ID   CFI_PUELO               Reviewed;         224 AA.
AC   Q43056;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 45.
DE   RecName: Full=Chalcone--flavonone isomerase;
DE            Short=Chalcone isomerase;
DE            EC=5.5.1.6;
GN   Name=CHI;
OS   Pueraria lobata (Kudzu vine).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Phaseoleae;
OC   Pueraria.
OX   NCBI_TaxID=3893;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND MUTAGENESIS OF CYS-119.
RX   MEDLINE=96410781; PubMed=8812858; DOI=10.1006/prep.1996.0091;
RA   Terai Y., Fujii I., Byun S.-H., Nakajima O., Hakamatsuka T.,
RA   Ebizuka Y., Sankawa U.;
RT   "Cloning of chalcone-flavanone isomerase cDNA from Pueraria lobata and
RT   its overexpression in Escherichia coli.";
RL   Protein Expr. Purif. 8:183-190(1996).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed
CC       chalcone) into naringenin.
CC   -!- CATALYTIC ACTIVITY: A chalcone = a flavanone.
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid
CC       biosynthesis.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of
CC       which are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family.
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DR   EMBL; D63577; BAA09795.1; -; mRNA.
DR   HSSP; P28012; 1EYP.
DR   SMR; Q43056; 9-219.
DR   BRENDA; 5.5.1.6; 2266.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR003466; Chalcone_isomerase_subgr.
DR   Gene3D; G3DSA:3.50.70.10; Chalcone; 1.
DR   Pfam; PF02431; Chalcone; 1.
PE   1: Evidence at protein level;
KW   Flavonoid biosynthesis; Isomerase.
FT   CHAIN         1    224       Chalcone--flavonone isomerase.
FT                                /FTId=PRO_0000166440.
FT   BINDING      53     53       Substrate (By similarity).
FT   BINDING     118    118       Substrate (By similarity).
FT   BINDING     195    195       Substrate (By similarity).
FT   SITE        111    111       Important for catalytic activity (By
FT                                similarity).
FT   MUTAGEN     119    119       C->A: No loss of activity.
SQ   SEQUENCE   224 AA;  23804 MW;  6C45CC742D76AD1E CRC64;
     MAAAAAVATI SAVQVEFLEF PAVVTSPASG RTYFLGGAGE RGLTIEGKFI KFTGIGVYLE
     DKAVSSLAAK WKGKPSEELV ETLDFYRDII SGPFEKLIRG SKILPLSGVE YSKKVMENCV
     AHMKSVGTYG DAEAAAIEKF AEAFKNVNFQ PGATVFYRQS PDGVLGLSFS EDVTIPDNEA
     AVIENKAVSA AVLETMIGEH AVSPDLKRSL ASRLPAVLSH GIIV
//