Reviewed, UniProtKB/Swiss-Prot Q43046 (COMT1_POPKI)

Names and origin

Protein names

Recommended name:
    Caffeic acid 3-O-methyltransferase 1
    EC=2.1.1.68
Alternative name(s):
    S-adenosysl-L-methionine:caffeic acid 3-O-methyltransferase 1
      Short name=CAOMT-1
      Short name=COMT-1

Gene names

Name:

HOMT1

Organism

Populus kitakamiensis (Aspen) (Populus sieboldii x Populus grandidentata)

Taxonomic identifier

34292 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Salicaceae › Saliceae › Populus

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid. The resulting products may subsequently be converted to the corresponding alcohols that are incorporated into lignins.
Catalytic activity	S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate.
Pathway	Aromatic compound metabolism; phenylpropanoid biosynthesis.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. Type 2 family. COMT subfamily.

Ontologies

Keywords
Biological process	Lignin biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	lignin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	caffeate O-methyltransferase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Caffeic acid 3-O-methyltransferase 1		PRO_0000063207
Regions
	Region	130 – 136	7	Substrate binding By similarity
	Region	162 – 180	19	Substrate binding By similarity
Sites
	Active site	269	1	Proton acceptor By similarity
	Binding site	208	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	231	1	S-adenosyl-L-methionine By similarity
	Binding site	251	1	S-adenosyl-L-methionine By similarity
	Binding site	252	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	265	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43046-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D6005B10FE55B83C	FASTA	365	39,791
	10 20 30 40 50 60 MGSTGETQMT PTQVSDEEAH LFAMQLASAS VLPMILKTAI ELDLLEIMAK AGPGAFLSTS 70 80 90 100 110 120 EIASHLPTKN PDAPVMLDRI LRLLASYSIL TCSLKDLPDG KVERLYGLAP VCKFLTKNED 130 140 150 160 170 180 GVSVSPLCLM NQDKVLMESW YYLKDAILEG GIPFNKAYGM TAFEYHGTDP RFNKVFNKGM 190 200 210 220 230 240 SDHSTITMKK ILETYKGFEG LTSLVDVGGG TGAVVNTIVS KYPSIKGINF DLPHVIEDAP 250 260 270 280 290 300 SYPGVEHVGG DMFVSVPNAD AVFMKWICHD WSDAHCLKFL KNCYDALPEN GKVILVECIL 310 320 330 340 350 360 PVAPDTSLAT KGVVHVDVIM LAHNPGGKER TEKEFEGLAN GAGFQGFEVM CCAFNTHVIE FRKKA

References

[1]	"Molecular cloning and tissue-specific expression of two genes that encode caffeic acid O-methyltransferases from Populus kitakamiensis." Hayakawa T., Nanto K., Kawai S., Katayama Y., Morohoshi N. Plant Sci. 113:157-165(1996) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D49710 Genomic DNA. Translation: BAA08558.1.
3D structure databases
HSSP	HSSP built from PDB template 1KYZ based on UniProtKB P28002.
SMR	Q43046. Positions 5-364.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.68. 280164.
Family and domain databases
InterPro	IPR016461. O-MeTrfase_COMT_euk. IPR001077. O_MeTrfase_2. IPR012967. Plant_MeTrfase_dimerisation. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF08100. Dimerisation. 1 hit. PF00891. Methyltransf_2. 1 hit. [Graphical view]
PIRSF	PIRSF005739. O-mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COMT1_POPKI

Accession

Primary (citable) accession number: Q43046

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	November 1, 1996
Last modified:	January 20, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)