ID   NIR_ORYSJ               Reviewed;         596 AA.
AC   Q42997; Q5ZBK4; Q7F475;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 52.
DE   RecName: Full=Ferredoxin--nitrite reductase, chloroplastic;
DE            EC=1.7.7.1;
DE   Flags: Precursor;
GN   OrderedLocusNames=Os01g0357100, LOC_Os01g25484;
GN   ORFNames=P0025H06.19-1, P0025H06.19-2;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade;
OC   Ehrhartoideae; Oryzeae; Oryza.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Kinmaze; TISSUE=Leaf;
RX   MEDLINE=96100954; PubMed=8541663;
RA   Terada Y., Aoki H., Tanaka T., Morikawa H., Ida S.;
RT   "Cloning and nucleotide sequence of a leaf ferredoxin-nitrite
RT   reductase cDNA of rice.";
RL   Biosci. Biotechnol. Biochem. 59:2183-2185(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   MEDLINE=22337376; PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M.,
RA   Okamoto M., Ando T., Aoki H., Arita K., Hamada M., Harada C.,
RA   Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M.,
RA   Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K.,
RA   Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I.,
RA   Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y.,
RA   Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M.,
RA   Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M.,
RA   Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K.,
RA   Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K.,
RA   Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y.,
RA   Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
CC   -!- FUNCTION: Catalyzes the six-electron reduction of nitrite to
CC       ammonium.
CC   -!- CATALYTIC ACTIVITY: NH(3) + 2 H(2)O + 6 oxidized ferredoxin =
CC       nitrite + 6 reduced ferredoxin + 7 H(+).
CC   -!- COFACTOR: Binds 1 siroheme per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
CC   -!- PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast (Probable).
CC   -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S
CC       domain family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAD53072.1; Type=Erroneous gene model prediction;
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DR   EMBL; D50556; BAA09122.1; -; mRNA.
DR   EMBL; AP003312; BAC10721.1; -; Genomic_DNA.
DR   EMBL; AP003312; BAD53072.1; ALT_SEQ; Genomic_DNA.
DR   PIR; JC4395; JC4395.
DR   SMR; Q42997; 55-589.
DR   NMPDR; fig|39947.1.peg.10934; -.
DR   Gramene; Q42997; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0048307; F:ferredoxin-nitrite reductase activity; IEA:EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   GO; GO:0006810; P:transport; IEA:UniProtKB-KW.
DR   InterPro; IPR006066; Nir_Si_BS.
DR   InterPro; IPR006067; Nir_Sir_4Fe4S.
DR   InterPro; IPR005117; NiRdtase/SiRdtase_haem-b_fer.
DR   Pfam; PF01077; NIR_SIR; 1.
DR   Pfam; PF03460; NIR_SIR_ferr; 2.
DR   PRINTS; PR00397; SIROHAEM.
DR   PROSITE; PS00365; NIR_SIR; 1.
PE   2: Evidence at transcript level;
KW   4Fe-4S; Chloroplast; Electron transport; Heme; Iron; Iron-sulfur;
KW   Metal-binding; Nitrate assimilation; Oxidoreductase; Plastid; Repeat;
KW   Transit peptide; Transport.
FT   TRANSIT       1     28       Chloroplast (Potential).
FT   CHAIN        29    596       Ferredoxin--nitrite reductase,
FT                                chloroplastic.
FT                                /FTId=PRO_0000247480.
FT   METAL       474    474       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       480    480       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       515    515       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       519    519       Iron (siroheme axial ligand) (By
FT                                similarity).
FT   METAL       519    519       Iron-sulfur (4Fe-4S) (By similarity).
SQ   SEQUENCE   596 AA;  66143 MW;  B69BC7FCD11390F5 CRC64;
     MASSASLQRF LPPYPHAAAS RCRPPGVRAR PVQSSTVSAP SSSTPAADEA VSAERLEPRV
     EQREGRYWVL KEKYRTGLNP QEKVKLGKEP MSLFMEGGIK ELAKMPMEEI EADKLSKEDI
     DVRLKWLGLF HRRKHQYGRF MMRLKLPNGV TTSEQTRYLA SVIEAYGKEG CADVTTRQNW
     QIRGVTLPDV PAILDGLNAV GLTSLQSGMD NVRNPVGNPL AGIDPDEIVD TRSYTNLLSS
     YITSNFQGNP TITNLPRKWN VCVIGSHDLY EHPHINDLAY MPAVKGGKFG FNLLVGGFIS
     PKRWEEALPL DAWVPGDDII PVCKAVLEAY RDLGTRGNRQ KTRMMWLIDE LGMEAFRSEV
     EKRMPNGVLE RAAPEDLIDK KWQRRDYLGV HPQKQEGMSY VGLHVPVGRV QAADMFELAR
     LADEYGSGEL RLTVEQNIVI PNVKNEKVEA LLSEPLLQKF SPQPSLLLKG LVACTGNQFC
     GQAIIETKQR ALLVTSQVEK LVSVPRAVRM HWTGCPNSCG QVQVADIGFM GCLTKDSAGK
     IVEAADIFVG GRVGSDSHLA GAYKKSVPCD ELAPIVADIL VERFGAVRRE REEDEE
//