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Q43117 (KPYA_RICCO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase isozyme A, chloroplastic

EC=2.7.1.40
OrganismRicinus communis (Castor bean)
Taxonomic identifier3988 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeAcalyphoideaeAcalypheaeRicinus

Protein attributes

Sequence length583 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Oligomer of alpha and beta subunits By similarity.

Subcellular location

Plastidchloroplast.

Sequence similarities

Belongs to the pyruvate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentChloroplast
Plastid
   Coding sequence diversityAlternative splicing
   DomainTransit peptide
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
Pyruvate
   Molecular functionKinase
Transferase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

potassium ion binding

Inferred from electronic annotation. Source: InterPro

pyruvate kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha (identifier: Q43117-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta (identifier: Q43117-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-129: MSQSLHFSPN...EELEALAVGG → MAVVVKDLEE...VTAVMGVVGD

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 7474Chloroplast Ref.1
Chain75 – 583509Pyruvate kinase isozyme A, chloroplastic
PRO_0000016662

Sites

Metal binding1361Potassium By similarity
Metal binding1681Potassium By similarity
Metal binding1691Potassium; via carbonyl oxygen By similarity
Metal binding3331Magnesium Potential
Metal binding3571Magnesium By similarity
Binding site1341Substrate By similarity
Binding site3561Substrate; via amide nitrogen By similarity
Binding site3571Substrate; via amide nitrogen By similarity
Binding site3891Substrate By similarity
Site3311Transition state stabilizer By similarity

Natural variations

Alternative sequence1 – 129129MSQSL…LAVGG → MAVVVKDLEEAVRVVVLAVL RDMEVVVVLVTAVMGVVGD in isoform Beta.
VSP_002886

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 5DFDE4F778A753C2

FASTA58364,094
        10         20         30         40         50         60 
MSQSLHFSPN LTFAKQPFPK LPLPFPTSNS RYPVNNYKSL SIKASTSPSS SSDPQVLVAD 

        70         80         90        100        110        120 
NGTGNSGVLY NNNNKSVTVS DPSSIEVDAV TETELKENGF RSTRRTKLVC TIGPATCGFE 

       130        140        150        160        170        180 
ELEALAVGGM NVARINMCHG TREWHKSVIE RVRRLNEEKG FAVAIMMDTE GSEIHMGDLG 

       190        200        210        220        230        240 
GASSAKAEDG EIWTFSVRAY DSPRPERTIN VNYDGFAEDV KVGDELLVDG GMVRFEVIEK 

       250        260        270        280        290        300 
IGPDVKCRCT DPGLLLPRAN LTFWRDGSLV RERNAMLPTI SSKDWLDIDF GIAEGVDFIA 

       310        320        330        340        350        360 
ISFVKSAEVI NHLKSYIAAR SRDSDIAVIA KIESIDSLKN LEEIIRASDG AMVARGDLGA 

       370        380        390        400        410        420 
QIPLEQVPSA QQNIVQVCRQ LNKPVIVASQ LLESMIEYPT PTRAEVADVS EAVRQRADAL 

       430        440        450        460        470        480 
MLSGESAMGQ YPEKALAVLR SVSVRIEKWW REEKHHEAME LPAIGSTYSD SISEEICNSA 

       490        500        510        520        530        540 
AKMANNLGVD ALFVYTKDGH MASLLSRCRP DCPIFAFTTT TSVRRRLNLQ WGLIPFRLSF 

       550        560        570        580 
ADDMESNLNK TFSLLKARGM IKSGDLVIAV SDMLQSIQVM NVP 

« Hide

Isoform Beta [UniParc].

Checksum: 375858858BF6124F
Show »

FASTA49354,462

References

[1]"Relationship between the subunits of leucoplast pyruvate kinase from Ricinus communis and a comparison with the enzyme from other sources."
Blakeley S.D., Plaxton W.C., Dennis D.T.
Plant Physiol. 96:1283-1288(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA), PROTEIN SEQUENCE OF 75-81.
Strain: cv. Baker 296.
Tissue: Endosperm.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M64736 mRNA. Translation: AAA33870.1.
M64737 mRNA. Translation: AAA33871.1.
PIRT10051.
T10054.
RefSeqXP_002517044.1. XM_002516998.1. [Q43117-1]

3D structure databases

ProteinModelPortalQ43117.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ43117.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8280200.
KEGGrcu:RCOM_0910580.

Phylogenomic databases

KOK00873.

Enzyme and pathway databases

SABIO-RKQ43117.
UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYA_RICCO
AccessionPrimary (citable) accession number: Q43117
Secondary accession number(s): Q43118
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways