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Protein

Isocitrate lyase

Gene

ICL 8

Organism
Pinus taeda (Loblolly pine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in storage lipid mobilization during the growth of higher plant seedling.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (ICL 8)
  2. no protein annotated in this organism
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi177MagnesiumBy similarity1
Active sitei215Proton acceptorBy similarity1
Binding sitei252SubstrateBy similarity1
Binding sitei476SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLBy similarity
Alternative name(s):
IsocitraseBy similarity
IsocitratsysaseBy similarity
Gene namesi
Name:ICL 8
OrganismiPinus taeda (Loblolly pine)
Taxonomic identifieri3352 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaePinusPinus

Subcellular locationi

  • Glyoxysome By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688091 – 580Isocitrate lyaseAdd BLAST580

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ43097.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni106 – 108Substrate bindingBy similarity3
Regioni216 – 217Substrate bindingBy similarity2
Regioni441 – 445Substrate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi578 – 580Microbody targeting signalSequence analysis3

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q43097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIYSAQAPN SILEEEARFE AEVSETQAWW NSTDLFRLTR RPYTARDVVR
60 70 80 90 100
LRGSMRQSYA SNEMAKKLWR TLKTHQANKT ASRTFGALDP VQVSMMAKYL
110 120 130 140 150
DSIYVSGWQC SSTHTTTNEP GPDLADYPYD TVPNKVEHLF FAQQFHDRKQ
160 170 180 190 200
KEARMSMTRE ERSKTPYIDY LKPIIADGDT GFGGATATVK LCKLFVERGA
210 220 230 240 250
AGVHIEDQAS VTKKCGHMAG KVLVSVGEHV NRMVAARLQF DIMGVETLLV
260 270 280 290 300
ARTDAVAATL IQTNVDARDH QFILGATNPN LKGKPLADVL ARAMASGKSG
310 320 330 340 350
ADLQAVEDEW MAMADLKLFS DCVVDGIKAL NVSEQEKGRR LGEWMQQTGG
360 370 380 390 400
NTGNVLSYYQ AKELAEKLGI SNLFWDWDLP RTREGFYRFQ GSVKAAIVRG
410 420 430 440 450
WAFGPHADII WMETSSPDMV ECRDFALGVK SKHPEIMLAY NLSPSFNWDA
460 470 480 490 500
SRMTDEQMKN FIPEIARLGY CWQFITLAGF HADALVIDTF AKDFAQRGML
510 520 530 540 550
AYVEKIQRQE MMNGVDTLAH QKWSGANYYD QLLKTVQGGI SATAAMAKGV
560 570 580
TEDQFEETQS STLALESNIG AGTVLAKSRM
Length:580
Mass (Da):64,676
Last modified:November 1, 1996 - v1
Checksum:iE85785DAE970D697
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39807 mRNA. Translation: AAC49687.1.
PIRiT09779.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39807 mRNA. Translation: AAC49687.1.
PIRiT09779.

3D structure databases

ProteinModelPortaliQ43097.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACEA_PINTA
AccessioniPrimary (citable) accession number: Q43097
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.