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Q43117

- KPYA_RICCO

UniProt

Q43117 - KPYA_RICCO

Protein

Pyruvate kinase isozyme A, chloroplastic

Gene
N/A
Organism
Ricinus communis (Castor bean)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei134 – 1341SubstrateBy similarity
    Metal bindingi136 – 1361PotassiumBy similarity
    Metal bindingi168 – 1681PotassiumBy similarity
    Metal bindingi169 – 1691Potassium; via carbonyl oxygenBy similarity
    Sitei331 – 3311Transition state stabilizerBy similarity
    Metal bindingi333 – 3331MagnesiumSequence Analysis
    Binding sitei356 – 3561Substrate; via amide nitrogenBy similarity
    Metal bindingi357 – 3571MagnesiumBy similarity
    Binding sitei357 – 3571Substrate; via amide nitrogenBy similarity
    Binding sitei389 – 3891SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    SABIO-RKQ43117.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase isozyme A, chloroplastic (EC:2.7.1.40)
    OrganismiRicinus communis (Castor bean)
    Taxonomic identifieri3988 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeAcalyphoideaeAcalypheaeRicinus

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 7474Chloroplast1 PublicationAdd
    BLAST
    Chaini75 – 583509Pyruvate kinase isozyme A, chloroplasticPRO_0000016662Add
    BLAST

    Proteomic databases

    PRIDEiQ43117.

    Interactioni

    Subunit structurei

    Oligomer of alpha and beta subunits.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ43117.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK00873.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Alpha (identifier: Q43117-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSQSLHFSPN LTFAKQPFPK LPLPFPTSNS RYPVNNYKSL SIKASTSPSS    50
    SSDPQVLVAD NGTGNSGVLY NNNNKSVTVS DPSSIEVDAV TETELKENGF 100
    RSTRRTKLVC TIGPATCGFE ELEALAVGGM NVARINMCHG TREWHKSVIE 150
    RVRRLNEEKG FAVAIMMDTE GSEIHMGDLG GASSAKAEDG EIWTFSVRAY 200
    DSPRPERTIN VNYDGFAEDV KVGDELLVDG GMVRFEVIEK IGPDVKCRCT 250
    DPGLLLPRAN LTFWRDGSLV RERNAMLPTI SSKDWLDIDF GIAEGVDFIA 300
    ISFVKSAEVI NHLKSYIAAR SRDSDIAVIA KIESIDSLKN LEEIIRASDG 350
    AMVARGDLGA QIPLEQVPSA QQNIVQVCRQ LNKPVIVASQ LLESMIEYPT 400
    PTRAEVADVS EAVRQRADAL MLSGESAMGQ YPEKALAVLR SVSVRIEKWW 450
    REEKHHEAME LPAIGSTYSD SISEEICNSA AKMANNLGVD ALFVYTKDGH 500
    MASLLSRCRP DCPIFAFTTT TSVRRRLNLQ WGLIPFRLSF ADDMESNLNK 550
    TFSLLKARGM IKSGDLVIAV SDMLQSIQVM NVP 583
    Length:583
    Mass (Da):64,094
    Last modified:November 1, 1996 - v1
    Checksum:i5DFDE4F778A753C2
    GO
    Isoform Beta (identifier: Q43117-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-129: MSQSLHFSPN...EELEALAVGG → MAVVVKDLEE...VTAVMGVVGD

    Show »
    Length:493
    Mass (Da):54,462
    Checksum:i375858858BF6124F
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 129129MSQSL…LAVGG → MAVVVKDLEEAVRVVVLAVL RDMEVVVVLVTAVMGVVGD in isoform Beta. 1 PublicationVSP_002886Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64736 mRNA. Translation: AAA33870.1.
    M64737 mRNA. Translation: AAA33871.1.
    PIRiT10051.
    T10054.
    RefSeqiXP_002517044.1. XM_002516998.1. [Q43117-1]

    Genome annotation databases

    GeneIDi8280200.
    KEGGircu:RCOM_0910580.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64736 mRNA. Translation: AAA33870.1 .
    M64737 mRNA. Translation: AAA33871.1 .
    PIRi T10051.
    T10054.
    RefSeqi XP_002517044.1. XM_002516998.1. [Q43117-1 ]

    3D structure databases

    ProteinModelPortali Q43117.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q43117.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 8280200.
    KEGGi rcu:RCOM_0910580.

    Phylogenomic databases

    KOi K00873.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    SABIO-RK Q43117.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Relationship between the subunits of leucoplast pyruvate kinase from Ricinus communis and a comparison with the enzyme from other sources."
      Blakeley S.D., Plaxton W.C., Dennis D.T.
      Plant Physiol. 96:1283-1288(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA), PROTEIN SEQUENCE OF 75-81.
      Strain: cv. Baker 296.
      Tissue: Endosperm.

    Entry informationi

    Entry nameiKPYA_RICCO
    AccessioniPrimary (citable) accession number: Q43117
    Secondary accession number(s): Q43118
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3