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Protein

Ferredoxin--nitrite reductase, chloroplastic

Gene

Os01g0357100

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the six-electron reduction of nitrite to ammonium.

Catalytic activityi

NH3 + 2 H2O + 6 oxidized ferredoxin = nitrite + 6 reduced ferredoxin + 7 H+.

Cofactori

Protein has several cofactor binding sites:
  • sirohemeBy similarityNote: Binds 1 siroheme per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathwayi: nitrate reduction (assimilation)

This protein is involved in the pathway nitrate reduction (assimilation), which is part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrate reduction (assimilation) and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi474 – 4741Iron-sulfur (4Fe-4S)By similarity
Metal bindingi480 – 4801Iron-sulfur (4Fe-4S)By similarity
Metal bindingi515 – 5151Iron-sulfur (4Fe-4S)By similarity
Metal bindingi519 – 5191Iron (siroheme axial ligand)By similarity
Metal bindingi519 – 5191Iron-sulfur (4Fe-4S)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00653.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferredoxin--nitrite reductase, chloroplastic (EC:1.7.7.1)
Gene namesi
Ordered Locus Names:Os01g0357100, LOC_Os01g25484
ORF Names:P0025H06.19-1, P0025H06.19-2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2828ChloroplastSequence analysisAdd
BLAST
Chaini29 – 596568Ferredoxin--nitrite reductase, chloroplasticPRO_0000247480Add
BLAST

Proteomic databases

PaxDbiQ42997.

Expressioni

Gene expression databases

GenevisibleiQ42997. OS.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os02g52730.1.

Structurei

3D structure databases

ProteinModelPortaliQ42997.
SMRiQ42997. Positions 55-589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0560. Eukaryota.
COG0155. LUCA.
HOGENOMiHOG000249408.
InParanoidiQ42997.

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
InterProiIPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
PROSITEiPS00365. NIR_SIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42997-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSASLQRF LPPYPHAAAS RCRPPGVRAR PVQSSTVSAP SSSTPAADEA
60 70 80 90 100
VSAERLEPRV EQREGRYWVL KEKYRTGLNP QEKVKLGKEP MSLFMEGGIK
110 120 130 140 150
ELAKMPMEEI EADKLSKEDI DVRLKWLGLF HRRKHQYGRF MMRLKLPNGV
160 170 180 190 200
TTSEQTRYLA SVIEAYGKEG CADVTTRQNW QIRGVTLPDV PAILDGLNAV
210 220 230 240 250
GLTSLQSGMD NVRNPVGNPL AGIDPDEIVD TRSYTNLLSS YITSNFQGNP
260 270 280 290 300
TITNLPRKWN VCVIGSHDLY EHPHINDLAY MPAVKGGKFG FNLLVGGFIS
310 320 330 340 350
PKRWEEALPL DAWVPGDDII PVCKAVLEAY RDLGTRGNRQ KTRMMWLIDE
360 370 380 390 400
LGMEAFRSEV EKRMPNGVLE RAAPEDLIDK KWQRRDYLGV HPQKQEGMSY
410 420 430 440 450
VGLHVPVGRV QAADMFELAR LADEYGSGEL RLTVEQNIVI PNVKNEKVEA
460 470 480 490 500
LLSEPLLQKF SPQPSLLLKG LVACTGNQFC GQAIIETKQR ALLVTSQVEK
510 520 530 540 550
LVSVPRAVRM HWTGCPNSCG QVQVADIGFM GCLTKDSAGK IVEAADIFVG
560 570 580 590
GRVGSDSHLA GAYKKSVPCD ELAPIVADIL VERFGAVRRE REEDEE
Length:596
Mass (Da):66,143
Last modified:November 1, 1996 - v1
Checksum:iB69BC7FCD11390F5
GO

Sequence cautioni

The sequence BAD53072.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50556 mRNA. Translation: BAA09122.1.
AP003312 Genomic DNA. Translation: BAC10721.1.
AP003312 Genomic DNA. Translation: BAD53072.1. Sequence problems.
AP014957 Genomic DNA. No translation available.
PIRiJC4395.
RefSeqiXP_015641702.1. XM_015786216.1.
UniGeneiOs.142.

Genome annotation databases

GeneIDi4326014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50556 mRNA. Translation: BAA09122.1.
AP003312 Genomic DNA. Translation: BAC10721.1.
AP003312 Genomic DNA. Translation: BAD53072.1. Sequence problems.
AP014957 Genomic DNA. No translation available.
PIRiJC4395.
RefSeqiXP_015641702.1. XM_015786216.1.
UniGeneiOs.142.

3D structure databases

ProteinModelPortaliQ42997.
SMRiQ42997. Positions 55-589.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os02g52730.1.

Proteomic databases

PaxDbiQ42997.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4326014.

Phylogenomic databases

eggNOGiKOG0560. Eukaryota.
COG0155. LUCA.
HOGENOMiHOG000249408.
InParanoidiQ42997.

Enzyme and pathway databases

UniPathwayiUPA00653.

Gene expression databases

GenevisibleiQ42997. OS.

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
InterProiIPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
PROSITEiPS00365. NIR_SIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and nucleotide sequence of a leaf ferredoxin-nitrite reductase cDNA of rice."
    Terada Y., Aoki H., Tanaka T., Morikawa H., Ida S.
    Biosci. Biotechnol. Biochem. 59:2183-2185(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Kinmaze.
    Tissue: Leaf.
  2. "The genome sequence and structure of rice chromosome 1."
    Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
    , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
    Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiNIR_ORYSJ
AccessioniPrimary (citable) accession number: Q42997
Secondary accession number(s): Q5ZBK4, Q7F475
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.