Reviewed, UniProtKB/Swiss-Prot Q43033 (TCMO_PETCR)

Names and origin

Protein names

Recommended name:
    Trans-cinnamate 4-monooxygenase
    EC=1.14.13.11
Alternative name(s):
    Cinnamic acid 4-hydroxylase
      Short name=CA4H
      Short name=C4H
    P450C4H
    Cytochrome P450 73

Gene names

Name:	CYP73A10
Synonyms:	CYP73

Organism

Petroselinum crispum (Parsley) (Petroselinum hortense)

Taxonomic identifier

4043 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Apiales › Apiaceae › Apioideae › apioid superclade › Apium clade › Petroselinum

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.
Catalytic activity	Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Phenylpropanoid metabolism; 4-coumaric acid biosynthesis; 4-coumaric acid from trans-cinnamic acid: step 1/1.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro trans-cinnamate 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Trans-cinnamate 4-monooxygenase		PRO_0000052249
Sites
	Metal binding	448	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43033-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 32F00EE959D69CCF	FASTA	506	58,048
	10 20 30 40 50 60 MMDFVLLEKA LLGLFIATIV AITISKLRGK KLKLPPGPIP VPVFGNWLQV GDDLNQRNLV 70 80 90 100 110 120 DYAKKFGDLF MLRMGQRNLV VVSSPELAKD VLHTQGVEFG SRTRNVVFDI FTGKGQDMVF 130 140 150 160 170 180 TVYSEHWRKM RRIMTVPFFT NKVVQQYRFG WEDEAARVVE DVKANPEAAT NGIVLRNRLQ 190 200 210 220 230 240 LLMYNNMYRI MFDRRFESVD DPLFLKLKAL NGERSRLAQS FEYHFGDFIP ILRPFLRGYL 250 260 270 280 290 300 KLCQEIKDKR LKLFKDYFVD ERKKLESIKS VDNNSLKCAI DHIIEAQQKG EINEDNVLYI 310 320 330 340 350 360 VENINVAAIE TTLWSIEWGI AELVNNPEIQ KKLRHELDTV LGAGVQICEP DVQKLPYLQA 370 380 390 400 410 420 VIKETLRYRM AIPLLVPHMN LHDAKLAGYD IPAESKILVN AWWLANNPAH WNKPDEFRPE 430 440 450 460 470 480 RFLEEESKVE ANGNDFKYIP FGVGRRSCPG IILALPILGI VIGRLVQNFE LLPPPGQSKI 490 500 DTAEKGGQFS LQILKHSTIV CKPRSL

References

[1]	"Modes of expression and common structural features of the complete phenylalanine ammonia-lyase gene family in parsley." Logemann E., Parniske M., Hahlbrock K. Proc. Natl. Acad. Sci. U.S.A. 92:5905-5909(1995) [PubMed: 7597051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L38898 mRNA. Translation: AAC41660.1.
PIR	T14907.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.11. 2662.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCMO_PETCR

Accession

Primary (citable) accession number: Q43033

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 5, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)