ID   KPYA_RICCO              Reviewed;         583 AA.
AC   Q43117; Q43118;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 63.
DE   RecName: Full=Pyruvate kinase isozyme A, chloroplastic;
DE            EC=2.7.1.40;
DE   Flags: Precursor;
OS   Ricinus communis (Castor bean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Malpighiales; Euphorbiaceae; Acalyphoideae;
OC   Acalypheae; Ricinus.
OX   NCBI_TaxID=3988;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA), AND PROTEIN
RP   SEQUENCE OF 75-81.
RC   STRAIN=cv. Baker 296; TISSUE=Endosperm;
RX   PubMed=16668331;
RA   Blakeley S.D., Plaxton W.C., Dennis D.T.;
RT   "Relationship between the subunits of leucoplast pyruvate kinase from
RT   Ricinus communis and a comparison with the enzyme from other
RT   sources.";
RL   Plant Physiol. 96:1283-1288(1991).
CC   -!- CATALYTIC ACTIVITY: ATP + pyruvate = ADP + phosphoenolpyruvate.
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- COFACTOR: Potassium (By similarity).
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 5/5.
CC   -!- SUBUNIT: Oligomer of alpha and beta subunits (By similarity).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=Alpha;
CC         IsoId=Q43117-1; Sequence=Displayed;
CC       Name=Beta;
CC         IsoId=Q43117-2; Sequence=VSP_002886;
CC   -!- SIMILARITY: Belongs to the pyruvate kinase family.
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DR   EMBL; M64736; AAA33870.1; -; mRNA.
DR   EMBL; M64737; AAA33871.1; -; mRNA.
DR   PIR; T10051; T10051.
DR   PIR; T10054; T10054.
DR   HSSP; P14178; 1E0T.
DR   BRENDA; 2.7.1.40; 816.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030955; F:potassium ion binding; IEA:InterPro.
DR   GO; GO:0004743; F:pyruvate kinase activity; IEA:EC.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR001697; Pyr_Knase.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase_cat.
DR   InterPro; IPR015794; Pyrv_Knase_a/b.
DR   InterPro; IPR018209; Pyrv_Knase_AS.
DR   InterPro; IPR015793; Pyrv_Knase_brl.
DR   Gene3D; G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 1.
DR   Gene3D; G3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1.
DR   PANTHER; PTHR11817; Pyruvate_kinase; 1.
DR   Pfam; PF00224; PK; 1.
DR   Pfam; PF02887; PK_C; 1.
DR   PRINTS; PR01050; PYRUVTKNASE.
DR   ProDom; PD001009; Pyruvate_kinase; 2.
DR   TIGRFAMs; TIGR01064; pyruv_kin; 1.
DR   PROSITE; PS00110; PYRUVATE_KINASE; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; ATP-binding; Chloroplast;
KW   Direct protein sequencing; Glycolysis; Kinase; Magnesium;
KW   Metal-binding; Nucleotide-binding; Plastid; Pyruvate; Transferase;
KW   Transit peptide.
FT   TRANSIT       1     74       Chloroplast.
FT   CHAIN        75    583       Pyruvate kinase isozyme A, chloroplastic.
FT                                /FTId=PRO_0000016662.
FT   ACT_SITE    331    331       By similarity.
FT   METAL       333    333       Magnesium (Potential).
FT   METAL       354    354       Magnesium (Potential).
FT   METAL       355    355       Magnesium (Potential).
FT   VAR_SEQ       1    129       MSQSLHFSPNLTFAKQPFPKLPLPFPTSNSRYPVNNYKSLS
FT                                IKASTSPSSSSDPQVLVADNGTGNSGVLYNNNNKSVTVSDP
FT                                SSIEVDAVTETELKENGFRSTRRTKLVCTIGPATCGFEELE
FT                                ALAVGG -> MAVVVKDLEEAVRVVVLAVLRDMEVVVVLVT
FT                                AVMGVVGD (in isoform Beta).
FT                                /FTId=VSP_002886.
SQ   SEQUENCE   583 AA;  64094 MW;  5DFDE4F778A753C2 CRC64;
     MSQSLHFSPN LTFAKQPFPK LPLPFPTSNS RYPVNNYKSL SIKASTSPSS SSDPQVLVAD
     NGTGNSGVLY NNNNKSVTVS DPSSIEVDAV TETELKENGF RSTRRTKLVC TIGPATCGFE
     ELEALAVGGM NVARINMCHG TREWHKSVIE RVRRLNEEKG FAVAIMMDTE GSEIHMGDLG
     GASSAKAEDG EIWTFSVRAY DSPRPERTIN VNYDGFAEDV KVGDELLVDG GMVRFEVIEK
     IGPDVKCRCT DPGLLLPRAN LTFWRDGSLV RERNAMLPTI SSKDWLDIDF GIAEGVDFIA
     ISFVKSAEVI NHLKSYIAAR SRDSDIAVIA KIESIDSLKN LEEIIRASDG AMVARGDLGA
     QIPLEQVPSA QQNIVQVCRQ LNKPVIVASQ LLESMIEYPT PTRAEVADVS EAVRQRADAL
     MLSGESAMGQ YPEKALAVLR SVSVRIEKWW REEKHHEAME LPAIGSTYSD SISEEICNSA
     AKMANNLGVD ALFVYTKDGH MASLLSRCRP DCPIFAFTTT TSVRRRLNLQ WGLIPFRLSF
     ADDMESNLNK TFSLLKARGM IKSGDLVIAV SDMLQSIQVM NVP
//