Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phospholipase D alpha 1

Gene

PLD1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes.

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.

Cofactori

pH dependencei

Optimum pH is 6.0.

Temperature dependencei

Stable from 4 to 37 degrees Celsius. Activity is reduced to one third of the original level after incubation at 50 degrees Celsius for 30 minutes.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei335PROSITE-ProRule annotation1
Active sitei337PROSITE-ProRule annotation1
Active sitei342PROSITE-ProRule annotation1
Active sitei663PROSITE-ProRule annotation1
Active sitei665PROSITE-ProRule annotation1
Active sitei670PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism
LigandCalcium

Enzyme and pathway databases

ReactomeiR-OSA-1483166 Synthesis of PA
R-OSA-2029485 Role of phospholipids in phagocytosis
R-OSA-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D alpha 1 (EC:3.1.4.4)
Short name:
PLD alpha 1
Alternative name(s):
Choline phosphatase 1
Phosphatidylcholine-hydrolyzing phospholipase D 1
Gene namesi
Name:PLD1
Ordered Locus Names:Os01g0172400Imported, LOC_Os01g07760Curated
ORF Names:OsJ_00559Imported
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 1

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000246551 – 46Add BLAST46
ChainiPRO_000002465647 – 812Phospholipase D alpha 1Add BLAST766

Proteomic databases

PaxDbiQ43007
PRIDEiQ43007

Expressioni

Tissue specificityi

Expressed in leaves, roots, developing seeds and cultured cells.

Developmental stagei

The transcript begins to emerged in seeds as early as the second day after imbibition and increased after radicle emergence on the third day. Strongly expressed in the leaves, roots and residual grain of seedling eight days after imbibition. Undetectable in mature dry seeds.

Gene expression databases

ExpressionAtlasiQ43007 differential
GenevisibleiQ43007 OS

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi39947.LOC_Os01g07760.1

Structurei

3D structure databases

ProteinModelPortaliQ43007
SMRiQ43007
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 114C2Add BLAST114
Domaini330 – 368PLD phosphodiesterase 1PROSITE-ProRule annotationAdd BLAST39
Domaini658 – 685PLD phosphodiesterase 2PROSITE-ProRule annotationAdd BLAST28

Domaini

C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1329 Eukaryota
COG1502 LUCA
HOGENOMiHOG000240112
InParanoidiQ43007
KOiK01115
OMAiGTKSDYM
OrthoDBiEOG093601XZ

Family and domain databases

Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR001736 PLipase_D/transphosphatidylase
IPR024632 PLipase_D_C
IPR015679 PLipase_D_fam
IPR011402 PLipase_D_pln
PANTHERiPTHR18896 PTHR18896, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF12357 PLD_C, 1 hit
PF00614 PLDc, 2 hits
PIRSFiPIRSF036470 PLD_plant, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00155 PLDc, 2 hits
PROSITEiView protein in PROSITE
PS50035 PLD, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q43007-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQMLLHGTL HATIFEAASL SNPHRASGSA PKFIRKFVEG IEDTVGVGKG
60 70 80 90 100
ATKVYSTIDL EKARVGRTRM ITNEPINPRW YESFHIYCAH MASNVIFTVK
110 120 130 140 150
IDNPIGATNI GRAYLPVQEL LNGEEIDRWL DICDNNREPV GESKIHVKLQ
160 170 180 190 200
YFDVSKDRNW ARGVRSTKYP GVPYTFFSQR QGCKVTLYQD AHVPDNFIPK
210 220 230 240 250
IPLADGKNYE PHRCWEDIFD AISNAQHLIY ITGWSVYTEI TLVRDSNRPK
260 270 280 290 300
PGGDVTLGEL LKKKASEGVR VLMLVWDDRT SVGLLKRDGL MATHDEETEN
310 320 330 340 350
YFHGSDVNCV LCPRNPDDSG SIVQDLSIST MFTHHQKIVV VDHELPNQGS
360 370 380 390 400
QQRRIVSFVG GLDLCDGRYD TQYHSLFRTL DSTHHDDFHQ PNFATASIKK
410 420 430 440 450
GGPREPWHDI HSRLEGPIAW DVLYNFEQRW RKQGGKDLLL QLRDLSDTII
460 470 480 490 500
PPSPVMFPED RETWNVQLFR SIDGGAAFGF PDTPEEAAKA GLVSGKDQII
510 520 530 540 550
DRSIQDAYIH AIRRAKNFIY IENQYFLGSS YAWKPEGIKP EDIGALHLIP
560 570 580 590 600
KELALKVVSK IEAGERFTVY VVVPMWPEGV PESGSVQAIL DWQRRTMEMM
610 620 630 640 650
YTDITEALQA KGIEANPKDY LTFFCLGNRE VKQAGEYQPE EQPEADTDYS
660 670 680 690 700
RAQEARRFMI YVHTKMMIVD DEYIIIGSAN INQRSMDGAR DSEIAMGGYQ
710 720 730 740 750
PYHLATRQPA RGQIHGFRMA LWYEHLGMLD DVFQRPESLE CVQKVNRIAE
760 770 780 790 800
KYWDMYSSDD LQQDLPGHLL SYPIGVASDG VVTELPGMEY FPDTRARVLG
810
AKSDYMPPIL TS
Length:812
Mass (Da):92,219
Last modified:June 13, 2006 - v2
Checksum:i6AE267E6C8B1DC5E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139P → S (PubMed:7551587).Curated1
Sequence conflicti139P → S (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D73411 mRNA Translation: BAA11136.1
AB001920 Genomic DNA Translation: BAA19467.1
AP003215 Genomic DNA No translation available.
AP003282 Genomic DNA No translation available.
AP008207 Genomic DNA Translation: BAF04067.1
AP014957 Genomic DNA Translation: BAS70634.1
CM000138 Genomic DNA Translation: EEE53959.1
PIRiT03402
RefSeqiXP_015646609.1, XM_015791123.1
UniGeneiOs.155

Genome annotation databases

EnsemblPlantsiOs01t0172400-02; Os01t0172400-02; Os01g0172400
Os01t0172400-03; Os01t0172400-03; Os01g0172400
GeneIDi4327647
GrameneiOs01t0172400-02; Os01t0172400-02; Os01g0172400
Os01t0172400-03; Os01t0172400-03; Os01g0172400
KEGGiosa:4327647

Similar proteinsi

Entry informationi

Entry nameiPLDA1_ORYSJ
AccessioniPrimary (citable) accession number: Q43007
Secondary accession number(s): A0A0P0UZ48, Q0JQB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 13, 2006
Last modified: May 23, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health