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Q43117

- KPYA_RICCO

UniProt

Q43117 - KPYA_RICCO

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Protein
Pyruvate kinase isozyme A, chloroplastic
Gene
N/A
Organism
Ricinus communis (Castor bean)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium By similarity.
Potassium By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei134 – 1341Substrate By similarity
Metal bindingi136 – 1361Potassium By similarity
Metal bindingi168 – 1681Potassium By similarity
Metal bindingi169 – 1691Potassium; via carbonyl oxygen By similarity
Sitei331 – 3311Transition state stabilizer By similarity
Metal bindingi333 – 3331Magnesium Reviewed prediction
Binding sitei356 – 3561Substrate; via amide nitrogen By similarity
Metal bindingi357 – 3571Magnesium By similarity
Binding sitei357 – 3571Substrate; via amide nitrogen By similarity
Binding sitei389 – 3891Substrate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

SABIO-RKQ43117.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase isozyme A, chloroplastic (EC:2.7.1.40)
OrganismiRicinus communis (Castor bean)
Taxonomic identifieri3988 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeAcalyphoideaeAcalypheaeRicinus

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7474Chloroplast1 Publication
Add
BLAST
Chaini75 – 583509Pyruvate kinase isozyme A, chloroplastic
PRO_0000016662Add
BLAST

Proteomic databases

PRIDEiQ43117.

Interactioni

Subunit structurei

Oligomer of alpha and beta subunits By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ43117.

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK00873.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Alpha (identifier: Q43117-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSQSLHFSPN LTFAKQPFPK LPLPFPTSNS RYPVNNYKSL SIKASTSPSS    50
SSDPQVLVAD NGTGNSGVLY NNNNKSVTVS DPSSIEVDAV TETELKENGF 100
RSTRRTKLVC TIGPATCGFE ELEALAVGGM NVARINMCHG TREWHKSVIE 150
RVRRLNEEKG FAVAIMMDTE GSEIHMGDLG GASSAKAEDG EIWTFSVRAY 200
DSPRPERTIN VNYDGFAEDV KVGDELLVDG GMVRFEVIEK IGPDVKCRCT 250
DPGLLLPRAN LTFWRDGSLV RERNAMLPTI SSKDWLDIDF GIAEGVDFIA 300
ISFVKSAEVI NHLKSYIAAR SRDSDIAVIA KIESIDSLKN LEEIIRASDG 350
AMVARGDLGA QIPLEQVPSA QQNIVQVCRQ LNKPVIVASQ LLESMIEYPT 400
PTRAEVADVS EAVRQRADAL MLSGESAMGQ YPEKALAVLR SVSVRIEKWW 450
REEKHHEAME LPAIGSTYSD SISEEICNSA AKMANNLGVD ALFVYTKDGH 500
MASLLSRCRP DCPIFAFTTT TSVRRRLNLQ WGLIPFRLSF ADDMESNLNK 550
TFSLLKARGM IKSGDLVIAV SDMLQSIQVM NVP 583
Length:583
Mass (Da):64,094
Last modified:November 1, 1996 - v1
Checksum:i5DFDE4F778A753C2
GO
Isoform Beta (identifier: Q43117-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-129: MSQSLHFSPN...EELEALAVGG → MAVVVKDLEE...VTAVMGVVGD

Show »
Length:493
Mass (Da):54,462
Checksum:i375858858BF6124F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 129129MSQSL…LAVGG → MAVVVKDLEEAVRVVVLAVL RDMEVVVVLVTAVMGVVGD in isoform Beta.
VSP_002886Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64736 mRNA. Translation: AAA33870.1.
M64737 mRNA. Translation: AAA33871.1.
PIRiT10051.
T10054.
RefSeqiXP_002517044.1. XM_002516998.1. [Q43117-1]

Genome annotation databases

GeneIDi8280200.
KEGGircu:RCOM_0910580.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64736 mRNA. Translation: AAA33870.1 .
M64737 mRNA. Translation: AAA33871.1 .
PIRi T10051.
T10054.
RefSeqi XP_002517044.1. XM_002516998.1. [Q43117-1 ]

3D structure databases

ProteinModelPortali Q43117.
ModBasei Search...

Proteomic databases

PRIDEi Q43117.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 8280200.
KEGGi rcu:RCOM_0910580.

Phylogenomic databases

KOi K00873.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
SABIO-RK Q43117.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Relationship between the subunits of leucoplast pyruvate kinase from Ricinus communis and a comparison with the enzyme from other sources."
    Blakeley S.D., Plaxton W.C., Dennis D.T.
    Plant Physiol. 96:1283-1288(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA), PROTEIN SEQUENCE OF 75-81.
    Strain: cv. Baker 296.
    Tissue: Endosperm.

Entry informationi

Entry nameiKPYA_RICCO
AccessioniPrimary (citable) accession number: Q43117
Secondary accession number(s): Q43118
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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