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Protein

Pectinesterase 3

Gene

MPE3

Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May have roles in the deposition of pectin in developing tissues and in the wall loosening and cell separation that occurs in cell expansion, fruit ripening and abscission.

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase, Pectinesterase (PHAVU_001G217400g), Pectinesterase (PHAVU_003G015600g), Pectinesterase (PHAVU_010G080300g), Pectinesterase (PHAVU_005G104100g), Pectinesterase (PHAVU_009G2224000g), Pectinesterase (PHAVU_009G222500g), Pectinesterase (PHAVU_008G271600g), Pectinesterase (PHAVU_003G127700g), Pectinesterase (PHAVU_007G198100g), Uncharacterized protein (PHAVU_010G123100g), Pectinesterase (PHAVU_001G209200g), Pectinesterase (PHAVU_009G222300g), Pectinesterase (PHAVU_002G048500g), Pectinesterase (PHAVU_009G2224000g), Pectinesterase (PHAVU_009G053000g), Pectinesterase (PHAVU_005G015000g), Pectinesterase (PHAVU_009G222100g), Pectinesterase (PHAVU_002G270900g), Pectinesterase (PHAVU_007G218500g), Pectinesterase (PHAVU_009G222600g), Pectinesterase (PHAVU_007G246700g), Pectinesterase (PHAVU_008G271800g), Pectinesterase (PHAVU_001G209400g), Pectinesterase (PHAVU_005G007800g), Pectinesterase (PHAVU_006G088000g), Pectinesterase (PHAVU_008G158900g), Pectinesterase (PHAVU_008G272000g), Pectinesterase (PHAVU_005G103500g), Pectinesterase (PHAVU_008G271500g), Pectinesterase (PHAVU_006G088100g), Pectinesterase (PHAVU_007G202000g), Pectinesterase (PHAVU_009G148900g), Pectinesterase (PHAVU_010G079900g), Pectinesterase (PHAVU_007G210300g), Pectinesterase (PHAVU_002G048700g), Pectinesterase (PHAVU_007G210200g), Pectinesterase (PHAVU_009G222700g), Pectinesterase (PHAVU_007G210400g), Pectinesterase (PHAVU_001G140600g), Pectinesterase (PHAVU_006G088200g), Pectinesterase (PHAVU_003G126500g), Pectinesterase (PHAVU_004G175200g), Pectinesterase (PHAVU_003G126600g), Pectinesterase (PHAVU_007G198100g), Pectinesterase (PHAVU_003G027000g), Pectinesterase (PHAVU_008G277900g), Pectinesterase (PHAVU_008G039000g), Pectinesterase (PHAVU_002G048600g), Pectinesterase (PHAVU_002G318500g), Pectinesterase (PHAVU_007G201900g), Pectinesterase (PHAVU_003G107300g), Pectinesterase, Pectinesterase 3 (MPE3), Pectinesterase, Pectinesterase, Pectinesterase (PHAVU_007G198200g), Pectinesterase (PHAVU_001G209100g), Pectinesterase (PHAVU_002G048400g), Pectinesterase (PHAVU_003G015700g), Pectinesterase (PHAVU_005G184400g), Pectinesterase (PHAVU_005G011900g), Pectinesterase (PHAVU_005G012000g), Pectinesterase (PHAVU_002G308000g), Pectinesterase (PHAVU_011G1890000g), Pectinesterase (PHAVU_011G005000g), Pectinesterase (PHAVU_002G063100g), Pectinesterase (PHAVU_011G188900g), Pectinesterase (PHAVU_008G278000g), Pectinesterase (PHAVU_003G220500g), Pectinesterase (PHAVU_001G209300g), Pectinesterase (PHAVU_009G250600g), Pectinesterase (PHAVU_001G183100g), Pectinesterase (PHAVU_005G011900g), Pectinesterase (PHAVU_007G212000g), Pectinesterase (PHAVU_009G261700g), Pectinesterase (PHAVU_003G126700g), Pectinesterase (PHAVU_001G217300g), Pectinesterase (PHAVU_010G080000g)
  2. Pectate lyase (PHAVU_003G207400g), Pectate lyase (PHAVU_006G147500g), Pectate lyase (PHAVU_009G189800g), Pectate lyase (PHAVU_004G026400g), Pectate lyase (PHAVU_008G123000g), Pectate lyase (PHAVU_001G256900g), Pectate lyase (PHAVU_009G125800g), Pectate lyase (PHAVU_001G256800g), Pectate lyase (PHAVU_004G101600g), Pectate lyase (PHAVU_009G170500g), Pectate lyase (PHAVU_007G009300g), Pectate lyase (PHAVU_007G024400g), Pectate lyase (PHAVU_004G098100g), Pectate lyase (PHAVU_008G043600g), Pectate lyase (PHAVU_007G160100g), Pectate lyase (PHAVU_004G059200g), Pectate lyase (PHAVU_007G151200g), Pectate lyase (PHAVU_007G236800g), Pectate lyase (PHAVU_002G065800g), Pectate lyase (PHAVU_002G025800g), Pectate lyase (PHAVU_001G180800g), Pectate lyase (PHAVU_006G147600g), Pectate lyase (PHAVU_004G101900g), Pectate lyase (PHAVU_003G081000g), Pectate lyase (PHAVU_003G069100g)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei348SubstrateBy similarity1
Binding sitei378SubstrateBy similarity1
Sitei400Transition state stabilizerBy similarity1
Active sitei401Proton donorPROSITE-ProRule annotation1
Active sitei422NucleophilePROSITE-ProRule annotation1
Binding sitei486SubstrateBy similarity1
Binding sitei488SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectinesterase 3 (EC:3.1.1.11)
Short name:
PE 3
Alternative name(s):
Pectin methylesterase 3
Gene namesi
Name:MPE3
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 55Sequence analysisAdd BLAST55
ChainiPRO_000002349656 – 581Pectinesterase 3Add BLAST526

