Reviewed, UniProtKB/Swiss-Prot Q43066 (GLNA4_PEA)

Names and origin

Protein names

Recommended name:
    Glutamine synthetase root isozyme B
    EC=6.3.1.2
Alternative name(s):
    Cytosolic GS3 B
    Glutamate--ammonia ligase

Gene names

Name:

GS3B

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
Subunit structure	Homooctamer.
Subcellular location	Cytoplasm.
Miscellaneous	In pea there are distinct isozymes in leaves, roots and nodules. Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).
Sequence similarities	Belongs to the glutamine synthetase family.

Ontologies

Keywords
Biological process	Nitrogen fixation
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Glutamine synthetase root isozyme B		PRO_0000153190

Sequences

	Sequence		Length	Mass (Da)
	Q43066-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F5F09B4BD96912CA	FASTA	357	39,295
	10 20 30 40 50 60 MSSLSDLINF NLSDSTEKII AEYIWVGGSG IDIRSKARTL PGPVSDPAKL PKWNYDGSST 70 80 90 100 110 120 DQAPGKDSEV ILYPQAIFKD PFRRGNNILV ICDVYTPAGE PLPTNKRYNA AKIFSHPDVA 130 140 150 160 170 180 AEVPWYGIEQ EYTLLQKDIN WPLGWPIGGY PGKQGPYYCG IGADKAYGRD IVDAHYKACL 190 200 210 220 230 240 FAGINISGIN GEVMPGQWEF QVGPSVGISA GDEIWAARYI LERITEISGV VVSFDPKPIP 250 260 270 280 290 300 GDWNGAGAHA NFSTKSMREN GGYEVIKKAI EKLGLRHKEH IAAYGEGNER RLTGKHETAD 310 320 330 340 350 INVFSWGVAN RGSSIRVGRD TEKDGKGYFE DRRPASNMDP YVVTSMIAET TILWKKS

References

[1]

"Molecular evolution of duplicate copies of genes encoding cytosolic glutamine synthetase in Pisum sativum." Walker E.L., Weeden N.F., Taylor C.B., Green P., Coruzzi G.M. Plant Mol. Biol. 29:1111-1125(1995) [PubMed: 8616212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Feltham First.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U28925 Genomic DNA. Translation: AAB03493.1.
PIR	S62712.
3D structure databases
SMR	Q43066. Positions 4-355.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.3.1.2. 287.
Family and domain databases
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat. IPR008146. Gln_synth_cat. [Graphical view]
Gene3D	G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
ProDom	PD001057. Gln_synt_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00180. GLNA_1. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNA4_PEA

Accession

Primary (citable) accession number: Q43066

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)