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Q43011

- ASNS2_ORYSJ

UniProt

Q43011 - ASNS2_ORYSJ

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Protein

Asparagine synthetase [glutamine-hydrolyzing] 2

Gene

Os06g0265000

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.By similarity

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei98 – 981GlutamineBy similarity
Binding sitei231 – 2311ATP; via carbonyl oxygenBy similarity
Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygenBy similarity
Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi341 – 3422ATPBy similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 2 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 2
Gene namesi
Ordered Locus Names:Os06g0265000, LOC_Os06g15420
ORF Names:OJ1001_B06.12
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 6

Organism-specific databases

GrameneiQ43011.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 591590Asparagine synthetase [glutamine-hydrolyzing] 2PRO_0000056925Add
BLAST

Expressioni

Tissue specificityi

Expressed in companion cells of leaf sheath vascular bundles, and phloem-parenchyma cells, nucellar projections and nucellar epidermis of dorsal vascular bundles of grains.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ43011.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 185184Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini193 – 516324Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine bindingBy similarity
Regioni75 – 773Glutamine bindingBy similarity

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0367.
HOGENOMiHOG000027493.
InParanoidiQ43011.
KOiK01953.
OMAiWILAHER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43011-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGILAVLGV ADVSLAKRSR IIELSRRLRH RGPDWSGIHC YQDCYLAHQR
60 70 80 90 100
LAIVDPTSGD QPLYNEDKSV VVTVNGEIYN HEELKANLKS HKFQTASDCE
110 120 130 140 150
VIAHLYEEYG EEFVDMLDGM FAFVLLDTRD KSFIAARDAI GICPLYMGWG
160 170 180 190 200
LDGSVWFSSE MKALSDDCER FISFPPGHLY SSKTGGLRRW YNPPWFSESI
210 220 230 240 250
PSTPYNPLLL RQSFEKAIIK RLMTDVPFGV LLSGGLDSSL VASVVSRHLA
260 270 280 290 300
EAKVAAQWGN KLHTFCIGLK GSPDLRAAKE VADYLGTVHH ELHFTVQEGI
310 320 330 340 350
DALEEVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKMVL SGEGSDEIFG
360 370 380 390 400
GYLYFHKAPN KKEFHEETCR KIKALHLYDC LRANKSTSAW GVEARVPFLD
410 420 430 440 450
KNFINVAMDI DPEWKMIKRD LGRIEKWVLR NAFDDEEKPY LPKHILYRQK
460 470 480 490 500
EQFSDGVGYS WIDGLKDHAN EHVSDSMMMN ASFVYPENTP VTKEAYYYRT
510 520 530 540 550
IFEKFFPKNA ARLTVPGGPS VACSTAKAVE WDAAWSKNLD PSGRAALGVH
560 570 580 590
DAAYEDTLQK SPASANPVLD NGFGPALGES MVKTVASATA V
Length:591
Mass (Da):66,227
Last modified:January 23, 2007 - v3
Checksum:i3126C674724E4A3D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U55873 mRNA. Translation: AAB03991.1.
D83378 mRNA. Translation: BAA18951.1.
AP005382 Genomic DNA. Translation: BAD54377.1.
AP008212 Genomic DNA. Translation: BAF19254.1.
CM000143 Genomic DNA. Translation: EEE65484.1.
AK100247 mRNA. Translation: BAG94512.1.
PIRiT03602.
RefSeqiNP_001057340.1. NM_001063875.1.
UniGeneiOs.135.

Genome annotation databases

EnsemblPlantsiOS06T0265000-01; OS06T0265000-01; OS06G0265000.
GeneIDi4340706.
KEGGiosa:4340706.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U55873 mRNA. Translation: AAB03991.1 .
D83378 mRNA. Translation: BAA18951.1 .
AP005382 Genomic DNA. Translation: BAD54377.1 .
AP008212 Genomic DNA. Translation: BAF19254.1 .
CM000143 Genomic DNA. Translation: EEE65484.1 .
AK100247 mRNA. Translation: BAG94512.1 .
PIRi T03602.
RefSeqi NP_001057340.1. NM_001063875.1.
UniGenei Os.135.

3D structure databases

ProteinModelPortali Q43011.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.976.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS06T0265000-01 ; OS06T0265000-01 ; OS06G0265000 .
GeneIDi 4340706.
KEGGi osa:4340706.

Organism-specific databases

Gramenei Q43011.

Phylogenomic databases

eggNOGi COG0367.
HOGENOMi HOG000027493.
InParanoidi Q43011.
KOi K01953.
OMAi WILAHER.

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Sueyoshi K., Kawachi T., Nakajima A., Yamagata H., Sugimoto T., Iwasaki T., Oji Y.
    Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Nipponbare.
    Tissue: Callus.
  2. "Nucleotide sequence of cDNA encoding asparagine synthetase from rice callus."
    Watanabe K., Higuchi T., Sakai T., Yamaya T.
    Plant Gene Register PGR96-020
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Nipponbare.
    Tissue: Callus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  6. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  7. "Organ and cellular localization of asparagine synthetase in rice plants."
    Nakano K., Suzuki T., Hayakawa T., Yamaya T.
    Plant Cell Physiol. 41:874-880(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiASNS2_ORYSJ
AccessioniPrimary (citable) accession number: Q43011
Secondary accession number(s): Q0DD19, Q5Z6P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3