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Protein

Asparagine synthetase [glutamine-hydrolyzing] 2

Gene

Os06g0265000

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.By similarity

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Asparagine synthetase [glutamine-hydrolyzing] 2 (Os06g0265000)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei98GlutamineBy similarity1
Binding sitei231ATP; via carbonyl oxygenBy similarity1
Binding sitei267ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei343Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi341 – 342ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Asparagine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-OSA-70614 Amino acid synthesis and interconversion (transamination)
UniPathwayiUPA00134; UER00195

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 2 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 2
Gene namesi
Ordered Locus Names:Os06g0265000, LOC_Os06g15420
ORF Names:OJ1001_B06.12
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 6

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000569252 – 591Asparagine synthetase [glutamine-hydrolyzing] 2Add BLAST590

Proteomic databases

PaxDbiQ43011
PRIDEiQ43011

Expressioni

Tissue specificityi

Expressed in companion cells of leaf sheath vascular bundles, and phloem-parenchyma cells, nucellar projections and nucellar epidermis of dorsal vascular bundles of grains.1 Publication

Gene expression databases

ExpressionAtlasiQ43011 differential
GenevisibleiQ43011 OS

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi39947.LOC_Os06g15420.1

Structurei

3D structure databases

ProteinModelPortaliQ43011
SMRiQ43011
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 185Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST184
Domaini193 – 516Asparagine synthetaseAdd BLAST324

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 54Glutamine bindingBy similarity5
Regioni75 – 77Glutamine bindingBy similarity3

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG0571 Eukaryota
COG0367 LUCA
HOGENOMiHOG000027493
InParanoidiQ43011
KOiK01953
OMAiYFHAAPD
OrthoDBiEOG093604QK

Family and domain databases

CDDicd01991 Asn_Synthase_B_C, 1 hit
cd00712 AsnB, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR006426 Asn_synth_AEB
IPR001962 Asn_synthase
IPR033738 AsnB_N
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00733 Asn_synthase, 1 hit
PF13537 GATase_7, 1 hit
PIRSFiPIRSF001589 Asn_synthetase_glu-h, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01536 asn_synth_AEB, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGILAVLGV ADVSLAKRSR IIELSRRLRH RGPDWSGIHC YQDCYLAHQR
60 70 80 90 100
LAIVDPTSGD QPLYNEDKSV VVTVNGEIYN HEELKANLKS HKFQTASDCE
110 120 130 140 150
VIAHLYEEYG EEFVDMLDGM FAFVLLDTRD KSFIAARDAI GICPLYMGWG
160 170 180 190 200
LDGSVWFSSE MKALSDDCER FISFPPGHLY SSKTGGLRRW YNPPWFSESI
210 220 230 240 250
PSTPYNPLLL RQSFEKAIIK RLMTDVPFGV LLSGGLDSSL VASVVSRHLA
260 270 280 290 300
EAKVAAQWGN KLHTFCIGLK GSPDLRAAKE VADYLGTVHH ELHFTVQEGI
310 320 330 340 350
DALEEVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKMVL SGEGSDEIFG
360 370 380 390 400
GYLYFHKAPN KKEFHEETCR KIKALHLYDC LRANKSTSAW GVEARVPFLD
410 420 430 440 450
KNFINVAMDI DPEWKMIKRD LGRIEKWVLR NAFDDEEKPY LPKHILYRQK
460 470 480 490 500
EQFSDGVGYS WIDGLKDHAN EHVSDSMMMN ASFVYPENTP VTKEAYYYRT
510 520 530 540 550
IFEKFFPKNA ARLTVPGGPS VACSTAKAVE WDAAWSKNLD PSGRAALGVH
560 570 580 590
DAAYEDTLQK SPASANPVLD NGFGPALGES MVKTVASATA V
Length:591
Mass (Da):66,227
Last modified:January 23, 2007 - v3
Checksum:i3126C674724E4A3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55873 mRNA Translation: AAB03991.1
D83378 mRNA Translation: BAA18951.1
AP005382 Genomic DNA Translation: BAD54377.1
AP008212 Genomic DNA Translation: BAF19254.1
AP014962 Genomic DNA Translation: BAS97145.1
CM000143 Genomic DNA Translation: EEE65484.1
AK100247 mRNA Translation: BAG94512.1
PIRiT03602
RefSeqiXP_015643183.1, XM_015787697.1
UniGeneiOs.135

Genome annotation databases

EnsemblPlantsiOs06t0265000-01; Os06t0265000-01; Os06g0265000
GeneIDi4340706
GrameneiOs06t0265000-01; Os06t0265000-01; Os06g0265000
KEGGiosa:4340706

Similar proteinsi

Entry informationi

Entry nameiASNS2_ORYSJ
AccessioniPrimary (citable) accession number: Q43011
Secondary accession number(s): Q0DD19, Q5Z6P2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 120 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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