Q43095 (CAMT_POPTM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Caffeoyl-CoA O-methyltransferase EC=2.1.1.104 Alternative name(s): Trans-caffeoyl-CoA 3-O-methyltransferase Short name=CCoAMT Short name=CCoAOMT
Organism	Populus tremuloides (Quaking aspen)
Taxonomic identifier	3693 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Malpighiales › Salicaceae › Saliceae › Populus

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Methylates caffeoyl-CoA to feruloyl-CoA and 5-hydroxyferuloyl-CoA to sinapoyl-CoA. Plays a role in the synthesis of feruloylated polysaccharides. Involved in the reinforcement of the plant cell wall. Also involved in the responding to wounding or pathogen challenge by the increased formation of cell wall-bound ferulic acid polymers.
Catalytic activity	S-adenosyl-L-methionine + caffeoyl-CoA = S-adenosyl-L-homocysteine + feruloyl-CoA.
Cofactor	Binds 1 divalent metal cation per subunit By similarity.
Pathway	Aromatic compound metabolism; phenylpropanoid biosynthesis.
Sequence similarities	Belongs to the methyltransferase superfamily. Type 3 family. CCoAMT subfamily.

Ontologies

Keywords
Biological process	Lignin biosynthesis
Ligand	Metal-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	lignin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	caffeoyl-CoA O-methyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	Caffeoyl-CoA O-methyltransferase		PRO_0000165692
Regions
	Region	87 – 88	2	S-adenosyl-L-methionine binding By similarity
Sites
	Metal binding	163	1	Divalent metal cation By similarity
	Metal binding	189	1	Divalent metal cation By similarity
	Metal binding	190	1	Divalent metal cation By similarity
	Binding site	21	1	Substrate; via amide nitrogen By similarity
	Binding site	63	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	85	1	S-adenosyl-L-methionine By similarity
	Binding site	93	1	S-adenosyl-L-methionine By similarity
	Binding site	111	1	S-adenosyl-L-methionine By similarity
	Binding site	112	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	140	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	163	1	Substrate By similarity
	Binding site	165	1	S-adenosyl-L-methionine By similarity
	Binding site	194	1	Substrate By similarity
	Binding site	206	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43095 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8A9328C7D3021CF1	FASTA	247	27,867
	10 20 30 40 50 60 MATNGEEQQS QAGRHQEVGH KSLLQSDALY QYILETSVYP REPECMKELR EVTAKHPWNI 70 80 90 100 110 120 MTTSADEGQF LNMLLKLVNA KNTMEIGVYT GYSLLATALA IPEDGKILAM DINRENYELG 130 140 150 160 170 180 LPVIQKAGVA HKIDFKEGPA LPVLDQMIED GKYHGSFDFI FVDADKDNYI NYHKRLIELV 190 200 210 220 230 240 KVGGLIGYDN TLWNGSVVAP PDAPMRKYVR YYRDFVLELN KALAADPRIE ICMLPVGDGI TLCRRIQ

References

[1]	"Cloning of Aspen (Populus tremuloides) Xylem caffeoyl-CoA 3-O-methyltransferase." Meng H., Campbell W.H. Plant Gene Register PGR95-040 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Xylem.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U27116 mRNA. Translation: AAA80651.1.
PIR	T09757.
3D structure databases
ProteinModelPortal	Q43095.
SMR	Q43095. Positions 21-247.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00711.
Family and domain databases
InterPro	IPR002935. O-MeTrfase_3. [Graphical view]
PANTHER	PTHR10509. PTHR10509. 1 hit.
Pfam	PF01596. Methyltransf_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAMT_POPTM

Accession

Primary (citable) accession number: Q43095

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	October 3, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program