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Protein

Pectinesterase 3

Gene

MPE3

Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May have roles in the deposition of pectin in developing tissues and in the wall loosening and cell separation that occurs in cell expansion, fruit ripening and abscission.

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase (PHAVU_001G217400g), Pectinesterase, Pectinesterase (PHAVU_003G015600g), Pectinesterase (PHAVU_010G080300g), Pectinesterase (PHAVU_005G104100g), Pectinesterase (PHAVU_009G2224000g), Pectinesterase (PHAVU_009G222500g), Pectinesterase (PHAVU_008G271600g), Pectinesterase (PHAVU_003G127700g), Pectinesterase (PHAVU_007G198100g), Uncharacterized protein (PHAVU_010G123100g), Pectinesterase (PHAVU_001G209200g), Pectinesterase (PHAVU_002G048500g), Pectinesterase (PHAVU_009G222300g), Pectinesterase (PHAVU_009G2224000g), Pectinesterase (PHAVU_009G053000g), Pectinesterase (PHAVU_009G222100g), Pectinesterase (PHAVU_005G015000g), Pectinesterase (PHAVU_002G270900g), Pectinesterase (PHAVU_007G218500g), Pectinesterase (PHAVU_009G222600g), Pectinesterase (PHAVU_007G246700g), Pectinesterase (PHAVU_008G271800g), Pectinesterase (PHAVU_001G209400g), Pectinesterase (PHAVU_005G007800g), Pectinesterase (PHAVU_006G088000g), Pectinesterase (PHAVU_008G158900g), Pectinesterase (PHAVU_008G272000g), Pectinesterase (PHAVU_005G103500g), Pectinesterase (PHAVU_008G271500g), Pectinesterase (PHAVU_006G088100g), Pectinesterase (PHAVU_007G202000g), Pectinesterase (PHAVU_009G148900g), Pectinesterase (PHAVU_010G079900g), Pectinesterase (PHAVU_007G210300g), Pectinesterase (PHAVU_002G048700g), Pectinesterase (PHAVU_007G210200g), Pectinesterase (PHAVU_009G222700g), Pectinesterase (PHAVU_007G210400g), Pectinesterase (PHAVU_001G140600g), Pectinesterase (PHAVU_006G088200g), Pectinesterase (PHAVU_003G126500g), Pectinesterase (PHAVU_004G175200g), Pectinesterase (PHAVU_003G126600g), Pectinesterase (PHAVU_007G198100g), Pectinesterase (PHAVU_003G027000g), Pectinesterase (PHAVU_008G277900g), Pectinesterase (PHAVU_008G039000g), Pectinesterase (PHAVU_002G048600g), Pectinesterase (PHAVU_002G318500g), Pectinesterase (PHAVU_007G201900g), Pectinesterase (PHAVU_003G107300g), Pectinesterase, Pectinesterase 3 (MPE3), Pectinesterase, Pectinesterase, Pectinesterase (PHAVU_001G209100g), Pectinesterase (PHAVU_002G048400g), Pectinesterase (PHAVU_007G198200g), Pectinesterase (PHAVU_003G015700g), Pectinesterase (PHAVU_005G184400g), Pectinesterase (PHAVU_005G011900g), Pectinesterase (PHAVU_005G012000g), Pectinesterase (PHAVU_011G005000g), Pectinesterase (PHAVU_011G1890000g), Pectinesterase (PHAVU_002G308000g), Pectinesterase (PHAVU_002G063100g), Pectinesterase (PHAVU_011G188900g), Pectinesterase (PHAVU_008G278000g), Pectinesterase (PHAVU_003G220500g), Pectinesterase (PHAVU_001G209300g), Pectinesterase (PHAVU_009G250600g), Pectinesterase (PHAVU_001G183100g), Pectinesterase (PHAVU_005G011900g), Pectinesterase (PHAVU_007G212000g), Pectinesterase (PHAVU_009G261700g), Pectinesterase (PHAVU_003G126700g), Pectinesterase (PHAVU_001G217300g), Pectinesterase (PHAVU_010G080000g)
