Reviewed, UniProtKB/Swiss-Prot Q43043 (PME_PETIN)

Names and origin

Protein names

Recommended name:
    Pectinesterase
      Short name=PE
    EC=3.1.1.11
Alternative name(s):
    Pectin methylesterase

Gene names

Name:

PPE1

Organism

Petunia integrifolia (Violet-flowered petunia) (Petunia inflata)

Taxonomic identifier

4103 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Petunioideae › Petunia

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a role in pollen germination and/or tube growth.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted › cell wall Probable.
Tissue specificity	Pollen, and at much lower levels in pistils and petals.
Developmental stage	Appears first in early bicellular pollen, peaking in mature pollen.
Sequence similarities	Belongs to the pectinesterase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 374	343	Pectinesterase		PRO_0000023495
Sites
	Active site	186	1	Proton donor By similarity
	Active site	207	1	Nucleophile By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	163	1	Substrate By similarity
	Binding site	275	1	Substrate By similarity
	Binding site	277	1	Substrate By similarity
	Site	185	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	124	1	N-linked (GlcNAc...) Potential
	Glycosylation	230	1	N-linked (GlcNAc...) Potential
	Glycosylation	303	1	N-linked (GlcNAc...) Potential
	Disulfide bond	200 ↔ 220		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43043-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BC52DC4501B57490	FASTA	374	41,467
	10 20 30 40 50 60 MVKLLNSTRE LSINALSMLN SFGDMVAQAT GLNRKLLTTD SSDATARRLL QISNAKPNAT 70 80 90 100 110 120 VALDGSGQYK TIKEALDAVP KKNTEPFIIF IKAGVYKEYI DIPKSMTNVV LIGEGPTKTK 130 140 150 160 170 180 ITGNKSVKDG PSTFHTTTVG VNGANFVAKN IGFENTAGPE KEQAVALRVS ADKAIIYNCQ 190 200 210 220 230 240 IDGYQDTLYV HTYRQFYRDC TITGTVDFIF GNGEAVLQNC KVIVRKPAQN QSCMVTAQGR 250 260 270 280 290 300 TEPIQKGAIV LQNCEIKPDT DYFSLSPPSK TYLGRPWKEY SRTIIMQSYI DKFIEPEGWA 310 320 330 340 350 360 PWNITNFGRD TSYYAEYQNR GPGAALDKRI TWKGFQKGFT GEAAQKFTAG VYINNDENWL 370 QKANVPYEAG MMKV

References

[1]	"Characterization of a pollen-expressed gene encoding a putative pectin esterase of Petunia inflata." Mu J.H., Stains J., Kao T.H. Plant Mol. Biol. 25:539-544(1994) [PubMed: 8049376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Pollen.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L27101 Genomic DNA. Translation: AAA33714.1.
PIR	S78041.
3D structure databases
HSSP	HSSP built from PDB template 1GQ8 based on UniProtKB P83218.
ModBase	Search...
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
PROSITE	PS00800. PECTINESTERASE_1. 1 hit. PS00503. PECTINESTERASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME_PETIN

Accession

Primary (citable) accession number: Q43043

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	September 22, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)