Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q43089 (INV1_PEA)

Last modified April 14, 2009. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Beta-fructofuranosidase, cell wall isozyme
    EC=3.2.1.26
Alternative name(s):
    Sucrose hydrolase
    Acid invertase
Gene names
Name: BFRUCT1
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length555 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.

Sequence similarities

Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionbeta-fructofuranosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 555533Beta-fructofuranosidase, cell wall isozyme
PRO_0000033385

Regions

Region58 – 614Substrate binding By similarity
Region120 – 1212Substrate binding By similarity
Region186 – 1872Substrate binding By similarity

Sites

Active site611 By similarity
Active site1401 By similarity
Binding site771Substrate By similarity
Binding site851Substrate By similarity
Binding site2411Substrate By similarity
Binding site2771Substrate By similarity

Amino acid modifications

Glycosylation1541N-linked (GlcNAc...) Potential
Glycosylation1811N-linked (GlcNAc...) Potential
Glycosylation3371N-linked (GlcNAc...) Potential
Disulfide bond435 ↔ 481 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q43089-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7B548E251CFD5D44

FASTA55562,655
        10         20         30         40         50         60 
MAISSIFLLS LFSLIYVIPI EATHHVYQTL ETLSSHHSSK SNHQPYRTAY HFQPLKNWIN 

        70         80         90        100        110        120 
DPNGPMRYGG FYHLFYQYNP KGAVWGNIVW AHSVSKDLVN WTPLDHAIHP SQPSDIKGCW 

       130        140        150        160        170        180 
SGSATILPGG KPAILYTGID PNNHQVQNIA IPKNMSDPLL REWKKSPKNP LMEPTIANKI 

       190        200        210        220        230        240 
NSSSFRDPTT SWLGKDGFWR VLIGSKIDTK GMAILYKSKN FVDWVEAKHP LHSAEGTGMW 

       250        260        270        280        290        300 
ECPDFYPVLD KNLLRTGVDT SRNGDDDVRH VLKVSLDDTK HDHYLIGSYD VVKDVFVPEN 

       310        320        330        340        350        360 
GFEDNGFVLR YDYGKYYASK TFFDDGKNRR ILLGWVNESS SVADDVKKGW SGIHTIPREI 

       370        380        390        400        410        420 
WLHESGKQLV QWPVKEIENL RMNPVNWPTK VIKGGERISI TGVDSVQADV EISFEISDLG 

       430        440        450        460        470        480 
KVESLRKWID PQLLCSQKGA GVKGGVGPFG LLVFASQGLK EYTAVFFRIF KYQDKNLVLM 

       490        500        510        520        530        540 
CSDQSRSSLN KDNDMTSYGT FVDVDPLHEK LSLRTLIDHS VVESFGGEGR ACVTARVYPT 

       550 
LAIHDKAMLK LTSEY 

« Hide

References

[1]"A cDNA clone encoding a cell Wall invertase from pea."
Zhang L., Cohn N.S., Mitchell J.P.
Plant Gene Register PGR96-008
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Little Marvel.

Cross-references

Sequence databases

X85327 mRNA. Translation: CAA59677.1.
PIRT06491.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGH32. Glycoside Hydrolase Family 32.

Enzyme and pathway databases

BRENDA3.2.1.26. 287.

Family and domain databases

InterProIPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
[Graphical view]
PfamPF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTSM00640. Glyco_32. 1 hit.
[Graphical view]
PROSITEPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameINV1_PEA
AccessionPrimary (citable) accession number: Q43089
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 14, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents