ID   TCMO_POPKI              Reviewed;         505 AA.
AC   Q43054; O04989; Q40906; Q43044;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   24-MAR-2009, entry version 53.
DE   RecName: Full=Trans-cinnamate 4-monooxygenase;
DE            EC=1.14.13.11;
DE   AltName: Full=Cinnamic acid 4-hydroxylase;
DE            Short=CA4H;
DE            Short=C4H;
DE   AltName: Full=P450C4H;
DE   AltName: Full=Cytochrome P450 73;
GN   Name=CYP73A16; Synonyms=CYP73;
OS   Populus kitakamiensis (Aspen) (Populus sieboldii x Populus
OS   grandidentata).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Malpighiales; Salicaceae; Saliceae; Populus.
OX   NCBI_TaxID=34292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=cv. Y-63;
RX   MEDLINE=97141312; PubMed=8987656;
RA   Kawai S., Mori A., Shiokawa T., Kajita S., Katayama Y., Morohoshi N.;
RT   "Isolation and analysis of cinnamic acid 4-hydroxylase homologous
RT   genes from a hybrid aspen, Populus kitakamiensis.";
RL   Biosci. Biotechnol. Biochem. 60:1586-1597(1996).
CC   -!- FUNCTION: Controls carbon flux to pigments essential for
CC       pollination or UV protection, to numerous pytoalexins synthesized
CC       by plants when challenged by pathogens, and to lignins.
CC   -!- CATALYTIC ACTIVITY: Trans-cinnamate + NADPH + O(2) = 4-
CC       hydroxycinnamate + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Phenylpropanoid metabolism; 4-coumaric acid biosynthesis;
CC       4-coumaric acid from trans-cinnamic acid: step 1/1.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; D82812; BAA11576.1; -; Genomic_DNA.
DR   EMBL; D82815; BAA11579.1; -; mRNA.
DR   BRENDA; 1.14.13.11; 280164.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0016710; F:trans-cinnamate 4-monooxygenase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Metal-binding; Monooxygenase; NADP; Oxidoreductase.
FT   CHAIN         1    505       Trans-cinnamate 4-monooxygenase.
FT                                /FTId=PRO_0000052251.
FT   METAL       447    447       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   505 AA;  57973 MW;  FA97C0FA9B138151 CRC64;
     MDLLLLEKTL LGSFVAVLVA ILVSKLRGKR FKLPPGPLPV PVFGNWLQVG DDLNHRNLTD
     LAKKFGDIFL LRMGQRNLVV VSSPDLSKEV LHTQGVEFGS RTRNVVFDIF TGKGQDMVFT
     VYGEHWRKMR RIMTVPFFTN KVVQQYRYGW EEEAAQVVED VKKNPEAATN GIVLRRRLQL
     MMYNNMYRIM FDRRFESEDD PLFNKLKALN GERSRLAQSF DYNYGDFIPI LRPFLRGYLK
     ICQEVKERRL QLFKDYFVDE RKKLASTKNM SNEGLKCAID HILDAQKKGE INEDNVLYIV
     ENINVAAIET TLWSIEWGIA ELVNHPEIQK KLRHELDTLL GPGHQITEPD TYKLPYLNAV
     VKETLRLRMA IPLLVPHMNL HDAKLGGFDI PAESKILVNA WWLANNPAHW KNPEEFRPER
     FLEEGAKVEA NGNDFRYLPF GVGRRSCPGI ILALPILGIT LGRLVQNFEL LPPPGQSKID
     TSEKGGQFSL HILKHSTIVA KPRSF
//