ID   PAL2_POPKI              Reviewed;         710 AA.
AC   Q43052;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 58.
DE   RecName: Full=Phenylalanine ammonia-lyase G2B;
DE            EC=4.3.1.24;
GN   Name=PALG2B;
OS   Populus kitakamiensis (Aspen) (Populus sieboldii x Populus
OS   grandidentata).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Malpighiales; Salicaceae; Saliceae; Populus.
OX   NCBI_TaxID=34292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=96011759; PubMed=7548831; DOI=10.1007/BF00032674;
RA   Osakabe Y., Osakabe K., Kawai S., Katayama Y., Morohoshi N.;
RT   "Characterization of the structure and determination of mRNA levels of
RT   the phenylalanine ammonia-lyase gene family from Populus
RT   kitakamiensis.";
RL   Plant Mol. Biol. 28:1133-1141(1995).
CC   -!- FUNCTION: This is a key enzyme of plant metabolism catalyzing the
CC       first reaction in the biosynthesis from L-phenylalanine of a wide
CC       variety of natural products based on the phenylpropane skeleton.
CC   -!- CATALYTIC ACTIVITY: L-phenylalanine = trans-cinnamate + ammonia.
CC   -!- PATHWAY: Phenylpropanoid metabolism; cinnamic acid biosynthesis;
CC       trans-cinnamic acid from L-phenylalanine: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- PTM: Contains an active site 4-methylidene-imidazol-5-one (MIO),
CC       which is formed autocatalytically by cyclization and dehydration
CC       of residues Ala-Ser-Gly (By similarity).
CC   -!- SIMILARITY: Belongs to the PAL/histidase family.
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DR   EMBL; D43802; BAA07860.1; -; Genomic_DNA.
DR   HSSP; P21310; 1GKM.
DR   SMR; Q43052; 20-710.
DR   BRENDA; 4.3.1.5; 280164.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016211; F:ammonia ligase activity; IEA:InterPro.
DR   GO; GO:0016841; F:ammonia-lyase activity; IEA:InterPro.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   GO; GO:0006559; P:L-phenylalanine catabolic process; IEA:InterPro.
DR   GO; GO:0009698; P:phenylpropanoid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001106; Phe/His_NH3-lyase.
DR   InterPro; IPR005922; Phe_NH3-lyase.
DR   Pfam; PF00221; PAL; 1.
DR   TIGRFAMs; TIGR01226; phe_am_lyase; 1.
DR   PROSITE; PS00488; PAL_HISTIDASE; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Lyase; Phenylpropanoid metabolism.
FT   CHAIN         1    710       Phenylalanine ammonia-lyase G2B.
FT                                /FTId=PRO_0000215414.
FT   MOD_RES     198    198       2,3-didehydroalanine (Ser) (By
FT                                similarity).
FT   CROSSLNK    197    199       5-imidazolinone (Ala-Gly) (By
FT                                similarity).
SQ   SEQUENCE   710 AA;  77585 MW;  B81ADAA0DF641080 CRC64;
     MEFCQDSRNG NGSPGFNTND PLNWGMAAES LKGSHLDEVK RMIEEYRNPV VKLGGETLTI
     GQVTAIASRD VGVMVELSEE ARAGVKASSD WVMDSMSKGT DSYGVTTGFG ATSHRRTKQG
     GELQKELIRF LNAGIFGNGT ESSHTLPRSA TRAAMLVRTN TLLQGYSGIR FEMLEAITKM
     INHNITPCLP LRGTITASGD LVPLSYIAGL LTGRPNSKAV GPNGEPLTPA EAFTQAGIDG
     GFFELQPKEG LALVNGTAVG SGLASMVLFE ANVLAILSEV LSAIFAEVMQ GKPEFTDHLT
     HKLKHHPGQI VAAAIMEHIL DGSAYVKEAQ KLHEIDPLQK PKQDRHALRT SPQWLGPLIE
     VIRTSTKMIE REINSVNDNP LIDVSRNKAL HGGNFQGTPI GVSMDNTRLA IASIGKLMFA
     QFSELVNDLY NNGLPSNLTG GRNPSLDYGF KGAEIAMASY CSELQFLDQS CTNHVQSAEQ
     HNQDVNSLGL ISSRKTAEAI DILKLMSTTF LVGLCHSVDL RHIEENLKNT VKISVSQLPR
     VLTMGFNGEL HPSRFCEKDL LKVVDREHVF SYIDDPCSAT YPLMQKLRQV LVEHALVNGE
     KVRNSTTSIF QKIGSFEEEL KTLLPKEVES ARLEVENGNP AIPNRIKECR SYPLYKFVRE
     ELGTSLLTGE KVKSPGEEFD KVFTAICAGK LIDPLLECLK EWDGAPLPIC
//