Reviewed, UniProtKB/Swiss-Prot Q43067 (TCMO_PEA)

Names and origin

Protein names

Recommended name:
    Trans-cinnamate 4-monooxygenase
    EC=1.14.13.11
Alternative name(s):
    Cinnamic acid 4-hydroxylase
      Short name=CA4H
      Short name=C4H
    Cytochrome P450C4H
    Cytochrome P450 73

Gene names

Name:

CYP73A9

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.
Catalytic activity	Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Phenylpropanoid metabolism; trans-4-coumarate biosynthesis; trans-4-coumarate from trans-cinnamate: step 1/1.
Induction	By wounding.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro trans-cinnamate 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Trans-cinnamate 4-monooxygenase		PRO_0000052248
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	271	1	Y → D in AAG09205. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q43067-1 [UniParc]. Last modified September 26, 2001. Version 2. Checksum: 1D8C4F348D4B820A	FASTA	505	57,991
	10 20 30 40 50 60 MDLLLLEKTL LALFLAAITA ITISKLRGKP FKLPPGPFPV PVFGNWLQVG DDLNHRNLTD 70 80 90 100 110 120 LAKRFAEILL LRMEQRNLVV ISSPELAKEV LHTQGVEFGS RTRNVVFDIF TGKGQDIVFT 130 140 150 160 170 180 VYGEHWRKMR RIMTVPFFTN KVVQQYRFGW ESEAASVVDD VKKNSKASVN GIVIRRRLQL 190 200 210 220 230 240 MMYNIMYRIM FDRRFESEED PLFVKLKALN GERSRLAQSF EYNYGDFIPI LRPFLKGYLK 250 260 270 280 290 300 VCKEVKDRRL QLFKDYFVDE RKKLGSTKST YNEGLKCAID HILDAQKKGE INDDNVLYIV 310 320 330 340 350 360 ENINVAAIET TLWSIEWGIA ELVNHQEIQN KLREEMDKVL GPGHQVTEPD LEKLPYLQAV 370 380 390 400 410 420 IKETLRLRMA IPLLVPHMNL HDAKLGGFDI PAESKILVNA WWLANNPALW KKPEEFRPER 430 440 450 460 470 480 FLEEEAHVEA NGNDFRYLPF GVGRRSCPGI ILALPILGIT IGRLVQNFEL LPPPGQSKID 490 500 TSEKGGQFSL HILKHSTIVA KPRAF

References

[1]	"Molecular characterization of CYP73A9 and CYP82A1 P450 genes involved in plant defense in pea." Whitbred J.M., Schuler M.A. Plant Physiol. 124:47-58(2000) [PubMed: 10982421] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1-497. Strain: cv. Little Marvel. Tissue: Stem.
[2]	"Cloning of wound-induced cytochrome P450 monooxygenases expressed in pea." Frank M.R., Deyneka J.M., Schuler M.A. Plant Physiol. 110:1035-1046(1996) [PubMed: 8819874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-505. Tissue: Stem.
[3]	Schuler M.A. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 208; 394; 404 AND 460.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF175275 Genomic DNA. Translation: AAG09205.1. U29243 mRNA. Translation: AAC49187.2.
PIR	T06522.
3D structure databases
SMR	Q43067. Positions 28-486.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.11. 287.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCMO_PEA

Accession

Primary (citable) accession number: Q43067
Secondary accession number(s): Q9FVK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	September 26, 2001
Last modified:	February 9, 2010
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)