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Protein

Pectinesterase 3

Gene

MPE3

Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May have roles in the deposition of pectin in developing tissues and in the wall loosening and cell separation that occurs in cell expansion, fruit ripening and abscission.

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase (PHAVU_008G277900g), Pectinesterase, Pectinesterase (PHAVU_001G217400g), Pectinesterase (PHAVU_003G015600g), Pectinesterase (PHAVU_010G080300g), Pectinesterase (PHAVU_008G039000g), Pectinesterase (PHAVU_005G104100g), Pectinesterase (PHAVU_009G2224000g), Pectinesterase (PHAVU_002G048600g), Pectinesterase (PHAVU_009G222500g), Pectinesterase (PHAVU_007G201900g), Pectinesterase (PHAVU_002G318500g), Pectinesterase (PHAVU_003G107300g), Pectinesterase (PHAVU_007G198100g), Pectinesterase, Uncharacterized protein (PHAVU_010G123100g), Pectinesterase 3 (MPE3), Pectinesterase, Pectinesterase (PHAVU_001G209200g), Pectinesterase (PHAVU_002G048500g), Pectinesterase (PHAVU_009G222300g), Pectinesterase (PHAVU_009G2224000g), Pectinesterase (PHAVU_002G048400g), Pectinesterase (PHAVU_001G209100g), Pectinesterase (PHAVU_007G198200g), Pectinesterase (PHAVU_009G053000g), Pectinesterase (PHAVU_005G015000g), Pectinesterase (PHAVU_009G222100g), Uncharacterized protein (PHAVU_002G270900g), Pectinesterase (PHAVU_007G218500g), Pectinesterase (PHAVU_009G222600g), Pectinesterase (PHAVU_007G246700g), Pectinesterase (PHAVU_005G011900g), Pectinesterase (PHAVU_005G012000g), Pectinesterase (PHAVU_001G209400g), Pectinesterase (PHAVU_005G007800g), Pectinesterase (PHAVU_011G1890000g), Pectinesterase (PHAVU_002G308000g), Pectinesterase (PHAVU_011G005000g), Pectinesterase (PHAVU_002G063100g), Pectinesterase (PHAVU_006G088000g), Pectinesterase (PHAVU_011G188900g), Pectinesterase (PHAVU_003G220500g), Pectinesterase (PHAVU_008G158900g), Pectinesterase (PHAVU_001G209300g), Uncharacterized protein (PHAVU_005G103500g), Pectinesterase (PHAVU_007G202000g), Pectinesterase (PHAVU_006G088100g), Pectinesterase (PHAVU_009G250600g), Pectinesterase (PHAVU_001G183100g), Pectinesterase (PHAVU_005G011900g), Uncharacterized protein (PHAVU_009G148900g), Pectinesterase (PHAVU_010G079900g), Pectinesterase (PHAVU_007G212000g), Pectinesterase (PHAVU_009G261700g), Pectinesterase (PHAVU_007G210300g), Pectinesterase (PHAVU_002G048700g), Pectinesterase (PHAVU_007G210200g), Pectinesterase (PHAVU_009G222700g), Pectinesterase (PHAVU_007G210400g), Pectinesterase (PHAVU_001G140600g), Pectinesterase (PHAVU_006G088200g), Pectinesterase (PHAVU_003G126500g), Pectinesterase (PHAVU_003G126700g), Pectinesterase (PHAVU_004G175200g), Pectinesterase (PHAVU_003G126600g), Pectinesterase (PHAVU_010G080000g), Pectinesterase (PHAVU_001G217300g), Pectinesterase (PHAVU_007G198100g)
  2. Pectate lyase (PHAVU_003G207400g), Pectate lyase (PHAVU_007G024400g), Pectate lyase (PHAVU_006G147500g), Pectate lyase (PHAVU_004G098100g), Pectate lyase (PHAVU_009G189800g), Pectate lyase (PHAVU_008G043600g), Pectate lyase (PHAVU_007G160100g), Pectate lyase (PHAVU_004G059200g), Pectate lyase (PHAVU_007G151200g), Pectate lyase (PHAVU_004G026400g), Pectate lyase (PHAVU_008G123000g), Pectate lyase (PHAVU_001G256900g), Pectate lyase (PHAVU_007G236800g), Pectate lyase (PHAVU_009G125800g), Pectate lyase (PHAVU_001G256800g), Pectate lyase (PHAVU_002G065800g), Pectate lyase (PHAVU_002G025800g), Pectate lyase (PHAVU_001G180800g), Pectate lyase (PHAVU_004G101900g), Pectate lyase (PHAVU_006G147600g), Pectate lyase (PHAVU_004G101600g), Pectate lyase (PHAVU_003G069100g), Pectate lyase (PHAVU_003G081000g), Pectate lyase (PHAVU_009G170500g), Pectate lyase (PHAVU_007G009300g)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei348SubstrateBy similarity1
Binding sitei378SubstrateBy similarity1
Sitei400Transition state stabilizerBy similarity1
Active sitei401Proton donorPROSITE-ProRule annotation1
Active sitei422NucleophilePROSITE-ProRule annotation1
Binding sitei486SubstrateBy similarity1
Binding sitei488SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl esterase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823

