Reviewed, UniProtKB/Swiss-Prot Q43064 (PYRB3_PEA)

Names and origin

Protein names

Recommended name:
    Aspartate carbamoyltransferase 3, chloroplastic
    EC=2.1.3.2
Alternative name(s):
    Aspartate transcarbamylase 3
      Short name=ATCase 3

Gene names

Name:

PYRB3

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.
Enzyme regulation	Allosterically regulated by UMP.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.
Subunit structure	Homotrimer Potential.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 69	69	Chloroplast Potential
	Chain	70 – 391	322	Aspartate carbamoyltransferase 3, chloroplastic		PRO_0000020351

Sequences

	Sequence		Length	Mass (Da)
	Q43064-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5693EBC634FAEBA9	FASTA	391	44,344
	10 20 30 40 50 60 MTASSSLFSC SMHMEVLTPK ISKWPKDFVS CHSKISYVET NYLKSTCYPI SRFLCINNLS 70 80 90 100 110 120 KCDKMIKTRQ RDGIHCFSEG QKFQLDDVIE AQQFDRDILN AIFEIARDME NIERNSPESQ 130 140 150 160 170 180 ILKGYLMATL FYEPSTRTRL SFESAMRRLG GEVLTTENAR EFSSAAKGET LEDTIRTVEG 190 200 210 220 230 240 YSDLIVLRHF ESGAARRAAA IAGIPIVNAG DGPGQHPSQA LLDVYTIERE IGKLDGIKVG 250 260 270 280 290 300 LVGDLANGRT VRSLAYLLAK YKDVKIYFVS PEVVKMKDDI KDYLTSKGVD WEESSDLVEV 310 320 330 340 350 360 ASECDVVYQT RIQKERFGER LDLYEKARGK FIVNQNILNA MQRHAVIMHP LPRLDEITVD 370 380 390 VDADPRAAYF RQAKYGLYIR MALLKLLLVG W

References

[1]	"Characterization of a cDNA encoding a third aspartate transcarbamoylase (pyrB3) from pea." Williamson C.L., To L., Slocum R.D. Plant Gene Register PGR96-063 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Wando. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U05293 mRNA. Translation: AAB67857.1.
PIR	T06425.
3D structure databases
SMR	Q43064. Positions 87-388.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.3.2. 287.
Family and domain databases
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P_bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn_bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
TIGRFAMs	TIGR00670. asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB3_PEA

Accession

Primary (citable) accession number: Q43064

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)