  2. Pectate lyase (PHAVU_003G207400g), Pectate lyase (PHAVU_006G147500g), Pectate lyase (PHAVU_009G189800g), Pectate lyase (PHAVU_004G026400g), Pectate lyase (PHAVU_008G123000g), Pectate lyase (PHAVU_001G256900g), Pectate lyase (PHAVU_009G125800g), Pectate lyase (PHAVU_001G256800g), Pectate lyase (PHAVU_004G101600g), Pectate lyase (PHAVU_009G170500g), Pectate lyase (PHAVU_007G009300g), Pectate lyase (PHAVU_007G024400g), Pectate lyase (PHAVU_004G098100g), Pectate lyase (PHAVU_008G043600g), Pectate lyase (PHAVU_007G160100g), Pectate lyase (PHAVU_004G059200g), Pectate lyase (PHAVU_007G151200g), Pectate lyase (PHAVU_007G236800g), Pectate lyase (PHAVU_002G065800g), Pectate lyase (PHAVU_002G025800g), Pectate lyase (PHAVU_001G180800g), Pectate lyase (PHAVU_004G101900g), Pectate lyase (PHAVU_006G147600g), Pectate lyase (PHAVU_003G069100g), Pectate lyase (PHAVU_003G081000g)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei348 – 3481SubstrateBy similarity
Binding sitei378 – 3781SubstrateBy similarity
Sitei400 – 4001Transition state stabilizerBy similarity
Active sitei401 – 4011Proton donorPROSITE-ProRule annotation
Active sitei422 – 4221NucleophilePROSITE-ProRule annotation
Binding sitei486 – 4861SubstrateBy similarity
Binding sitei488 – 4881SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectinesterase 3 (EC:3.1.1.11)
Short name:
PE 3
Alternative name(s):
Pectin methylesterase 3
Gene namesi
Name:MPE3
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5555Sequence analysisAdd
BLAST
Chaini56 – 581526Pectinesterase 3PRO_0000023496Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence analysis
Glycosylationi156 – 1561N-linked (GlcNAc...)Sequence analysis
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence analysis
Glycosylationi217 – 2171N-linked (GlcNAc...)Sequence analysis
Glycosylationi268 – 2681N-linked (GlcNAc...)Sequence analysis
Disulfide bondi415 ↔ 435By similarity
Glycosylationi477 – 4771N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ43111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTIKSFKGY GKVNELEQQA YEKKTRKRLI IIAVSSIVLI AVIIAAVAGV
60 70 80 90 100
VIHNRNSESS PSSDSVPQTE LSPAASLKAV CDTTRYPSSC FSSISSLPES
110 120 130 140 150
NTTDPELLFK LSLRVAIDEL SSFPSKLRAN AEQDARLQKA IDVCSSVFGD
160 170 180 190 200
ALDRLNDSIS ALGTVAGRIA SSASVSNVET WLSAALTDQD TCLDAVGELN
210 220 230 240 250
STAARGALQE IETAMRNSTE FASNSLAIVT KILGLLSRFE TPIHHRRLLG
260 270 280 290 300
FPEWLGAAER RLLEEKNNDS TPDAVVAKDG SGQFKTIGEA LKLVKKKSEE
310 320 330 340 350
RFSVYVKEGR YVENIDLDKN TWNVMIYGDG KDKTFVVGSR NFMDGTPTFE
360 370 380 390 400
TATFAVKGKG FIAKDIGFVN NAGASKHQAV ALRSGSDRSV FFRCSFDGFQ
410 420 430 440 450
DTLYAHSNRQ FYRDCDITGT IDFIFGNAAV VFQSCKIMPR QPLPNQFNTI
460 470 480 490 500
TAQGKKDPNQ NTGIIIQKST ITPFGNNLTA PTYLGRPWKD FSTTVIMQSD
510 520 530 540 550
IGALLNPVGW MSWVPNVEPP TTIFYAEYQN SGPGADVSQR VKWAGYKPTI
560 570 580
TDRNAEEFTV QSFIQGPEWL PNAAVQFDST L
Length:581
Mass (Da):63,588
Last modified:November 1, 1996 - v1
Checksum:iCDB0621372E59BFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85216 mRNA. Translation: CAA59482.1.
PIRiS53105.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85216 mRNA. Translation: CAA59482.1.
PIRiS53105.

3D structure databases

ProteinModelPortaliQ43111.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of pectinases and pectin methylesterase cDNAs in pods of green beans (Phaseolus vulgaris L.)."
    Ebbelaar C.E.M., Tucker G.A., Laats J.M., van Dijk C., Stolle-Smits T., Recourt K.
    Plant Mol. Biol. 31:1141-1151(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Masai.

Entry informationi

Entry nameiPME3_PHAVU
AccessioniPrimary (citable) accession number: Q43111
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: March 16, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.