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Q43117

- KPYA_RICCO

UniProt

Q43117 - KPYA_RICCO

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Protein

Pyruvate kinase isozyme A, chloroplastic

Gene
N/A
Organism
Ricinus communis (Castor bean)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.By similarity
Potassium.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei134 – 1341SubstrateBy similarity
Metal bindingi136 – 1361PotassiumBy similarity
Metal bindingi168 – 1681PotassiumBy similarity
Metal bindingi169 – 1691Potassium; via carbonyl oxygenBy similarity
Sitei331 – 3311Transition state stabilizerBy similarity
Metal bindingi333 – 3331MagnesiumSequence Analysis
Binding sitei356 – 3561Substrate; via amide nitrogenBy similarity
Metal bindingi357 – 3571MagnesiumBy similarity
Binding sitei357 – 3571Substrate; via amide nitrogenBy similarity
Binding sitei389 – 3891SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

SABIO-RKQ43117.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase isozyme A, chloroplastic (EC:2.7.1.40)
OrganismiRicinus communis (Castor bean)
Taxonomic identifieri3988 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeAcalyphoideaeAcalypheaeRicinus

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7474Chloroplast1 PublicationAdd
BLAST
Chaini75 – 583509Pyruvate kinase isozyme A, chloroplasticPRO_0000016662Add
BLAST

Proteomic databases

PRIDEiQ43117.

Interactioni

Subunit structurei

Oligomer of alpha and beta subunits.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ43117.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK00873.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Alpha (identifier: Q43117-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQSLHFSPN LTFAKQPFPK LPLPFPTSNS RYPVNNYKSL SIKASTSPSS
60 70 80 90 100
SSDPQVLVAD NGTGNSGVLY NNNNKSVTVS DPSSIEVDAV TETELKENGF
110 120 130 140 150
RSTRRTKLVC TIGPATCGFE ELEALAVGGM NVARINMCHG TREWHKSVIE
160 170 180 190 200
RVRRLNEEKG FAVAIMMDTE GSEIHMGDLG GASSAKAEDG EIWTFSVRAY
210 220 230 240 250
DSPRPERTIN VNYDGFAEDV KVGDELLVDG GMVRFEVIEK IGPDVKCRCT
260 270 280 290 300
DPGLLLPRAN LTFWRDGSLV RERNAMLPTI SSKDWLDIDF GIAEGVDFIA
310 320 330 340 350
ISFVKSAEVI NHLKSYIAAR SRDSDIAVIA KIESIDSLKN LEEIIRASDG
360 370 380 390 400
AMVARGDLGA QIPLEQVPSA QQNIVQVCRQ LNKPVIVASQ LLESMIEYPT
410 420 430 440 450
PTRAEVADVS EAVRQRADAL MLSGESAMGQ YPEKALAVLR SVSVRIEKWW
460 470 480 490 500
REEKHHEAME LPAIGSTYSD SISEEICNSA AKMANNLGVD ALFVYTKDGH
510 520 530 540 550
MASLLSRCRP DCPIFAFTTT TSVRRRLNLQ WGLIPFRLSF ADDMESNLNK
560 570 580
TFSLLKARGM IKSGDLVIAV SDMLQSIQVM NVP
Length:583
Mass (Da):64,094
Last modified:November 1, 1996 - v1
Checksum:i5DFDE4F778A753C2
GO
Isoform Beta (identifier: Q43117-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-129: MSQSLHFSPN...EELEALAVGG → MAVVVKDLEE...VTAVMGVVGD

Show »
Length:493
Mass (Da):54,462
Checksum:i375858858BF6124F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 129129MSQSL…LAVGG → MAVVVKDLEEAVRVVVLAVL RDMEVVVVLVTAVMGVVGD in isoform Beta. 1 PublicationVSP_002886Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64736 mRNA. Translation: AAA33870.1.
M64737 mRNA. Translation: AAA33871.1.
PIRiT10051.
T10054.
RefSeqiXP_002517044.1. XM_002516998.1. [Q43117-1]

Genome annotation databases

GeneIDi8280200.
KEGGircu:RCOM_0910580.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64736 mRNA. Translation: AAA33870.1 .
M64737 mRNA. Translation: AAA33871.1 .
PIRi T10051.
T10054.
RefSeqi XP_002517044.1. XM_002516998.1. [Q43117-1 ]

3D structure databases

ProteinModelPortali Q43117.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q43117.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 8280200.
KEGGi rcu:RCOM_0910580.

Phylogenomic databases

KOi K00873.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
SABIO-RK Q43117.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Relationship between the subunits of leucoplast pyruvate kinase from Ricinus communis and a comparison with the enzyme from other sources."
    Blakeley S.D., Plaxton W.C., Dennis D.T.
    Plant Physiol. 96:1283-1288(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA), PROTEIN SEQUENCE OF 75-81.
    Strain: cv. Baker 296.
    Tissue: Endosperm.

Entry informationi

Entry nameiKPYA_RICCO
AccessioniPrimary (citable) accession number: Q43117
Secondary accession number(s): Q43118
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3