ID   NIR_PINSY               Reviewed;         105 AA.
AC   Q43090;
DT   10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 53.
DE   RecName: Full=Ferredoxin--nitrite reductase, chloroplastic;
DE            EC=1.7.7.1;
DE   AltName: Full=PSNiR;
DE            Short=NiR;
DE   Flags: Fragment;
GN   Name=NIR;
OS   Pinus sylvestris (Scots pine).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Coniferopsida; Coniferales; Pinaceae; Pinus; Pinus.
OX   NCBI_TaxID=3349;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
RC   STRAIN=cv. Black forest provenance;
RX   MEDLINE=94325469; PubMed=8049370; DOI=10.1007/BF00043873;
RA   Neininger A., Seith B., Hoch B., Mohr H.;
RT   "Gene expression of nitrite reductase in Scots pine (Pinus sylvestris
RT   L.) as affected by light and nitrate.";
RL   Plant Mol. Biol. 25:449-457(1994).
CC   -!- CATALYTIC ACTIVITY: NH(3) + 2 H(2)O + 6 oxidized ferredoxin =
CC       nitrite + 6 reduced ferredoxin + 7 H(+).
CC   -!- COFACTOR: Binds 1 siroheme per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
CC   -!- PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast (By similarity).
CC   -!- TISSUE SPECIFICITY: Highest expression in roots and hypocotyls.
CC       Some expression in cotyledonary whorls.
CC   -!- INDUCTION: Strong induction by nitrate in roots and hypocotyls.
CC       Strong induction by light and weak induction by nitrate in
CC       cotyledonary whorls.
CC   -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S
CC       domain family.
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DR   EMBL; X74949; CAA52905.1; -; mRNA.
DR   PIR; S46311; S46311.
DR   SMR; Q43090; 1-100.
DR   BRENDA; 1.7.7.1; 1355.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0048307; F:ferredoxin-nitrite reductase activity; IEA:EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR006066; Nir_Si_BS.
DR   PROSITE; PS00365; NIR_SIR; 1.
PE   2: Evidence at transcript level;
KW   4Fe-4S; Chloroplast; Heme; Iron; Iron-sulfur; Metal-binding;
KW   Nitrate assimilation; Oxidoreductase; Plastid.
FT   CHAIN        <1    105       Ferredoxin--nitrite reductase,
FT                                chloroplastic.
FT                                /FTId=PRO_0000199955.
FT   METAL        28     28       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL        32     32       Iron (siroheme axial ligand) (By
FT                                similarity).
FT   METAL        32     32       Iron-sulfur (4Fe-4S) (By similarity).
FT   NON_TER       1      1
SQ   SEQUENCE   105 AA;  11597 MW;  1A9E45BF8846017E CRC64;
     KARALKITEE LDRTMEVPKP VRMHWTGCPN TCAQVQVADI GFMGCMTRDE NKKVVEGVDI
     FIGGRVGADS HLGDLIHKGV PCKDVVPVVQ ELLIKHFGAI RKTNM
//