Reviewed, UniProtKB/Swiss-Prot Q43090 (NIR_PINSY)

Names and origin

Protein names

Recommended name:
    Ferredoxin--nitrite reductase, chloroplastic
    EC=1.7.7.1
Alternative name(s):
    PSNiR
      Short name=NiR

Gene names

Name:

NIR

Organism

Pinus sylvestris (Scots pine)

Taxonomic identifier

3349 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Coniferopsida › Coniferales › Pinaceae › Pinus › Pinus

Protein attributes

Sequence length	105 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	NH(3) + 2 H(2)O + 6 oxidized ferredoxin = nitrite + 6 reduced ferredoxin + 7 H(+).
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (assimilation).
Subunit structure	Monomer By similarity.
Subcellular location	Plastid › chloroplastBy similarity.
Tissue specificity	Highest expression in roots and hypocotyls. Some expression in cotyledonary whorls.
Induction	Strong induction by nitrate in roots and hypocotyls. Strong induction by light and weak induction by nitrate in cotyledonary whorls.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Nitrate assimilation
Cellular component	Chloroplast Plastid
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin-nitrite reductase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 105	›105	Ferredoxin--nitrite reductase, chloroplastic		PRO_0000199955
Sites
	Metal binding	28	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	32	1	Iron (siroheme axial ligand) By similarity
	Metal binding	32	1	Iron-sulfur (4Fe-4S) By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q43090-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1A9E45BF8846017E	FASTA	105	11,597
	10 20 30 40 50 60 KARALKITEE LDRTMEVPKP VRMHWTGCPN TCAQVQVADI GFMGCMTRDE NKKVVEGVDI 70 80 90 100 FIGGRVGADS HLGDLIHKGV PCKDVVPVVQ ELLIKHFGAI RKTNM

References

[1]

"Gene expression of nitrite reductase in Scots pine (Pinus sylvestris L.) as affected by light and nitrate." Neininger A., Seith B., Hoch B., Mohr H. Plant Mol. Biol. 25:449-457(1994) [PubMed: 8049370] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Black forest provenance.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X74949 mRNA. Translation: CAA52905.1.
PIR	S46311.
3D structure databases
SMR	Q43090. Positions 1-100.
ModBase	Search...
Family and domain databases
InterPro	IPR006066. Nir_Si_BS. [Graphical view]
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIR_PINSY

Accession

Primary (citable) accession number: Q43090

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2003
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)