Names & Taxonomyi

Protein namesi
Recommended name:
Pectinesterase 3 (EC:3.1.1.11)
Short name:
PE 3
Alternative name(s):
Pectin methylesterase 3
Gene namesi
Name:MPE3
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 55Sequence analysisAdd BLAST55
ChainiPRO_000002349656 – 581Pectinesterase 3Add BLAST526

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi101N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi156N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi200N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi217N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi268N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi415 ↔ 435By similarity
Glycosylationi477N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ43111
SMRiQ43111
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
IPR018040 Pectinesterase_Tyr_AS
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
TIGRFAMsiTIGR01614 PME_inhib, 1 hit
PROSITEiView protein in PROSITE
PS00800 PECTINESTERASE_1, 1 hit
PS00503 PECTINESTERASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTIKSFKGY GKVNELEQQA YEKKTRKRLI IIAVSSIVLI AVIIAAVAGV
60 70 80 90 100
VIHNRNSESS PSSDSVPQTE LSPAASLKAV CDTTRYPSSC FSSISSLPES
110 120 130 140 150
NTTDPELLFK LSLRVAIDEL SSFPSKLRAN AEQDARLQKA IDVCSSVFGD
160 170 180 190 200
ALDRLNDSIS ALGTVAGRIA SSASVSNVET WLSAALTDQD TCLDAVGELN
210 220 230 240 250
STAARGALQE IETAMRNSTE FASNSLAIVT KILGLLSRFE TPIHHRRLLG
260 270 280 290 300
FPEWLGAAER RLLEEKNNDS TPDAVVAKDG SGQFKTIGEA LKLVKKKSEE
310 320 330 340 350
RFSVYVKEGR YVENIDLDKN TWNVMIYGDG KDKTFVVGSR NFMDGTPTFE
360 370 380 390 400
TATFAVKGKG FIAKDIGFVN NAGASKHQAV ALRSGSDRSV FFRCSFDGFQ
410 420 430 440 450
DTLYAHSNRQ FYRDCDITGT IDFIFGNAAV VFQSCKIMPR QPLPNQFNTI
460 470 480 490 500
TAQGKKDPNQ NTGIIIQKST ITPFGNNLTA PTYLGRPWKD FSTTVIMQSD
510 520 530 540 550
IGALLNPVGW MSWVPNVEPP TTIFYAEYQN SGPGADVSQR VKWAGYKPTI
560 570 580
TDRNAEEFTV QSFIQGPEWL PNAAVQFDST L
Length:581
Mass (Da):63,588
Last modified:November 1, 1996 - v1
Checksum:iCDB0621372E59BFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85216 mRNA Translation: CAA59482.1
PIRiS53105

Genome annotation databases

EnsemblPlantsiESW07357; ESW07357; PHAVU_010G123100g
GrameneiESW07357; ESW07357; PHAVU_010G123100g

Similar proteinsi

Entry informationi

Entry nameiPME3_PHAVU
AccessioniPrimary (citable) accession number: Q43111
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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