Reviewed, UniProtKB/Swiss-Prot Q43056 (CFI_PUELO)

Names and origin

Protein names

Recommended name:
    Chalcone--flavonone isomerase
      Short name=Chalcone isomerase
    EC=5.5.1.6

Gene names

Name:

CHI

Organism

Pueraria lobata (Kudzu vine)

Taxonomic identifier

3893 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Pueraria

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin. Ref.1
Catalytic activity	A chalcone = a flavanone.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Miscellaneous	Part of the biosynthetic pathway for all classes of flavonoids, a large class of secondary plant metabolites, many of which are brightly colored.
Sequence similarities	Belongs to the chalcone isomerase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chalcone isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Chalcone--flavonone isomerase		PRO_0000166440
Sites
	Binding site	53	1	Substrate By similarity
	Binding site	118	1	Substrate By similarity
	Binding site	195	1	Substrate By similarity
	Site	111	1	Important for catalytic activity By similarity
Experimental info
	Mutagenesis	119	1	C → A: No loss of activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q43056-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6C45CC742D76AD1E	FASTA	224	23,804
	10 20 30 40 50 60 MAAAAAVATI SAVQVEFLEF PAVVTSPASG RTYFLGGAGE RGLTIEGKFI KFTGIGVYLE 70 80 90 100 110 120 DKAVSSLAAK WKGKPSEELV ETLDFYRDII SGPFEKLIRG SKILPLSGVE YSKKVMENCV 130 140 150 160 170 180 AHMKSVGTYG DAEAAAIEKF AEAFKNVNFQ PGATVFYRQS PDGVLGLSFS EDVTIPDNEA 190 200 210 220 AVIENKAVSA AVLETMIGEH AVSPDLKRSL ASRLPAVLSH GIIV

References

[1]

"Cloning of chalcone-flavanone isomerase cDNA from Pueraria lobata and its overexpression in Escherichia coli." Terai Y., Fujii I., Byun S.-H., Nakajima O., Hakamatsuka T., Ebizuka Y., Sankawa U. Protein Expr. Purif. 8:183-190(1996) [PubMed: 8812858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-119.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D63577 mRNA. Translation: BAA09795.1.
3D structure databases
HSSP	HSSP built from PDB template 1EYP based on UniProtKB P28012.
SMR	Q43056. Positions 9-219.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.5.1.6. 2266.
Family and domain databases
InterPro	IPR016088. Chalcone_isomerase_3-sand. IPR003466. Chalcone_isomerase_subgr. [Graphical view]
Gene3D	G3DSA:3.50.70.10. Chalcone. 1 hit.
Pfam	PF02431. Chalcone. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CFI_PUELO

Accession

Primary (citable) accession number: Q43056

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)