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Protein

Glutamine synthetase root isozyme B

Gene

GS3B

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase root isozyme B (EC:6.3.1.2)
Alternative name(s):
Cytosolic GS3 B
Glutamate--ammonia ligase
Gene namesi
Name:GS3B
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Glutamine synthetase root isozyme BPRO_0000153190Add
BLAST

Interactioni

Subunit structurei

Homooctamer.

Structurei

3D structure databases

ProteinModelPortaliQ43066.
SMRiQ43066. Positions 4-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q43066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLSDLINF NLSDSTEKII AEYIWVGGSG IDIRSKARTL PGPVSDPAKL
60 70 80 90 100
PKWNYDGSST DQAPGKDSEV ILYPQAIFKD PFRRGNNILV ICDVYTPAGE
110 120 130 140 150
PLPTNKRYNA AKIFSHPDVA AEVPWYGIEQ EYTLLQKDIN WPLGWPIGGY
160 170 180 190 200
PGKQGPYYCG IGADKAYGRD IVDAHYKACL FAGINISGIN GEVMPGQWEF
210 220 230 240 250
QVGPSVGISA GDEIWAARYI LERITEISGV VVSFDPKPIP GDWNGAGAHA
260 270 280 290 300
NFSTKSMREN GGYEVIKKAI EKLGLRHKEH IAAYGEGNER RLTGKHETAD
310 320 330 340 350
INVFSWGVAN RGSSIRVGRD TEKDGKGYFE DRRPASNMDP YVVTSMIAET

TILWKKS
Length:357
Mass (Da):39,295
Last modified:October 31, 1996 - v1
Checksum:iF5F09B4BD96912CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28925 Genomic DNA. Translation: AAB03493.1.
PIRiS62712.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28925 Genomic DNA. Translation: AAB03493.1.
PIRiS62712.

3D structure databases

ProteinModelPortaliQ43066.
SMRiQ43066. Positions 4-355.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular evolution of duplicate copies of genes encoding cytosolic glutamine synthetase in Pisum sativum."
    Walker E.L., Weeden N.F., Taylor C.B., Green P., Coruzzi G.M.
    Plant Mol. Biol. 29:1111-1125(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Feltham First.

Entry informationi

Entry nameiGLNA4_PEA
AccessioniPrimary (citable) accession number: Q43066
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 14, 1999
Last sequence update: October 31, 1996
Last modified: January 6, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In pea there are distinct isozymes in leaves, roots and nodules.
Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.