Reviewed, UniProtKB/Swiss-Prot Q43007 (PLDA1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Phospholipase D alpha 1
      Short name=PLD alpha 1
    EC=3.1.4.4
Alternative name(s):
    Choline phosphatase 1
    Phosphatidylcholine-hydrolyzing phospholipase D 1

Gene names

Name:	PLD1
Ordered Locus Names:	Os01g0172400, LOC_Os01g07760

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	812 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Cofactor	Calcium.
Subunit structure	Monomer.
Tissue specificity	Expressed in leaves, roots, developing seeds and cultured cells.
Developmental stage	The transcript begins to emerged in seeds as early as the second day after imbibition and increased after radicle emergence on the third day. Strongly expressed in the leaves, roots and residual grain of seedling eight days after imbibition. Undetectable in mature dry seeds.
Domain	C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.
Sequence similarities	Belongs to the phospholipase D family. C2-PLD subfamily. Contains 1 C2 domain. Contains 2 PLD phosphodiesterase domains.
biophysicochemical properties	pH dependence: Optimum pH is 6.0. Temperature dependence: Stable from 4 to 37 degrees Celsius. Activity is reduced to one third of the original level after incubation at 50 degrees Celsius for 30 minutes.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Repeat
Ligand	Calcium
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylcholine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 46	46			PRO_0000024655
	Chain	47 – 812	766	Phospholipase D alpha 1		PRO_0000024656
Regions
	Domain	1 – 114	114	C2
	Domain	330 – 368	39	PLD phosphodiesterase 1
	Domain	658 – 685	28	PLD phosphodiesterase 2
Sites
	Active site	335	1	Potential
	Active site	337	1	Potential
	Active site	342	1	Potential
	Active site	663	1	Potential
	Active site	665	1	Potential
	Active site	670	1	Potential
Experimental info
	Sequence conflict	139	1	P → S Ref.1
	Sequence conflict	139	1	P → S Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q43007-1 [UniParc]. Last modified June 13, 2006. Version 2. Checksum: 6AE267E6C8B1DC5E	FASTA	812	92,219
	10 20 30 40 50 60 MAQMLLHGTL HATIFEAASL SNPHRASGSA PKFIRKFVEG IEDTVGVGKG ATKVYSTIDL 70 80 90 100 110 120 EKARVGRTRM ITNEPINPRW YESFHIYCAH MASNVIFTVK IDNPIGATNI GRAYLPVQEL 130 140 150 160 170 180 LNGEEIDRWL DICDNNREPV GESKIHVKLQ YFDVSKDRNW ARGVRSTKYP GVPYTFFSQR 190 200 210 220 230 240 QGCKVTLYQD AHVPDNFIPK IPLADGKNYE PHRCWEDIFD AISNAQHLIY ITGWSVYTEI 250 260 270 280 290 300 TLVRDSNRPK PGGDVTLGEL LKKKASEGVR VLMLVWDDRT SVGLLKRDGL MATHDEETEN 310 320 330 340 350 360 YFHGSDVNCV LCPRNPDDSG SIVQDLSIST MFTHHQKIVV VDHELPNQGS QQRRIVSFVG 370 380 390 400 410 420 GLDLCDGRYD TQYHSLFRTL DSTHHDDFHQ PNFATASIKK GGPREPWHDI HSRLEGPIAW 430 440 450 460 470 480 DVLYNFEQRW RKQGGKDLLL QLRDLSDTII PPSPVMFPED RETWNVQLFR SIDGGAAFGF 490 500 510 520 530 540 PDTPEEAAKA GLVSGKDQII DRSIQDAYIH AIRRAKNFIY IENQYFLGSS YAWKPEGIKP 550 560 570 580 590 600 EDIGALHLIP KELALKVVSK IEAGERFTVY VVVPMWPEGV PESGSVQAIL DWQRRTMEMM 610 620 630 640 650 660 YTDITEALQA KGIEANPKDY LTFFCLGNRE VKQAGEYQPE EQPEADTDYS RAQEARRFMI 670 680 690 700 710 720 YVHTKMMIVD DEYIIIGSAN INQRSMDGAR DSEIAMGGYQ PYHLATRQPA RGQIHGFRMA 730 740 750 760 770 780 LWYEHLGMLD DVFQRPESLE CVQKVNRIAE KYWDMYSSDD LQQDLPGHLL SYPIGVASDG 790 800 810 VVTELPGMEY FPDTRARVLG AKSDYMPPIL TS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification and characterization of phospholipase D (PLD) from rice (Oryza sativa L.) and cloning of cDNA for PLD from rice and maize (Zea mays L.)." Ueki J., Morioka S., Komari T., Kumashiro T. Plant Cell Physiol. 36:903-914(1995) [PubMed: 7551587] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Koshihikari.
[2]	"Characterization of two Distinctive genomic clones for phospholipase D from rice." Morioka S., Ueki J., Komari T. Plant Gene Register PGR97-076 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Koshihikari. Tissue: Leaf.
[3]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D73411 mRNA. Translation: BAA11136.1. AB001920 Genomic DNA. Translation: BAA19467.1. AP003215 Genomic DNA. No translation available. AP003282 Genomic DNA. No translation available.
PIR	T03402.
RefSeq	NP_001042153.1.
UniGene	Os.155
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4327647.
KEGG	osa:4327647.
Organism-specific databases
Gramene	Q43007.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR015679. Phospholipase_D. IPR011402. PLD_pln. IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00168. C2. 1 hit. PF00614. PLDc. 2 hits. [Graphical view]
PIRSF	PIRSF036470. PLD_plant. 1 hit.
SMART	SM00239. C2. 1 hit. SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50004. C2. False negative. PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLDA1_ORYSJ

Accession

Primary (citable) accession number: Q43007

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 13, 2006
Last modified:	June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)