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Reviewed, UniProtKB/Swiss-Prot Q43125 (CRY1_ARATH)

Last modified November 25, 2008. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cryptochrome-1
Alternative name(s):
    Blue light photoreceptor
    Protein OUT OF PHASE 2
      Short name=OOP2
    Protein BLUE LIGHT UNINHIBITED 1
    Protein ELONGATED HYPOCOTYL 4
Gene names
Name: CRY1
Synonyms: BLU1, HY4
Ordered Locus Names: At4g08920
ORF Names: T3H13.5, T3H13.14
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length681 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Mediates blue light-induced gene expression through the inhibition of COP1-mediated degradation of the transcription factor BIT1. Involved in blue light-dependent stomatal opening, CHS gene expression, inhibition of stem growth and increase of root growth.

Cofactor

Binds 1 FAD per subunit.

Binds 1 5,10-methenyltetrahydrofolate (MTHF) per subunit.

Enzyme regulation

Light exposure induces a conformational change in the C-terminal domain CCT1 required for activity.

Subunit structure

Homodimer. Interacts with ADO1, COP1 and PHYA.

Subcellular location

Cytoplasm. Nucleus. Note= The nuclear pool is involved in hypocotyl and petiole growth inhibition and anthocyanin production, while the cytoplasmic pool is involved in root growth and cotyledon expansion.

Tissue specificity

Widely expressed.

Domain

The N-terminal domain CNT1 (1-489) is sufficient for autophosphorylation and is required for dimerization. The C-terminal domain CCT1 (490-681) of the homodimer binds to COP1.

Post-translational modification

Autophosphorylated; in response to blue light.

Miscellaneous

Plants lacking CRY1 show reduced root elongation in blue light.

Sequence similarities

Belongs to the DNA photolyase class-1 family.

Contains 1 DNA photolyase domain.

Caution

Was originally (Ref.1) thought to be a DNA photolyase.

Sequence caution

The sequence AAB28725.2 differs from that shown. Reason: Frameshift at position 546.

The sequence AAD17364.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB78016.1 differs from that shown. Reason: Erroneous gene model prediction.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself3EBI-300703,EBI-300703
ADO1Q94BT61EBI-300703,EBI-300691

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 681681Cryptochrome-1
PRO_0000085121

Regions

Nucleotide binding247 – 2515FAD

Sites

Binding site2351FAD
Binding site3591ATP
Binding site3591FAD
Binding site3601ATP
Binding site3901FAD; via carbonyl oxygen
Binding site3921FAD; via amide nitrogen
Binding site4091ATP

Amino acid modifications

Disulfide bond80 ↔ 190

Experimental info

Mutagenesis661S → N: Loss of dimerization and activity
Mutagenesis3401G → E: Loss of activity
Mutagenesis3471G → R: Loss of dimerization and activity
Mutagenesis4621A → V: Loss of dimerization and activity
Sequence conflict401I → N in AAK32756. Ref.3
Sequence conflict6541R → G in AAM70572. Ref.3

Secondary structure

............................................................................. 681
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q43125-1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 372A7E798D6FC076

FASTA68176,794
        10         20         30         40         50         60 
MSGSVSGCGS GGCSIVWFRR DLRVEDNPAL AAAVRAGPVI ALFVWAPEEE GHYHPGRVSR 

        70         80         90        100        110        120 
WWLKNSLAQL DSSLRSLGTC LITKRSTDSV ASLLDVVKST GASQIFFNHL YDPLSLVRDH 

       130        140        150        160        170        180 
RAKDVLTAQG IAVRSFNADL LYEPWEVTDE LGRPFSMFAA FWERCLSMPY DPESPLLPPK 

       190        200        210        220        230        240 
KIISGDVSKC VADPLVFEDD SEKGSNALLA RAWSPGWSNG DKALTTFING PLLEYSKNRR 

       250        260        270        280        290        300 
KADSATTSFL SPHLHFGEVS VRKVFHLVRI KQVAWANEGN EAGEESVNLF LKSIGLREYS 

       310        320        330        340        350        360 
RYISFNHPYS HERPLLGHLK FFPWAVDENY FKAWRQGRTG YPLVDAGMRE LWATGWLHDR 

       370        380        390        400        410        420 
IRVVVSSFFV KVLQLPWRWG MKYFWDTLLD ADLESDALGW QYITGTLPDS REFDRIDNPQ 

       430        440        450        460        470        480 
FEGYKFDPNG EYVRRWLPEL SRLPTDWIHH PWNAPESVLQ AAGIELGSNY PLPIVGLDEA 

       490        500        510        520        530        540 
KARLHEALSQ MWQLEAASRA AIENGSEEGL GDSAEVEEAP IEFPRDITME ETEPTRLNPN 

       550        560        570        580        590        600 
RRYEDQMVPS ITSSLIRPEE DEESSLNLRN SVGDSRAEVP RNMVNTNQAQ QRRAEPASNQ 

       610        620        630        640        650        660 
VTAMIPEFNI RIVAESTEDS TAESSSSGRR ERSGGIVPEW SPGYSEQFPS EENRIGGGST 

       670        680 
TSSYLQNHHE ILNWRRLSQT G 

« Hide

References

« Hide 'large scale' references
[1]"HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor."
Ahmad M., Cashmore A.R.
Nature 366:162-166(1993) [PubMed: 8232555] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed: 10617198] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.