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Q43011 (ASNS2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Asparagine synthetase [glutamine-hydrolyzing] 2

EC=6.3.5.4
Alternative name(s):
Glutamine-dependent asparagine synthetase 2
Gene names
Ordered Locus Names:Os06g0265000, LOC_Os06g15420
ORF Names:OJ1001_B06.12
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length591 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem By similarity.

Catalytic activity

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway

Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.

Tissue specificity

Expressed in companion cells of leaf sheath vascular bundles, and phloem-parenchyma cells, nucellar projections and nucellar epidermis of dorsal vascular bundles of grains. Ref.7

Sequence similarities

Contains 1 asparagine synthetase domain.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 591590Asparagine synthetase [glutamine-hydrolyzing] 2
PRO_0000056925

Regions

Domain2 – 185184Glutamine amidotransferase type-2
Domain193 – 516324Asparagine synthetase
Nucleotide binding341 – 3422ATP By similarity
Region50 – 545Glutamine binding By similarity
Region75 – 773Glutamine binding By similarity

Sites

Active site21For GATase activity By similarity
Binding site981Glutamine By similarity
Binding site2311ATP; via carbonyl oxygen By similarity
Binding site2671ATP; via amide nitrogen and carbonyl oxygen By similarity
Site3431Important for beta-aspartyl-AMP intermediate formation By similarity

Sequences

Sequence LengthMass (Da)Tools
Q43011 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 3126C674724E4A3D

FASTA59166,227
        10         20         30         40         50         60 
MCGILAVLGV ADVSLAKRSR IIELSRRLRH RGPDWSGIHC YQDCYLAHQR LAIVDPTSGD 

        70         80         90        100        110        120 
QPLYNEDKSV VVTVNGEIYN HEELKANLKS HKFQTASDCE VIAHLYEEYG EEFVDMLDGM 

       130        140        150        160        170        180 
FAFVLLDTRD KSFIAARDAI GICPLYMGWG LDGSVWFSSE MKALSDDCER FISFPPGHLY 

       190        200        210        220        230        240 
SSKTGGLRRW YNPPWFSESI PSTPYNPLLL RQSFEKAIIK RLMTDVPFGV LLSGGLDSSL 

       250        260        270        280        290        300 
VASVVSRHLA EAKVAAQWGN KLHTFCIGLK GSPDLRAAKE VADYLGTVHH ELHFTVQEGI 

       310        320        330        340        350        360 
DALEEVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKMVL SGEGSDEIFG GYLYFHKAPN 

       370        380        390        400        410        420 
KKEFHEETCR KIKALHLYDC LRANKSTSAW GVEARVPFLD KNFINVAMDI DPEWKMIKRD 

       430        440        450        460        470        480 
LGRIEKWVLR NAFDDEEKPY LPKHILYRQK EQFSDGVGYS WIDGLKDHAN EHVSDSMMMN 

       490        500        510        520        530        540 
ASFVYPENTP VTKEAYYYRT IFEKFFPKNA ARLTVPGGPS VACSTAKAVE WDAAWSKNLD 

       550        560        570        580        590 
PSGRAALGVH DAAYEDTLQK SPASANPVLD NGFGPALGES MVKTVASATA V 

« Hide

References

« Hide 'large scale' references
[1]Sueyoshi K., Kawachi T., Nakajima A., Yamagata H., Sugimoto T., Iwasaki T., Oji Y.
Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Nipponbare.
Tissue: Callus.
[2]"Nucleotide sequence of cDNA encoding asparagine synthetase from rice callus."
Watanabe K., Higuchi T., Sakai T., Yamaya T.
Plant Gene Register PGR96-020
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Nipponbare.
Tissue: Callus.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[5]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[6]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[7]"Organ and cellular localization of asparagine synthetase in rice plants."
Nakano K., Suzuki T., Hayakawa T., Yamaya T.
Plant Cell Physiol. 41:874-880(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U55873 mRNA. Translation: AAB03991.1.
D83378 mRNA. Translation: BAA18951.1.
AP005382 Genomic DNA. Translation: BAD54377.1.
AP008212 Genomic DNA. Translation: BAF19254.1.
CM000143 Genomic DNA. Translation: EEE65484.1.
AK100247 mRNA. Translation: BAG94512.1.
PIRT03602.
RefSeqNP_001057340.1. NM_001063875.1.
UniGeneOs.135.

3D structure databases

ProteinModelPortalQ43011.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC44.976.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS06T0265000-01; OS06T0265000-01; OS06G0265000.
GeneID4340706.
KEGGosa:4340706.

Organism-specific databases

GrameneQ43011.

Phylogenomic databases

eggNOGCOG0367.
HOGENOMHOG000027493.
KOK01953.
OMAWILAHER.

Enzyme and pathway databases

UniPathwayUPA00134; UER00195.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMSSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01536. asn_synth_AEB. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASNS2_ORYSJ
AccessionPrimary (citable) accession number: Q43011
Secondary accession number(s): Q0DD19, Q5Z6P2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways