Reviewed, UniProtKB/Swiss-Prot Q43052 (PAL2_POPKI)

Names and origin

Protein names

Recommended name:
    Phenylalanine ammonia-lyase G2B
    EC=4.3.1.24

Gene names

Name:

PALG2B

Organism

Populus kitakamiensis (Aspen) (Populus sieboldii x Populus grandidentata)

Taxonomic identifier

34292 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Salicaceae › Saliceae › Populus

Protein attributes

Sequence length	710 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.
Catalytic activity	L-phenylalanine = trans-cinnamate + ammonia.
Pathway	Phenylpropanoid metabolism; cinnamic acid biosynthesis; trans-cinnamic acid from L-phenylalanine: step 1/1.
Subcellular location	Cytoplasm Probable.
Post-translational modification	Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly By similarity.
Sequence similarities	Belongs to the PAL/histidase family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Cellular component	Cytoplasm
Molecular function	Lyase
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: InterPro biosynthetic process Inferred from electronic annotation. Source: InterPro phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ammonia ligase activity Inferred from electronic annotation. Source: InterPro ammonia-lyase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 710	710	Phenylalanine ammonia-lyase G2B		PRO_0000215414
Amino acid modifications
	Modified residue	198	1	2,3-didehydroalanine (Ser) By similarity
	Cross-link	197 ↔ 199		5-imidazolinone (Ala-Gly) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43052-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B81ADAA0DF641080	FASTA	710	77,585
	10 20 30 40 50 60 MEFCQDSRNG NGSPGFNTND PLNWGMAAES LKGSHLDEVK RMIEEYRNPV VKLGGETLTI 70 80 90 100 110 120 GQVTAIASRD VGVMVELSEE ARAGVKASSD WVMDSMSKGT DSYGVTTGFG ATSHRRTKQG 130 140 150 160 170 180 GELQKELIRF LNAGIFGNGT ESSHTLPRSA TRAAMLVRTN TLLQGYSGIR FEMLEAITKM 190 200 210 220 230 240 INHNITPCLP LRGTITASGD LVPLSYIAGL LTGRPNSKAV GPNGEPLTPA EAFTQAGIDG 250 260 270 280 290 300 GFFELQPKEG LALVNGTAVG SGLASMVLFE ANVLAILSEV LSAIFAEVMQ GKPEFTDHLT 310 320 330 340 350 360 HKLKHHPGQI VAAAIMEHIL DGSAYVKEAQ KLHEIDPLQK PKQDRHALRT SPQWLGPLIE 370 380 390 400 410 420 VIRTSTKMIE REINSVNDNP LIDVSRNKAL HGGNFQGTPI GVSMDNTRLA IASIGKLMFA 430 440 450 460 470 480 QFSELVNDLY NNGLPSNLTG GRNPSLDYGF KGAEIAMASY CSELQFLDQS CTNHVQSAEQ 490 500 510 520 530 540 HNQDVNSLGL ISSRKTAEAI DILKLMSTTF LVGLCHSVDL RHIEENLKNT VKISVSQLPR 550 560 570 580 590 600 VLTMGFNGEL HPSRFCEKDL LKVVDREHVF SYIDDPCSAT YPLMQKLRQV LVEHALVNGE 610 620 630 640 650 660 KVRNSTTSIF QKIGSFEEEL KTLLPKEVES ARLEVENGNP AIPNRIKECR SYPLYKFVRE 670 680 690 700 710 ELGTSLLTGE KVKSPGEEFD KVFTAICAGK LIDPLLECLK EWDGAPLPIC

References

[1]

"Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis." Osakabe Y., Osakabe K., Kawai S., Katayama Y., Morohoshi N. Plant Mol. Biol. 28:1133-1141(1995) [PubMed: 7548831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D43802 Genomic DNA. Translation: BAA07860.1.
3D structure databases
HSSP	HSSP built from PDB template 1GKM based on UniProtKB P21310.
SMR	Q43052. Positions 20-710.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.3.1.5. 280164.
Family and domain databases
InterPro	IPR001106. Phe/His_NH3-lyase. IPR005922. Phe_NH3-lyase. [Graphical view]
Pfam	PF00221. PAL. 1 hit. [Graphical view]
TIGRFAMs	TIGR01226. phe_am_lyase. 1 hit.
PROSITE	PS00488. PAL_HISTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PAL2_POPKI

Accession

Primary (citable) accession number: Q43052

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)