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi101N-linked (GlcNAc...)Sequence analysis1
Glycosylationi156N-linked (GlcNAc...)Sequence analysis1
Glycosylationi200N-linked (GlcNAc...)Sequence analysis1
Glycosylationi217N-linked (GlcNAc...)Sequence analysis1
Glycosylationi268N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi415 ↔ 435By similarity
Glycosylationi477N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ43111.

Structurei

3D structure databases

ProteinModelPortaliQ43111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTIKSFKGY GKVNELEQQA YEKKTRKRLI IIAVSSIVLI AVIIAAVAGV
60 70 80 90 100
VIHNRNSESS PSSDSVPQTE LSPAASLKAV CDTTRYPSSC FSSISSLPES
110 120 130 140 150
NTTDPELLFK LSLRVAIDEL SSFPSKLRAN AEQDARLQKA IDVCSSVFGD
160 170 180 190 200
ALDRLNDSIS ALGTVAGRIA SSASVSNVET WLSAALTDQD TCLDAVGELN
210 220 230 240 250
STAARGALQE IETAMRNSTE FASNSLAIVT KILGLLSRFE TPIHHRRLLG
260 270 280 290 300
FPEWLGAAER RLLEEKNNDS TPDAVVAKDG SGQFKTIGEA LKLVKKKSEE
310 320 330 340 350
RFSVYVKEGR YVENIDLDKN TWNVMIYGDG KDKTFVVGSR NFMDGTPTFE
360 370 380 390 400
TATFAVKGKG FIAKDIGFVN NAGASKHQAV ALRSGSDRSV FFRCSFDGFQ
410 420 430 440 450
DTLYAHSNRQ FYRDCDITGT IDFIFGNAAV VFQSCKIMPR QPLPNQFNTI
460 470 480 490 500
TAQGKKDPNQ NTGIIIQKST ITPFGNNLTA PTYLGRPWKD FSTTVIMQSD
510 520 530 540 550
IGALLNPVGW MSWVPNVEPP TTIFYAEYQN SGPGADVSQR VKWAGYKPTI
560 570 580
TDRNAEEFTV QSFIQGPEWL PNAAVQFDST L
Length:581
Mass (Da):63,588
Last modified:November 1, 1996 - v1
Checksum:iCDB0621372E59BFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85216 mRNA. Translation: CAA59482.1.
PIRiS53105.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85216 mRNA. Translation: CAA59482.1.
PIRiS53105.

3D structure databases

ProteinModelPortaliQ43111.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ43111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPME3_PHAVU
AccessioniPrimary (citable) accession number: Q43111
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.