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Q43011

- ASNS2_ORYSJ

UniProt

Q43011 - ASNS2_ORYSJ

Protein

Asparagine synthetase [glutamine-hydrolyzing] 2

Gene

Os06g0265000

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.By similarity

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei98 – 981GlutamineBy similarity
    Binding sitei231 – 2311ATP; via carbonyl oxygenBy similarity
    Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi341 – 3422ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
    2. aspartate-ammonia ligase activity Source: EnsemblPlants/Gramene
    3. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00134; UER00195.

    Protein family/group databases

    MEROPSiC44.976.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asparagine synthetase [glutamine-hydrolyzing] 2 (EC:6.3.5.4)
    Alternative name(s):
    Glutamine-dependent asparagine synthetase 2
    Gene namesi
    Ordered Locus Names:Os06g0265000, LOC_Os06g15420
    ORF Names:OJ1001_B06.12
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 6

    Organism-specific databases

    GrameneiQ43011.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: EnsemblPlants/Gramene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 591590Asparagine synthetase [glutamine-hydrolyzing] 2PRO_0000056925Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in companion cells of leaf sheath vascular bundles, and phloem-parenchyma cells, nucellar projections and nucellar epidermis of dorsal vascular bundles of grains.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ43011.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 185184Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini193 – 516324Asparagine synthetaseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni50 – 545Glutamine bindingBy similarity
    Regioni75 – 773Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 asparagine synthetase domain.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0367.
    HOGENOMiHOG000027493.
    KOiK01953.
    OMAiWILAHER.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q43011-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGILAVLGV ADVSLAKRSR IIELSRRLRH RGPDWSGIHC YQDCYLAHQR    50
    LAIVDPTSGD QPLYNEDKSV VVTVNGEIYN HEELKANLKS HKFQTASDCE 100
    VIAHLYEEYG EEFVDMLDGM FAFVLLDTRD KSFIAARDAI GICPLYMGWG 150
    LDGSVWFSSE MKALSDDCER FISFPPGHLY SSKTGGLRRW YNPPWFSESI 200
    PSTPYNPLLL RQSFEKAIIK RLMTDVPFGV LLSGGLDSSL VASVVSRHLA 250
    EAKVAAQWGN KLHTFCIGLK GSPDLRAAKE VADYLGTVHH ELHFTVQEGI 300
    DALEEVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKMVL SGEGSDEIFG 350
    GYLYFHKAPN KKEFHEETCR KIKALHLYDC LRANKSTSAW GVEARVPFLD 400
    KNFINVAMDI DPEWKMIKRD LGRIEKWVLR NAFDDEEKPY LPKHILYRQK 450
    EQFSDGVGYS WIDGLKDHAN EHVSDSMMMN ASFVYPENTP VTKEAYYYRT 500
    IFEKFFPKNA ARLTVPGGPS VACSTAKAVE WDAAWSKNLD PSGRAALGVH 550
    DAAYEDTLQK SPASANPVLD NGFGPALGES MVKTVASATA V 591
    Length:591
    Mass (Da):66,227
    Last modified:January 23, 2007 - v3
    Checksum:i3126C674724E4A3D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U55873 mRNA. Translation: AAB03991.1.
    D83378 mRNA. Translation: BAA18951.1.
    AP005382 Genomic DNA. Translation: BAD54377.1.
    AP008212 Genomic DNA. Translation: BAF19254.1.
    CM000143 Genomic DNA. Translation: EEE65484.1.
    AK100247 mRNA. Translation: BAG94512.1.
    PIRiT03602.
    RefSeqiNP_001057340.1. NM_001063875.1.
    UniGeneiOs.135.

    Genome annotation databases

    EnsemblPlantsiOS06T0265000-01; OS06T0265000-01; OS06G0265000.
    GeneIDi4340706.
    KEGGiosa:4340706.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U55873 mRNA. Translation: AAB03991.1 .
    D83378 mRNA. Translation: BAA18951.1 .
    AP005382 Genomic DNA. Translation: BAD54377.1 .
    AP008212 Genomic DNA. Translation: BAF19254.1 .
    CM000143 Genomic DNA. Translation: EEE65484.1 .
    AK100247 mRNA. Translation: BAG94512.1 .
    PIRi T03602.
    RefSeqi NP_001057340.1. NM_001063875.1.
    UniGenei Os.135.

    3D structure databases

    ProteinModelPortali Q43011.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C44.976.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS06T0265000-01 ; OS06T0265000-01 ; OS06G0265000 .
    GeneIDi 4340706.
    KEGGi osa:4340706.

    Organism-specific databases

    Gramenei Q43011.

    Phylogenomic databases

    eggNOGi COG0367.
    HOGENOMi HOG000027493.
    KOi K01953.
    OMAi WILAHER.

    Enzyme and pathway databases

    UniPathwayi UPA00134 ; UER00195 .

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Sueyoshi K., Kawachi T., Nakajima A., Yamagata H., Sugimoto T., Iwasaki T., Oji Y.
      Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Nipponbare.
      Tissue: Callus.
    2. "Nucleotide sequence of cDNA encoding asparagine synthetase from rice callus."
      Watanabe K., Higuchi T., Sakai T., Yamaya T.
      Plant Gene Register PGR96-020
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Nipponbare.
      Tissue: Callus.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    5. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    6. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.
    7. "Organ and cellular localization of asparagine synthetase in rice plants."
      Nakano K., Suzuki T., Hayakawa T., Yamaya T.
      Plant Cell Physiol. 41:874-880(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiASNS2_ORYSJ
    AccessioniPrimary (citable) accession number: Q43011
    Secondary accession number(s): Q0DD19, Q5Z6P2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 98 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3