Reviewed, UniProtKB/Swiss-Prot Q43011 (ASNS_ORYSJ)

Names and origin

Protein names

Recommended name:
    Asparagine synthetase [glutamine-hydrolyzing]
    EC=6.3.5.4
Alternative name(s):
    Glutamine-dependent asparagine synthetase

Gene names

Ordered Locus Names:	Os06g0265000, LOC_Os06g15420
ORF Names:	OJ1001_B06.12

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.
Sequence similarities	Contains 1 asparagine synthetase domain. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 591	590	Asparagine synthetase [glutamine-hydrolyzing]		PRO_0000056925
Regions
	Domain	2 – 185	184	Glutamine amidotransferase type-2
	Domain	193 – 516	324	Asparagine synthetase
Sites
	Active site	2	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q43011-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3126C674724E4A3D	FASTA	591	66,227
	10 20 30 40 50 60 MCGILAVLGV ADVSLAKRSR IIELSRRLRH RGPDWSGIHC YQDCYLAHQR LAIVDPTSGD 70 80 90 100 110 120 QPLYNEDKSV VVTVNGEIYN HEELKANLKS HKFQTASDCE VIAHLYEEYG EEFVDMLDGM 130 140 150 160 170 180 FAFVLLDTRD KSFIAARDAI GICPLYMGWG LDGSVWFSSE MKALSDDCER FISFPPGHLY 190 200 210 220 230 240 SSKTGGLRRW YNPPWFSESI PSTPYNPLLL RQSFEKAIIK RLMTDVPFGV LLSGGLDSSL 250 260 270 280 290 300 VASVVSRHLA EAKVAAQWGN KLHTFCIGLK GSPDLRAAKE VADYLGTVHH ELHFTVQEGI 310 320 330 340 350 360 DALEEVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKMVL SGEGSDEIFG GYLYFHKAPN 370 380 390 400 410 420 KKEFHEETCR KIKALHLYDC LRANKSTSAW GVEARVPFLD KNFINVAMDI DPEWKMIKRD 430 440 450 460 470 480 LGRIEKWVLR NAFDDEEKPY LPKHILYRQK EQFSDGVGYS WIDGLKDHAN EHVSDSMMMN 490 500 510 520 530 540 ASFVYPENTP VTKEAYYYRT IFEKFFPKNA ARLTVPGGPS VACSTAKAVE WDAAWSKNLD 550 560 570 580 590 PSGRAALGVH DAAYEDTLQK SPASANPVLD NGFGPALGES MVKTVASATA V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Sueyoshi K., Kawachi T., Nakajima A., Yamagata H., Sugimoto T., Iwasaki T., Oji Y. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare. Tissue: Callus.
[2]	"Nucleotide sequence of cDNA encoding asparagine synthetase from rice callus." Watanabe K., Higuchi T., Sakai T., Yamaya T. Plant Gene Register PGR96-020 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare. Tissue: Callus.
[3]	"Oryza sativa nipponbare chromosome 6 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U55873 mRNA. Translation: AAB03991.1. D83378 mRNA. Translation: BAA18951.1. AP005382 Genomic DNA. Translation: BAD54377.1.
PIR	T03602.
RefSeq	NP_001057340.1.
UniGene	Os.135
3D structure databases
HSSP	HSSP built from PDB template 1CT9 based on UniProtKB P22106.
ModBase	Search...
Protein family/group databases
MEROPS	C44.974.
Genome annotation databases
GeneID	4340706.
KEGG	osa:4340706.
Organism-specific databases
Gramene	Q43011.
Family and domain databases
InterPro	IPR006426. Asn_synth_AEB. IPR001962. Asn_synthase. IPR000583. GATase_2. IPR017932. GATase_II. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00733. Asn_synthase. 1 hit. PF00310. GATase_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01536. asn_synth_AEB. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNS_ORYSJ

Accession

Primary (citable) accession number: Q43011
Secondary accession number(s): Q5Z6P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	September 22, 2009
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)