ID   COMT1_POPKI             Reviewed;         365 AA.
AC   Q43046;
DT   11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   20-JAN-2009, entry version 51.
DE   RecName: Full=Caffeic acid 3-O-methyltransferase 1;
DE            EC=2.1.1.68;
DE   AltName: Full=S-adenosysl-L-methionine:caffeic acid 3-O-methyltransferase 1;
DE            Short=CAOMT-1;
DE            Short=COMT-1;
GN   Name=HOMT1;
OS   Populus kitakamiensis (Aspen) (Populus sieboldii x Populus
OS   grandidentata).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Malpighiales; Salicaceae; Saliceae; Populus.
OX   NCBI_TaxID=34292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Hayakawa T., Nanto K., Kawai S., Katayama Y., Morohoshi N.;
RT   "Molecular cloning and tissue-specific expression of two genes that
RT   encode caffeic acid O-methyltransferases from Populus kitakamiensis.";
RL   Plant Sci. 113:157-165(1996).
CC   -!- FUNCTION: Catalyzes the conversion of caffeic acid to ferulic acid
CC       and of 5-hydroxyferulic acid to sinapic acid. The resulting
CC       products may subsequently be converted to the corresponding
CC       alcohols that are incorporated into lignins.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + 3,4-dihydroxy-trans-
CC       cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-
CC       cinnamate.
CC   -!- PATHWAY: Aromatic compound metabolism; phenylpropanoid
CC       biosynthesis.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. Type 2
CC       family. COMT subfamily.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; D49710; BAA08558.1; -; Genomic_DNA.
DR   HSSP; P28002; 1KYZ.
DR   SMR; Q43046; 5-364.
DR   BRENDA; 2.1.1.68; 280164.
DR   GO; GO:0047763; F:caffeate O-methyltransferase activity; IEA:EC.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0009809; P:lignin biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR016461; O-MeTrfase_COMT_euk.
DR   InterPro; IPR001077; O_MeTrfase_2.
DR   InterPro; IPR012967; Plant_MeTrfase_dimerisation.
DR   InterPro; IPR011991; Wing_hlx_DNA_bd.
DR   Gene3D; G3DSA:1.10.10.10; Wing_hlx_DNA_bd; 1.
DR   Pfam; PF08100; Dimerisation; 1.
DR   Pfam; PF00891; Methyltransf_2; 1.
DR   PIRSF; PIRSF005739; O-mtase; 1.
PE   3: Inferred from homology;
KW   Lignin biosynthesis; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN         1    365       Caffeic acid 3-O-methyltransferase 1.
FT                                /FTId=PRO_0000063207.
FT   REGION      130    136       Substrate binding (By similarity).
FT   REGION      162    180       Substrate binding (By similarity).
FT   ACT_SITE    269    269       Proton acceptor (By similarity).
FT   BINDING     208    208       S-adenosyl-L-methionine; via carbonyl
FT                                oxygen (By similarity).
FT   BINDING     231    231       S-adenosyl-L-methionine (By similarity).
FT   BINDING     251    251       S-adenosyl-L-methionine (By similarity).
FT   BINDING     252    252       S-adenosyl-L-methionine; via amide
FT                                nitrogen (By similarity).
FT   BINDING     265    265       S-adenosyl-L-methionine (By similarity).
SQ   SEQUENCE   365 AA;  39791 MW;  D6005B10FE55B83C CRC64;
     MGSTGETQMT PTQVSDEEAH LFAMQLASAS VLPMILKTAI ELDLLEIMAK AGPGAFLSTS
     EIASHLPTKN PDAPVMLDRI LRLLASYSIL TCSLKDLPDG KVERLYGLAP VCKFLTKNED
     GVSVSPLCLM NQDKVLMESW YYLKDAILEG GIPFNKAYGM TAFEYHGTDP RFNKVFNKGM
     SDHSTITMKK ILETYKGFEG LTSLVDVGGG TGAVVNTIVS KYPSIKGINF DLPHVIEDAP
     SYPGVEHVGG DMFVSVPNAD AVFMKWICHD WSDAHCLKFL KNCYDALPEN GKVILVECIL
     PVAPDTSLAT KGVVHVDVIM LAHNPGGKER TEKEFEGLAN GAGFQGFEVM CCAFNTHVIE
     FRKKA
//