Reviewed, UniProtKB/Swiss-Prot Q43117 (KPYA_RICCO)

Names and origin

Protein names	Recommended name:     Pyruvate kinase isozyme A, chloroplastic     EC=2.7.1.40
Organism	Ricinus communis (Castor bean)
Taxonomic identifier	3988 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Euphorbiaceae › Acalyphoideae › Acalypheae › Ricinus

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Oligomer of alpha and beta subunits By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Alpha (identifier: Q43117-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: Q43117-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-129: MSQSLHFSPN...EELEALAVGG → MAVVVKDLEE...VTAVMGVVGD

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 74	74	Chloroplast Ref.1
	Chain	75 – 583	509	Pyruvate kinase isozyme A, chloroplastic		PRO_0000016662
Sites
	Active site	331	1	By similarity
	Metal binding	333	1	Magnesium Potential
	Metal binding	354	1	Magnesium Potential
	Metal binding	355	1	Magnesium Potential
Natural variations
	Alternative sequence	1 – 129	129	MSQSL…LAVGG → MAVVVKDLEEAVRVVVLAVL RDMEVVVVLVTAVMGVVGD in isoform Beta.		VSP_002886

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5DFDE4F778A753C2	FASTA	583	64,094
	10 20 30 40 50 60 MSQSLHFSPN LTFAKQPFPK LPLPFPTSNS RYPVNNYKSL SIKASTSPSS SSDPQVLVAD 70 80 90 100 110 120 NGTGNSGVLY NNNNKSVTVS DPSSIEVDAV TETELKENGF RSTRRTKLVC TIGPATCGFE 130 140 150 160 170 180 ELEALAVGGM NVARINMCHG TREWHKSVIE RVRRLNEEKG FAVAIMMDTE GSEIHMGDLG 190 200 210 220 230 240 GASSAKAEDG EIWTFSVRAY DSPRPERTIN VNYDGFAEDV KVGDELLVDG GMVRFEVIEK 250 260 270 280 290 300 IGPDVKCRCT DPGLLLPRAN LTFWRDGSLV RERNAMLPTI SSKDWLDIDF GIAEGVDFIA 310 320 330 340 350 360 ISFVKSAEVI NHLKSYIAAR SRDSDIAVIA KIESIDSLKN LEEIIRASDG AMVARGDLGA 370 380 390 400 410 420 QIPLEQVPSA QQNIVQVCRQ LNKPVIVASQ LLESMIEYPT PTRAEVADVS EAVRQRADAL 430 440 450 460 470 480 MLSGESAMGQ YPEKALAVLR SVSVRIEKWW REEKHHEAME LPAIGSTYSD SISEEICNSA 490 500 510 520 530 540 AKMANNLGVD ALFVYTKDGH MASLLSRCRP DCPIFAFTTT TSVRRRLNLQ WGLIPFRLSF 550 560 570 580 ADDMESNLNK TFSLLKARGM IKSGDLVIAV SDMLQSIQVM NVP
	Isoform Beta. Checksum: 375858858BF6124F	FASTA	493	54,462
	10 20 30 40 50 60 MAVVVKDLEE AVRVVVLAVL RDMEVVVVLV TAVMGVVGDM NVARINMCHG TREWHKSVIE 70 80 90 100 110 120 RVRRLNEEKG FAVAIMMDTE GSEIHMGDLG GASSAKAEDG EIWTFSVRAY DSPRPERTIN 130 140 150 160 170 180 VNYDGFAEDV KVGDELLVDG GMVRFEVIEK IGPDVKCRCT DPGLLLPRAN LTFWRDGSLV 190 200 210 220 230 240 RERNAMLPTI SSKDWLDIDF GIAEGVDFIA ISFVKSAEVI NHLKSYIAAR SRDSDIAVIA 250 260 270 280 290 300 KIESIDSLKN LEEIIRASDG AMVARGDLGA QIPLEQVPSA QQNIVQVCRQ LNKPVIVASQ 310 320 330 340 350 360 LLESMIEYPT PTRAEVADVS EAVRQRADAL MLSGESAMGQ YPEKALAVLR SVSVRIEKWW 370 380 390 400 410 420 REEKHHEAME LPAIGSTYSD SISEEICNSA AKMANNLGVD ALFVYTKDGH MASLLSRCRP 430 440 450 460 470 480 DCPIFAFTTT TSVRRRLNLQ WGLIPFRLSF ADDMESNLNK TFSLLKARGM IKSGDLVIAV 490 SDMLQSIQVM NVP

References

[1]

"Relationship between the subunits of leucoplast pyruvate kinase from Ricinus communis and a comparison with the enzyme from other sources." Blakeley S.D., Plaxton W.C., Dennis D.T. Plant Physiol. 96:1283-1288(1991) [PubMed: 16668331] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA), PROTEIN SEQUENCE OF 75-81. Strain: cv. Baker 296. Tissue: Endosperm.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M64736 mRNA. Translation: AAA33870.1. M64737 mRNA. Translation: AAA33871.1.
PIR	T10051. T10054.
3D structure databases
HSSP	HSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.40. 816.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYA_RICCO

Accession

Primary (citable) accession number: Q43117
Secondary accession number(s): Q43118

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)