ID   TCMO_PETCR              Reviewed;         506 AA.
AC   Q43033;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   05-MAY-2009, entry version 52.
DE   RecName: Full=Trans-cinnamate 4-monooxygenase;
DE            EC=1.14.13.11;
DE   AltName: Full=Cinnamic acid 4-hydroxylase;
DE            Short=CA4H;
DE            Short=C4H;
DE   AltName: Full=P450C4H;
DE   AltName: Full=Cytochrome P450 73;
GN   Name=CYP73A10; Synonyms=CYP73;
OS   Petroselinum crispum (Parsley) (Petroselinum hortense).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; campanulids; Apiales; Apiaceae; Apioideae;
OC   apioid superclade; Apium clade; Petroselinum.
OX   NCBI_TaxID=4043;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=95320184; PubMed=7597051; DOI=10.1073/pnas.92.13.5905;
RA   Logemann E., Parniske M., Hahlbrock K.;
RT   "Modes of expression and common structural features of the complete
RT   phenylalanine ammonia-lyase gene family in parsley.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:5905-5909(1995).
CC   -!- FUNCTION: Controls carbon flux to pigments essential for
CC       pollination or UV protection, to numerous pytoalexins synthesized
CC       by plants when challenged by pathogens, and to lignins.
CC   -!- CATALYTIC ACTIVITY: Trans-cinnamate + NADPH + O(2) = 4-
CC       hydroxycinnamate + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Phenylpropanoid metabolism; 4-coumaric acid biosynthesis;
CC       4-coumaric acid from trans-cinnamic acid: step 1/1.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; L38898; AAC41660.1; -; mRNA.
DR   PIR; T14907; T14907.
DR   BRENDA; 1.14.13.11; 2662.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0016710; F:trans-cinnamate 4-monooxygenase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Metal-binding; Monooxygenase; NADP; Oxidoreductase.
FT   CHAIN         1    506       Trans-cinnamate 4-monooxygenase.
FT                                /FTId=PRO_0000052249.
FT   METAL       448    448       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   506 AA;  58048 MW;  32F00EE959D69CCF CRC64;
     MMDFVLLEKA LLGLFIATIV AITISKLRGK KLKLPPGPIP VPVFGNWLQV GDDLNQRNLV
     DYAKKFGDLF MLRMGQRNLV VVSSPELAKD VLHTQGVEFG SRTRNVVFDI FTGKGQDMVF
     TVYSEHWRKM RRIMTVPFFT NKVVQQYRFG WEDEAARVVE DVKANPEAAT NGIVLRNRLQ
     LLMYNNMYRI MFDRRFESVD DPLFLKLKAL NGERSRLAQS FEYHFGDFIP ILRPFLRGYL
     KLCQEIKDKR LKLFKDYFVD ERKKLESIKS VDNNSLKCAI DHIIEAQQKG EINEDNVLYI
     VENINVAAIE TTLWSIEWGI AELVNNPEIQ KKLRHELDTV LGAGVQICEP DVQKLPYLQA
     VIKETLRYRM AIPLLVPHMN LHDAKLAGYD IPAESKILVN AWWLANNPAH WNKPDEFRPE
     RFLEEESKVE ANGNDFKYIP FGVGRRSCPG IILALPILGI VIGRLVQNFE LLPPPGQSKI
     DTAEKGGQFS LQILKHSTIV CKPRSL
//