT G
Length:681
Mass (Da):76,695
Last modified:May 3, 2011 - v2
Checksum:i372A7E6DDC2AC076
GO

Sequence cautioni

The sequence AAB28725 differs from that shown. Reason: Frameshift at position 546.Curated
The sequence AAD17364 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB78016 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40I → N in AAK32756 (PubMed:14593172).Curated1
Sequence conflicti654G → R in AAB28724 (PubMed:8232555).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S66907 mRNA. Translation: AAB28724.1.
S66909 mRNA. Translation: AAB28725.2. Frameshift.
AF128396 Genomic DNA. Translation: AAD17364.1. Sequence problems.
AL161513 Genomic DNA. Translation: CAB78016.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82696.1.
AF361588 mRNA. Translation: AAK32756.1.
AY124863 mRNA. Translation: AAM70572.1.
PIRiH85089.
S39058.
RefSeqiNP_567341.1. NM_116961.5.
UniGeneiAt.27730.

Genome annotation databases

EnsemblPlantsiAT4G08920.1; AT4G08920.1; AT4G08920.
GeneIDi826470.
GrameneiAT4G08920.1; AT4G08920.1; AT4G08920.
KEGGiath:AT4G08920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S66907 mRNA. Translation: AAB28724.1.
S66909 mRNA. Translation: AAB28725.2. Frameshift.
AF128396 Genomic DNA. Translation: AAD17364.1. Sequence problems.
AL161513 Genomic DNA. Translation: CAB78016.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE82696.1.
AF361588 mRNA. Translation: AAK32756.1.
AY124863 mRNA. Translation: AAM70572.1.
PIRiH85089.
S39058.
RefSeqiNP_567341.1. NM_116961.5.
UniGeneiAt.27730.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U3CX-ray2.60A1-509[»]
1U3DX-ray2.45A1-509[»]
DisProtiDP00474.
ProteinModelPortaliQ43125.
SMRiQ43125.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi11769. 7 interactors.
IntActiQ43125. 3 interactors.
MINTiMINT-192438.
STRINGi3702.AT4G08920.1.

PTM databases

iPTMnetiQ43125.

Proteomic databases

PaxDbiQ43125.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G08920.1; AT4G08920.1; AT4G08920.
GeneIDi826470.
GrameneiAT4G08920.1; AT4G08920.1; AT4G08920.
KEGGiath:AT4G08920.

Organism-specific databases

TAIRiAT4G08920.

Phylogenomic databases

eggNOGiKOG0133. Eukaryota.
COG0415. LUCA.
HOGENOMiHOG000245621.
InParanoidiQ43125.
KOiK12118.
OMAiIFFNHLY.
OrthoDBiEOG0936047F.

Enzyme and pathway databases

BioCyciARA:GQT-2162-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ43125.
PROiQ43125.

Gene expression databases

GenevisibleiQ43125. AT.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR005101. Cryptochr/Photolyase_FAD-bd.
IPR002081. Cryptochrome/DNA_photolyase_1.
IPR020978. Cryptochrome_C.
IPR014134. Cryptochrome_pln.
IPR018394. DNA_photolyase_1_CS_C.
IPR006050. DNA_photolyase_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF12546. Cryptochrome_C. 1 hit.
PF00875. DNA_photolyase. 1 hit.
PF03441. FAD_binding_7. 1 hit.
[Graphical view]
PRINTSiPR00147. DNAPHOTLYASE.
SUPFAMiSSF48173. SSF48173. 1 hit.
SSF52425. SSF52425. 1 hit.
TIGRFAMsiTIGR02766. crypt_chrom_pln. 1 hit.
PROSITEiPS00394. DNA_PHOTOLYASES_1_1. 1 hit.
PS00691. DNA_PHOTOLYASES_1_2. 1 hit.
PS51645. PHR_CRY_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRY1_ARATH
AccessioniPrimary (citable) accession number: Q43125
Secondary accession number(s): Q43126
, Q8L7Y1, Q9ASZ2, Q9M0S9, Q9ZPF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 3, 2011
Last modified: November 30, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a DNA photolyase.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.